RESEARCH JOURNAL OF FOOD SCIENCE AND NUTRITION
Integrity Research Journals

ISSN: 2536-7080
Model: Open Access/Peer Reviewed
DOI: 10.31248/RJFSN
Start Year: 2016
Email: rjfsn@integrityresjournals.org


Effects of processing methods on the chemical, anti-nutritional factors and functional properties of African yam bean (Sphenostylis stenocarpa) flour

https://doi.org/10.31248/RJFSN2026.227   |   Article Number: 0B5E3D754   |   Vol.11 (2) - April 2026

Received Date: 18 February 2026   |   Accepted Date: 30 March 2026  |   Published Date: 30 April 2026

Authors:  Ellen A. Beleya* , Georgina O. Anozie and Patricia E. Mbah

Keywords: flour, African Yam Bean, chemical composition, food processing.

The study assessed the effects of processing methods on the chemical, anti-nutritional factors and functional properties of African yam bean flour. The study was conducted at Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria. African yam bean seeds were processed into flour using different methods: cracked and boiled for 60 minutes; sprouted for 72 hours; toasted at 170℃ for 30 minutes; soaked for 12 hours and boiled for 30 minutes. Unprocessed African yam bean flour was used as the control. The chemical, anti-nutritional factors and functional properties of African yam bean flour were evaluated using standard methods of analysis. The results of the chemical composition of the flours revealed that the unprocessed African yam bean flour (UAF) had the highest contents of moisture, fat, crude fibre and amylopectin.  Sprouted African yam bean flour (SAF) had the highest contents of ash and crude protein. The soaked and boiled African yam bean flour (SBF) had the highest content of carbohydrate and amylose.  The results of anti-nutritional factors showed that there was a significant reduction in the oxalate, alkaloid, phytate and tannin contents of the processed African yam bean flour samples. The results of functional properties of the flour samples revealed that the bulk density, water absorption capacity and oil absorption capacity increased with processing, while the dispersibility reduced with processing. The study revealed that cracking and soaking prior to boiling were more effective in reducing the anti-nutritional factors and improved the ash, crude protein, fibre, amylose and the functional properties of the flour. African yam bean seeds should be subjected to different durations of soaking, boiling and sprouting to assess the best duration for improved nutrient content.

Abbas, Y., & Ahmed, A. (2018). Impact of processing on nutritional and antinutritional factors of legumes: A review. Annals of Food Science and Technology, 19(2), 199-215.
 
Abeshu, Y. & Kefale, B. (2017). Effect of some traditional processing methods on nutritional composition and alkaloid content of lupin bean. International Journal of Bioorganic Chemistry, 2(4), 174-179.
 
Abioye, V. F., Olatunde, S. J. & Elias, G. (2018). Quality attributes of cookies produced from composite flours of wheat, germinated finger millet flour and African yam bean. International Journal of Research-Granthaalayah, 6(11), 172-183.
https://doi.org/10.29121/granthaalayah.v6.i11.2018.1116
 
Adewale, B. D., & Nnamani, C. V. (2022). Introduction to food, feed and health wealth in African yam bean, a locked-in African indigenous tuberous legume. Frontiers in Sustainable Food Systems, 6, 726458
https://doi.org/10.3389/fsufs.2022.726458
 
Adeyanju, J. A., Babarinde, G. O., Olanipekun, B. F., Bolarinwa, I. F., & Oladokun, S. O. (2021). Quality assessment of flour and cookies from wheat, African yam bean and acha flours. Food Research, 5(1), 371-379.
https://doi.org/10.26656/fr.2017.5(1).370
 
Anih, D. C., Arowora, K. A., Boyi, R. N., Ugwuoke, K. C., Albukhari, A. F., Abah, M. A., Timothy, M., & Muhammad, Z. I. (2025). Effects of food processing on nutrient bioavailability and metabolic health. Asian Journal of Biological Sciences, 18(4), 885-897.
https://doi.org/10.3923/ajbs.2025.885.897
 
Anya, M. I., & Ozung, P. O. (2019). Proximate, mineral and anti-nutritional compositions of raw and processed African yam bean (Sphenostylis stenocarpa) seeds in Cross River State, Nigeria. Global Journal of Agricultural Sciences, 18, 19-29.
https://doi.org/10.4314/gjass.v18i1.3
 
Aondona, M. M., Orkuma, T. S., Leva, I. U. & Akpe, M. E. (2019). Effect of traditional processing methods on pesticide residue dissipation in cowpea (Vigna unguiculata). The International Journal of Engineering Science, 8(2), 28-36.
 
Arif, S., Ahmad, S., Masud, T., Khalid, N., Hayat, I., Siddique, F., & Muhammad, A. (2012). Effect of flour processing on the quality characteristics of a soy-based beverage. International Journal of Food Science and Nutrition, 63(8), 940-946.
https://doi.org/10.3109/09637486.2012.687365
 
Association of Official Analytical Chemists (AOAC) (2012). Official Methods of Analysis. 18th ed. AOAC Int., Gaithersburg, M.D. (Washington D.C., USA.).
 
Awuchi, C. G., Igwe, V. S., & Echeta, C. K. (2019). The functional properties of foods and flours. International Journal of Advanced Academic Research, 5(11), 139-160.
 
Azizah, A. H. & Zainon, H. (1997). Effect of processing on dietary fibre contents of selected legumes and cereals. Malaysian Journal of Nutrition, 3, 131-136.
 
Bala, K. S., & Rano, N. B. (2022). Influence of processing method on the proximate composition and antinutrient content of bambara nut (Vigna subterranea L.). Nigerian Journal of Animal Science and Technology, 5(2), 92-98.
 
Bodor, K., Szilagyi, J., Salamon, B., Szakacs, O., & Bodor, Z. (2024). Physical-chemical analysis of different types of flours available in the Romanian market. Scientific Reports, 14, 881.
https://doi.org/10.1038/s41598-023-49535-x
 
Chandra, S. & Samsher, S. (2013). Assessment of functional properties of different flours. African Journal of Agricultural Research, 8(38), 4849-4852.
 
Chauhan, D., Kumar, K., Ahmed, N., Thakur, P., Rizvi, Q. U. E. H., Jan, S., & Yadav, A. N. (2022). Impact of soaking, germination, fermentation and roasting treatments on nutritional, anti-nutritional and bioactive composition of black soybean (Glycine max L.). Journal of Applied Biology and Biotechnology, 10(5), 186-192.
https://doi.org/10.7324/JABB.2022.100523
 
Ekanayake, E. W. M. D. S., Navaratne, S. B., Wickramasinghe, I.. & Abeysundara, A. T. (2018). Determination of changes in amylose and amylopectin percentages in cowpea and green gram during storage. Nutrition and Food Science International Journal, 6(3), 555690.
https://doi.org/10.19080/NFSIJ.2018.06.555690
 
Eke-Ejiofor, J. & Beleya, E. A. (2017). Chemical, mineral pasting and sensory properties of spiced ogi (gruel). American Journal of Food Science and Technology, 5(5), 204-209.
https://doi.org/10.12691/ajfst-5-5-6
 
Eke-Ejiofor, J., Beleya, E. A. & Allen, J. E. (2022). Chemical and pasting properties of cassava-bambara groundnut flour blends. American Journal of Food Science and Technology, 10(2), 66-71.
 
Eke-Ejiofor, J., Beleya, E. A., & Onyenorah, N. I. (2014). The effect of processing methods on the functional and compositional properties of jackfruit flour. International Journal of Nutrition and Food Sciences, 3(3), 166-173.
https://doi.org/10.11648/j.ijnfs.20140303.15
 
El-Gohery, S. S. (2021). Effect of different treatments on nutritional value of lima bean (Phaseolus lunatus) and its utilisation in biscuit manufacture. Food and Nutrition Sciences, 12, 372-391.
https://doi.org/10.4236/fns.2021.124029
 
El-Hady, E. A. & Habiba, R. (2003). Effect of soaking and extrusion conditions on antinutrients and protein digestibility of legume seeds. LWT-Food Science and Technology, 36,285-293.
https://doi.org/10.1016/S0023-6438(02)00217-7
 
Giuberti, G., Tava, A., Mennella, G., Peccetti, L., Masoero, F., Sparvoli, F., Fiego, A. L. and Campion, B. (2019). Nutrients and antinutrients seed content in common bean (Phaseolus vulgaris L.) lines carrying mutations affecting seed composition. Agronomy, 9, 317.
https://doi.org/10.3390/agronomy9060317
 
Haji, A., Teka, T. A., Bereka, T. Y., Astatkie, T., Woldemariam, H. W., & Urugo, M. M. (2024). Effect of processing methods on the nutrient, antinutrient, functional, and antioxidant properties of pigeon pea (Cajanus cajan (L.) Millsp.) flour. Journal of Agriculture and Food Research, 18, 101493.
https://doi.org/10.1016/j.jafr.2024.101493
 
Han, L., Cao, S., Yu, Y., Xu, X., Cao, X., & Chen, W. (2021a). Modification in physicochemical, structural and digestive properties of pea starch during heat-moisture process assisted by pre- and post-treatment of ultrasound. Food Chemistry, 360(3), 129929.
https://doi.org/10.1016/j.foodchem.2021.129929
 
Henry-Unaeze, H. N., & Okoye, C. R. (2022). Evaluation of pasting and functional properties of flour blends made from African yam bean (Sphenostylis stenocarpa) and corn (Zea mays) seeds. International Journal of Innovative Science and Research, 7(3), 215- 221.
 
Huynh, D., Shrestha, A. K., & Arcot, J. (2016). Physicochemical properties and digestibility of eleven Vietnamese rice starches with varying amylose contents. Food and Function, 7, 3599-3608.
https://doi.org/10.1039/C6FO00661B
 
Ihemeje, A., Nwanekezi, E. C., Odimegwu, E. N., & Ekwe, C. C. (2018). Effect of processing methods of toasting, soaking, boiling, sprouting on dietary fibre and antinutrient contents of African yam bean and red kidney bean flour. European Journal of Food Science and Technology, 6(1), 40-48.
 
Inyang, U. E., Ibanga, U. I. & Enidiok, S. I. (2018). Changes in amino acid, anti-nutrients and functional properties of African yam bean flour caused by variation in steeping time prior to autoclaving. Asian Journal of Biotechnology and Bioresource Technology, 3(1), 1-10.
https://doi.org/10.9734/AJB2T/2018/39747
 
Iwe, M. O., Onyeukwu, U. & Agiriga, A. N. (2016). Proximate, functional and pasting properties of FARO 44 rice, African yam bean and brown cowpea seeds composite flour. Cogent Food and Agriculture, 2(1), 1142409.
https://doi.org/10.1080/23311932.2016.1142409
 
Kumari, P. V. & Sangeetha, N. (2017). Effect of processing and drying methods on the nutritional characteristic of the multi-cereal and legume flour. Journal of Food Processing and Technology, 8(4), 1000667.
 
Michel, M., Eldridge, A. L., Hartmann, C., Klassen, P., Ingram, J., & Meijer, G. W. (2024). Benefits and challenges of food processing in the context of food systems, value chains and sustainable development goals. Trends in Food Science & Technology, 153, 104703.
https://doi.org/10.1016/j.tifs.2024.104703
 
Mosisa, M. T. (2017). Effect of processing on proximate and mineral composition of black climbing (P. coccineus L.) bean flour. African Journal of Food Science, 11(3), 74-81.
https://doi.org/10.5897/AJFS2016.1475
 
Nnamani, C. V., Ajayi, S. A., Oselebe, H. O., Atkinson, C. J., Igboabuchi, A. N., & Ezigbo, E. C. (2017). Sphenostylis stenocarpa (ex. A. Rich.) Harms, a fading genetic resource in a changing climate: Prerequisite for conservation and sustainability. Plants, 6(30), 1-16.
https://doi.org/10.3390/plants6030030
 
Nwajagu, I. U., Garba, A., Nzelibe, H. C., Chukwukezie, N. E., Abah, C. R., Umar, A. T., Anarado, C. S., Kahu, J. C., Olagunju, A., Oladejo, A. A., & Bashiru, I. (2021). Effect of processing on the nutrient, anti-nutrient and functional properties of Mucuna flagellipes (Ox-eyed bean) seed flour; an underutilised legume in Nigeria. American Journal of Food and Nutrition, 9(1), 45-59.
 
Nwosu, J. N. (2013). Evaluation of the proximate and antinutritional properties of African yam bean (Sphenostylis stenocarpa) using malting treatment. International Journal of Basic and Applied Sciences, 2(4), 157-169.
 
Oboh, G., Ademiluyi, A. O. & Akindahunsi, A. A. (2010). The effect of roasting on the nutritional and antioxidant properties of yellow and white maize varieties. International Journal of Food Science and Technology, 45, 1236-1242.
https://doi.org/10.1111/j.1365-2621.2010.02263.x
 
Okoye, J. I., Igbokwe, Q. N., & Okechukwu, C. O. (2024). Effects of thermal processing on the nutritional and antinutritional properties of African yam bean (Sphenostylis stenocarpa) seed flours. Research Journal of Food Science and Nutrition, 9(2), 38-51.
https://doi.org/10.31248/RJFSN2024.167
 
Olaniyi, O. I., Adeniran, H. A. & Abiose, S. H. (2019). Antimicrobial characteristics of lactic acid bacteria in African yam bean-based drink. International Food Research Journal, 26(6), 1733-1740.
 
Olawumi, I., Osobie, C. C., Peter-Ikechukwu, A., Kabuo, O. N., Bede, N. E., & Odimegwu, N. (2014). Physicochemical and functional properties of flour from dehulled white African yam bean (Sphenostylis stenocarpa) grown in Anambra State, Nigeria. Natural Products an Indian Journal, 10(6), 171- 177.
 
Omeire, G. C. (2012). Amino acid profile of raw and extruded blends of African yam bean (Sphenostylis stenocarpa) and cassava flour. American Journal of Food Nutrition, 2, 65-68.
https://doi.org/10.5251/ajfn.2012.2.3.65.68
 
Onwuka, G.I. (2018). Food Analysis and Instrumentation: Theory and Practice. Second Edition, Naphtali Prints, Lagos, Nigeria.
 
Orhevba, B. A. & Aloysius, F. E. (2017). Effect of some processing methods on selected antinutritional factors of African breadfruit (Teculia Africana) seed flour. Futo Journal Series, 3(2), 65-70.
 
Popova, A. & Mihaylova, D. (2019). Antinutrients in plant-based foods: A review. The Open Biotechnology Journal, 13, 68-76.
https://doi.org/10.2174/1874070701913010068
 
Sobuola, F., Widodo, Y., & Kehinde, T. (2012). Processing and utilisation of legumes in the tropics. In: Eissa, A. A. (ed.), Trends in vital food and control engineering (pp. 71-84). Intech Open. Retrieved 5th September, 2021 from https://cdn.intechopen.com/pdfs/35126/intech-processing_and_utilization_of_legumes_in_the_tropics.pdf.
https://doi.org/10.5772/36496
 
Taiwo, K., Akanbi, C. & Ajibola, O. (1997). The effects of soaking and cooking time on the cooking properties of two cowpea varieties. Journal of Food Engineering, 33, 337-346.
https://doi.org/10.1016/S0260-8774(97)87278-0
 
Tura, D. C., Isho, A. A., Yetneberk, S., & Kebede, A. (2023). Effect of traditional processing methods on proximate composition and mineral content of Mung beans (Vigna radiata L.) grown in Ethiopia. Journal of Nutrition and Food Sciences, 13(5), 1-7.
 
Weaver, C. M., Dwyer, J., Fulgoni III, V. L., King, J. C., Leveille, G. A., MacDonald, R. S., Ordovas, J., & Schnakenberg, D. (2014). Processed foods: contributions to nutrition. The American Journal of Clinical Nutrition, 99(6), 1525-1542.
https://doi.org/10.3945/ajcn.114.089284
 
Yewande, B. A., & Thomas, A. O. (2015). Effects of processing methods on nutritive values of Ekuru from two cultivars of beans (Vigna unguiculata and Vigna angustifoliata). African Journal of Biotechnology, 14(21), 1790-1795.
https://doi.org/10.5897/AJB2014.14393