ISSN: 2536-7080
Model: Open Access/Peer Reviewed
DOI: 10.31248/RJFSN
Start Year: 2016
Email: rjfsn@integrityresjournals.org
https://doi.org/10.31248/RJFSN2019.064 | Article Number: 83AA19D11 | Vol.4 (6) - December 2019
Received Date: 30 January 2019 | Accepted Date: 05 December 2019 | Published Date: 30 December 2019
Authors: Afieroho O. E.* , AJuzie J. I. and Afieroho M. C.
Keywords: proximate composition, Harungana madagascariensis, under-utilized fruits, functional foods.
This study reports the proximate composition and some antioxidant properties: free radical scavenging (FRS), total phenolics content (TPC) and total flavonoids content (TFC) of the under-utilized fresh fruits of Harungana madagascariensis, a plant widely used in folklore medicine. The proximate compositions were determined following the AOAC method. The antioxidant properties FRS, TPC and TFC were respectively evaluated in vitro using the standard diphenyl picryl hydrazine (DPPH), Folin ciocateau and Aluminium chloride spectrophotometric assays. The proximate compositions: water content (13.771 g/100g fresh fruit), dry matter (82.229 g/100g fresh fruit), ash (3.087 g/100g fresh fruit), crude fat (42.011 g/100g fresh fruit), crude protein (6.489 g/100g fresh fruit), crude fibre (0.396 g/100g fresh fruit), total carbohydrate (34.246 g/100g fresh fruit) and Energy value (541.036 Kcal/100 g fresh fruit) were obtained from the fruit of H. madagascariensis. For the antioxidant properties, a TPC (6.758 mg tannic acid equivalent per g fresh fruit), TFC (0.055 mg quercetin equivalent/g fresh fruit) and FRS (IC50 = 0.05 mg/mL) were obtained. This study is reporting for the first time the proximate composition of the fruits of H. madagascariensis a plant widely used in ethno-medicine in addition to its antioxidant potentials and recommends it as a good source of functional food.
Afieroho, O. E. & Afieroho, M. C. (2017). Evaluation of the nitric oxide scavenging potential of Harunguna madagascariensis Lam ex poir (Hypericaceae) fruits oil. The Pharma Innovation Journal, 6(2), 171-173. | ||||
Afieroho, O. E, Izontimi, S. S., Okoroafor, D. O., & Caleb, B. (2012). Antibacterial and phytochemical evaluation of Harungana madagascariensis (Hypericaceae) seeds. International Research Journal of Pharmacy, 3(11), 75-77. | ||||
Afieroho, O. E., Okonkwo, T. J. N., Shorinwa, O. A., Okonkwo, C. J., Ajibade, S., & Afieroho, M. C. (2017). Harungana madagascariensis Lam ex poir (Hypericaceae) fruits oil extract: Phytochemistry and acute toxicity evaluation. International Journal for Pharmaceutical Sciences and Research, 8(6), 2539-2544. | ||||
Agnaniet, H., Mbot, E. J., Keita, O., Fehrentz, J., Ankli, A., Gallud, A., Garcia, M., Gary-Bobo, M., Lebibi, J., Cresteil, T., & Menut, C. (2016). Antidiabetic potential of two medicinal plants used in Gabonese folk medicine. BMC Complementary and Alternative Medicine, 16, 71. Crossref |
||||
Ajah, P. O., &.Madubuike, F. N. (1997). The proximate composition of some tropical legume seeds grown in two states in Nigeria. Food Chemistry, 59(3), 361-365. Crossref |
||||
Antia, B. S., Ita, B. N., & Udo, U. E. (2015). Nutrient composition and in vitro antioxidant properties of Harungana madagascariensis stem bark extracts. Journal of Medicinal Food, 18(5), 609-614. Crossref |
||||
AOAC (2000). Official Methods of Analysis. 17th Edition. The Association of Official Analytical Chemists, Gaithersburg, MD, USA. Methods 925.10, 65.17, 974.24, 992.16. | ||||
Biapa, P. N., Matei, H., Bâlici, S., Oben, J. E., & Ngogang, J. Y. (2013). Protective effects of stem bark of Harungana madgascariensis on the red blood cell membrane. BMC Complementary and Alternative Medicine, 13, 98. Crossref |
||||
Chopra, S. L., & Kanwar, J. S. (1980). Analytical Agricultural Chemistry. Kalyni Publishers, New Delhi. Pp. 337-346. | ||||
Cook, N. C., & Samman, S. (1996). "Review: flavonoids-chemistry, metabolism, cardioprotective effects and dietary sources. Journal of Nutritional Biochemistry, 7(2), 66-76. Crossref |
||||
Gulfraz, M., Kasuar, R., Arshad, G., Mehmood, S., Minhas, N., Asad, M. J., Ahmad, A., & Siddique, F. (2009). Isolation and characterization of edible oil from wild Olive. African Journal of Biotechnology, 8(16), 3734-3738. | ||||
Halliwell, B., & Gutterigde, J. M. C. (1984). Oxygen toxicity, oxygen radicals, transition metals and diseases. Biochemistry Journal, 219(1), 1-14. Crossref |
||||
Hussain, J., Khan, A. L., Rehman, N. U., Hamayun, M., Shah, T., Nisar, M., Bano, T., Shinwari, Z. K, & Lee, I. (2009). Proximate and nutrient analysis of selected vegetable species: A case study of Karak region, Pakistan. African Journal of Biotechnology; 8(12), 2725-2729. | ||||
James, C. S. (1995). Analytical Chemistry of Foods (1st ed.). Chapman and Hall, Glasgow. Crossref |
||||
Kengni, F., Tala, D. S., Djimeli, M. N., Fodouop, S. P. C., Kodjio, N., Magnifouet, H. N., & Gatsing, D. (2013). In vitro antimicrobial activity of Harungana madagascriensis and Euphorbia prostrata extracts against some pathogenic Salmonella. International Journal of Biological and Chemical Sciences, 7(3), 1106-1118. Crossref |
||||
Kumar, S. & Pandey, A. K. (2013). Phenolic content, reducing power and membrane protective activities of Solanum xanthocarpum root extracts. Vegetos, 26,301-307. Crossref |
||||
Kumaran, A., & Karunakaran, R. (2007). In vitro antioxidant activities of methanol extracts of Phyllanthus species from India. LWT-Food Science and Technology, 40, 344-352. Crossref |
||||
Leopoldini, M., Russo, N., Chiodo, S., & Toscano, M. (2006). Iron chelation by the powerful antioxidant flavonoid quercetin. Journal of Agricultural and Food Chemistry, 54(17), 6343-6351. Crossref |
||||
Malan D. F., Neuba, D. F. R., & Kouakou, K. L. (2015). Medicinal plants and traditional healing practices in Ehotile people, around the aby lagoon (eastern littoral of Côte d'Ivoire). Journal of Ethnobiology and Ethnomedicine, 11, 21. Crossref |
||||
Mba, J. R., Weyepe, F. C. L., Mokale, A. L. K, Tchamgoue A. D., Tchokouaha L.R.Y, Nole T, Tarkang P. A., Nehemie, D. T, Alembert, T. T, Mbita, M., Dongmo, B., & Agbor, G. A. (2017). Antidiarrhoeal, Antibacterial and Toxicological Evaluation of Harungana madagascariensis. Scholars Academic Journal of Biosciences, 5(3), 230-239. | ||||
Miliauskas, G., Venskutonis, P. R., & Van Beek, T. A. (2004). Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food chemistry, 85(2), 231-237. Crossref |
||||
Moulari, B., Pellequer, Y., Lboutounne, H., Girard, C., Chaumont, J. P., Millet, J., & Muyard, F. (2006). Isolation and in vitro antibacterial activity of astilbin, the bioactive flavanone from the leaves of Harungana madagascariensis Lam. ex Poir. (Hypericaceae). Journal of Ethnopharmacology, 106(2), 272-278. Crossref |
||||
Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxydant activity. Songklanakarin Journal of Science and Technology, 26(2), 211-219. | ||||
Ntie-Kang, F., Lifongo, L. L., Mbaze, L. M. A., Ekwelle, N., Owono, L. C. O., Megnassan, E., Judson, P. N., Sippl, W., & Efange, S. M. (2013). Cameroonian medicinal plants: a bioactivity versus ethnobotanical survey and chemotaxonomic classification. BMC Complementary and Alternative Medicine, 13, 147. Crossref |
||||
Rice-Evans, C. A, Miller, N. J., Bolwell P. G., Broamley, P. M., & Pridham, J. B. (1995). The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radical Research, 22(4), 375-383. Crossref |
||||
Ruffo, C. K., Birnie, A., & Tengnas, B. (2002). Edible Wild Plants of Tanzania. Regional Land management unit, Nairobi, Kenya. 776p. Retrieved 26th January, 2019 from. http://tropical.theferns.info/viewtropical.php?id=Harungana+madagascariensis. | ||||
Shorinwa, O. A., Mbajiorgu, A. C., & Afieroho, O. E. (2018). Trace element composition and anti-anaemic effects of the ethanol extract of Harungana madagascariensis Lam. Ex Poiret. in phenyl hydrazine induced anaemia in Albino rats. Journal of Pharmaceutical Research International, 21(2), 1-7. Crossref |
||||
Sies, H. (1985). Oxidative stress. Academic Press, San Diego. Pp. 1-8. Crossref |