JOURNAL OF BIOSCIENCE AND BIOTECHNOLOGY DISCOVERY
Integrity Research Journals

ISSN: 2536-7064
Model: Open Access/Peer Reviewed
DOI: 10.31248/JBBD
Start Year: 2016
Email: jbbd@integrityresjournals.org


Biological control of pathogenic and secondary (non pathogenic) fungi associated with Barley (Hordeum vulgare) seeds

https://doi.org/10.31248/JBBD2017.028   |   Article Number: CDDFEFEB1   |   Vol.2 (2) - April 2017

Received Date: 06 March 2017   |   Accepted Date: 29 March 2017  |   Published Date: 30 April 2017

Author:  Ihsan Flayyih Hasan AI-Jawhari

Keywords: bacteria, Antagonism, pollution., phytopathogenic, competition, fungicides.

Plant pathogenic fungi are a major problem in agriculture with effects on yield and quality of agricultural product. In this study, antagonistic effects of five fungi biocontrol agent Aspergillus niger, Aspergillus ostianus, Aspergillus versicolor, Penicillium sp., Trichoderma harzianum and one species of bacterium Pseudomonas aeruginosa isolated from the seeds of barley were evaluated against plant pathogenic fungi Rhizoctonia solani and secondary (non-pathogenic) fungi Aspergillus flavus. The ability of isolated microorganisms in antagonizing or inhibiting the growth of phytopathogenic fungi was tested by measuring the growth inhibition percentage over control. P. aeruginosa obtained higher inhibition with R. solani and A. flavus (88%, 76%) respectively, but A. ostianus obtained the lower inhibition with R. solani and A. flavus (59%, 53%) respectively. The results also showed that significant differences were recorded in mycelial growth of pathogens in presence of biocontrol agent when compared with control. Also, the results showed that non-significant differences were recorded between R. solani and A. flavus when treated with different antagonisms.

Agrios, G. N. (2005). Plant pathology, 5th ed. Elsevier Academic Press, p. 952.
 
Bergy's, N. R., & Holt, J. G. (1994). Bergy'smanual of systematic bacteriology, 9th (ed). Williams and Wilkins Baltimore, p. 756.
 
Bruce, A., Srinivasan, U., & Staines, H. J., & Highly, T. L. (1995). Chitinase and laminarinase production in liquid culture by Trichoderma spp. and their role in biocontrol of wood decay fungi. International Biodeterioration&Biodegradation, 35(4), 337- 353.
Crossref
 
Canessa, A. A., & Morrell, J. J. (1996). Effect of Trichoderma harzianumon induction of laccase by Trametes versicolor on Ponderosapinsap wood. Document International Research Group on wood preservation. Stockholm IRG Secretariat.
 
Chet, I., Inbar, J., & Hadar, I. (1997). Fungal antagonists and mycoparasites. In: Wicklow, D. T., & Soderstrom,B. (ed), The mycota IV: Environmental and microbial relationships . Springer – Verlag, Berlin, Pp. 165-184.
 
Christy Jeyaseelan, E., Tharmila, S., & Niranjan, K. (2012). Antagonisitic activity of Trichoderma spp. and Bacillus Spp. Against Pythium aphanidermatum isolated from tomoto damping off. Archives of Applied Sience Research, 4(4), 1623-1627.
 
Egorov, N. S. (1985). Antibiotic: a scientific approach. Mir Publishers, Moscow.
 
Harman, G. E. (2000). Myths and dogmas of biocontrol. Plant disease .84(4), 377-393.
Crossref
 
Harman, G. E., Backman, P. A., & Tumer, J. T. (1989). Symposium bio-control and biotechnological methods for controlling cotton pets. En: Brown JM, Richer DA (Eds) Proceedings of the Bel-twide cotton production research conference. Memphis, Tennessee. USA. Pp. 15-20.
 
Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J., & Willams, S. T. (1994). Bergy's manual of determination bacteriology. 9th edition, Williams and Wikins . Baltimore, Maryland, p. 787.
 
Horvath, E. M., Burgel, J. L., & Messner, K. (1995). The Production of soluble antifungal metabolites by the biocontrol fungus Trichoderma harzianumin connection with the formation of conidiospore. Material and organisms, 29, 1- 14.
 
Ikotum, T., Agboola, O. H. (1992). In vitro using the microorganisms in inhibition of pathogenic fungi growth. Egyptian Journal of Microbiology, 24(3), 415-421.
 
Kucuk, C., & Kivanc, M. (2004). In vitro antifungal activity of strains of Trichoderma harzianum. Turkey Journal of Biology, 28, 111-115.
 
La Penna, M., Nesci, A., &Etcheverry, M. (2004). In vitro studies on the potential for biological control on Aspergillus section Flavi by Kluyveromyces spp. Letters in Applied Microbiology.38, 257-264.
Crossref
 
Lartey, R. T., Curl, E. A., & Peterson, C. M. (1994). Interactions of mycophagous collembolan and biological control fungi in the suppression of Rhizoctonia solani. Soil Biology and Biochemistry, 26(1), 1-88.
Crossref
 
Latunde, A. O. (1991). The use of Trichoderma koningiiin the cotton of web blight disease caused by Rhizoctonia solani in the foliage of cowpea (Vignaun guiculata). Journal of Phytopathology, 133(3), 247-254.
Crossref
 
Lewis, J. A., &Papavizas, G. C. (1991). Biocontrol to plant diseases. The approach for tomorrow. Crop Protection. 10, 95-105.
Crossref
 
Marchetti, R., Nipoti, P., Ercole, N. D., & Guerzoni, M. E. (1992). Competition at atmospherice level as biocontrol mechanism in Tricoderma spp. Petria, 2, 137-147.
 
Matar, S. M., EI-Kazzar, S. A., Wagih, E. E., EI-Diwany, A. I., Moustafa, H. E., Abo-Zaid, G. A., Abd-EIsalam, Hafez, E. E. (2009). Antagonistic and inhibitory effect of Bacillus subtilis against certain plant pathogenic fungi. International Biotechnology, 8, 53-61.
Crossref
 
Moita, C., Feio, S. S., Nunes, L., Curto, M. J. M., &Roseiro, J. C. (2005). Optimisation of physical factors on the production of active metabolites by Bacillus subtilis 355 against wood surface contaminat fungi. International Biodeterioration & Biodegradation, 55(4), 261-269.
Crossref
 
Monte, E. (2001). Understanding Trichoderma: between biotechnology and microbial ecology. International Journal of Microbiology .4, 1- 4.
 
Nourozian, J., Etebarian, H. R., & Khodakaramian, G. (2006). Biological control of Fusarium graminearum on wheat by antagonistic bacteria. Songklanakarin Journal Science and Technology, 28(1), 29- 38.
 
Reddy, K. R. (2010). Microbiology, parasitology. Question Answer review. 4th ed, PARAS. Medical publisher, Pp. 58-189.
 
Santamarina., M. P., Josefa, R., Reyes, L., & Vicent, S. (2002). Antagonistic activity of Penicillium oxalicum corrie and Thom, Penicillium decumbens Thom and Trichoderma harzianum Rifai isolated against fungi, bacteria and insects in vitro. Review Iberoam Micobiology .19(2), 99-103.
 
Shaikh Farah, T., & Sahera, N. (2016). Antifungal activity of Pseudomnas aeruginosa and Bacillus subtilis against pathogens of Cucurbita ceousfruits. International Journal of Innovative Research in Science, Engineering and Technology, 5(3), 3320-3324.
 
Siddiqui, S., Siddiqui, Z. A., & Ahmad, I. (2005). Evaluation of fluorescent Pseudomonas and Bacillus isolates for the biocontrol of a wilt disease complex of pigeon pea. World Journal of Microbiology and Biotechnology, 21(5), 729-732.
Crossref
 
Tharmila, S., Vasanthakala, R., Nalini, S., & Arulanantham, C. T. (2013). In vitro screening of antagonistic effect of soil born bacteria on some selected phytopathogenic fungi. Archives of Applied Science Research. 5(1), 1- 4.
 
Vey, A., Hoagland, R. E., & Butt, T. M. (2001). Toxic metabolites of fungal biocontrol agents fungi as Biocontrol Agent. Progress, problem sand potential (Butt, T. M., Jackson & Magan, N, eds), CABI Publishing, Oxford, UK. Pp. 311-346.
 
Vincent, J. M. (1927). Distribution of fungal hyphae in presence of certain inhibitors. Nature. 59, 850.
 
Watanabe, T. (2002). Pictorial atlas of soil and seed fungi: morphologies of cultured fungi and key to species. 2nd edition.India.CRC Press. p. 504.
Crossref