ISSN: 2536-7064
Model: Open Access/Peer Reviewed
DOI: 10.31248/JBBD
Start Year: 2016
Email: jbbd@integrityresjournals.org
https://doi.org/10.31248/JBBD2021.159 | Article Number: B70DBE9D2 | Vol.6 (5) - December 2021
Received Date: 02 October 2021 | Accepted Date: 18 November 2021 | Published Date: 30 December 2021
Authors: Ihuoma Ahaotu , Marvis Wondikom and Ndukwe Maduka*
Keywords: spices, Bacillus species, endospores, foodborne disease, Zingiber officinale.
The formation of spores and increase in population of vegetative cells of Bacillus cereus associated with foodborne diseases is dependent on favourable temperature. In this study, B. cereus in a slant obtained from Microbiology Laboratory, University of Port Harcourt was confirmed based on morphological and biochemical tests and inoculated into dry ginger powder using a sterile pipette. The samples were stored at ambient temperature (25°C), refrigeration temperature (4°C) and freezer temperature (-18°C) for 21 days and the microbial counts of B. cereus was monitored at 7 days interval using Bacillus cereus agar base and standard microbiological methods. The result obtained showed that B. cereus steadily increased in population under the storage conditions. The highest microbial count recorded for B. cereus in dry ginger powder stored at 25°C, 4°C and -18°C was 7.15×101 ±0.036, 1.07×102 ± 0.061 and 2.91×102 ± 0.070 CFU/g, respectively. Although the values did not exceed 105 cells/g required to manifest foodborne illness in humans, it remains a risk to public health due to increasing population of B. cereus in dry ginger powder under the storage conditions.
Aksu, H., Bostan, K., & Erugün, Ö. (2000). Presence of Bacillus cereus in packaged some spices and herbs sold in Istanbul. Pakistan Journal of Biological Sciences, 3(5), 710-712. Crossref |
||||
Akter, M., Bithi, N., Billah, M., Mustak, S., & Rashid, M. (2020). Evaluation of nutritional and mineral content of dehydrated ginger (Zingiber officinale). European Journal of Medicinal Plants, 31(17), 21-28. Crossref |
||||
Al-Awwadi, N. A. J. (2017). Potential health benefits and scientific review of ginger. Journal of Pharmacognosy and Phytotherapy, 9(7), 111-116. Crossref |
||||
Bae, Y., Kim, B., Lee, S., Cha, M., Park, K., Chung, M., & Ryu, K. (2012). Growth and predictive model of Bacillus cereus on blanched spinach with or without seasoning at various temperatures. Food Science and Biotechnology, 21(2), 503-508. Crossref |
||||
Bag, B. B. (2018). Ginger processing in India (Zingiber officinale): a review. International Journal of Current Microbiology and Applied Sciences, 7(4), 1639-1651. Crossref |
||||
Berthold-Pluta, A., Pluta, A., Garbowska, M., & Stefańska, I. (2019). Prevalence and toxicity characterization of Bacillus cereus in food products from Poland. Foods, 8(7), 269. Crossref |
||||
Bhatt, N., Waly, M. I., Essa, M. M., & Ali, A. (2013). Ginger: A functional herb. Food as Medicine, Pp. 51-71. | ||||
Doren, J. M. V., Neil, K. P., Parish, M., Gieraltowski, L., Gould, L. H., & Gombas, K. L. (2013). Foodborne illness outbreaks from microbial contaminants in spices, 1973-2010. Food Microbiology, 36(2), 456-464. Crossref |
||||
Duport, C., Jobin, M., & Schmitt, P. (2016). Adaptation in Bacillus cereus: from stress to disease. Frontiers in Microbiology, 7, 1550. Crossref |
||||
Feijoo, S. C., Cotton, L. N., Watson, C. E., & Martin, J. H. (1997). Effect of storage temperatures and ingredients on growth of Bacillus cereus in coffee creamers. Journal of Dairy Science, 80(8), 1546-1553. Crossref |
||||
Fogele, B., Granta, R., Valciņa, O., & Bērziņš, A. (2018). Occurrence and diversity of Bacillus cereus and moulds in spices and herbs. Food Control, 83, 69-74. Crossref |
||||
Guirguis, E. A. H. (2020). Comparative study of ginger (Zingiber officinale Roscoe) as raw an herbal tea: microbiological, mycotoxin, and phytochemical quality. Journal of Medicine in Scientific Research, 3(1), 12-17. | ||||
Kwon, M. J., Lee, C. L., & Yoon, K. S. (2019). Risk comparison of the diarrheal and emetic type of Bacillus cereus in tofu. Microorganisms, 7(11), 536. Crossref |
||||
Mathot, A. G., Postollec, F., & Leguerinel, I. (2021). Bacterial spores in spices and dried herbs: The risks for processed food. Comprehensive Reviews in Food Science and Food Safety, 20, 840-862. Crossref |
||||
Mendi, S., Nain, C., Imélé, H., Ngoko, Z., & Mbofung, M. F. (2009). Microflora of fresh ginger rhizomes and ginger powder produced in the North-West region of Cameroon. Journal of Animal and Plant Sciences, 4(1), 251-260. | ||||
Moore, R. E., Millar, B. C., Panickar, J. R., & Moore, J. E. (2019). Microbiological safety of spices and their interaction with antibiotics: implications for antimicrobial resistance and their role as potential antibiotic adjuncts. Food Quality and Safety, 3, 93-97. Crossref |
||||
Okanlawon, B. M., Ogunbanwo, S. T., & Okunlola, A. O. (2010). Growth of Bacillus cereus isolated from some traditional condiments under different regimens. African Journal of Biotechnology, 8(14), 2129-2135. | ||||
Ölmez, H. K., & Aran, N. (2005). Modeling the growth kinetics of Bacillus cereus as a function of temperature, pH, sodium lactate and sodium chloride concentrations. International Journal of Food Microbiology, 98, 135-143. Crossref |
||||
Pexara, A., & Govaris, A. (2010). Bacillus cereus: an important foodborne pathogen. Journal of the Hellenic Veterinary Medical Society, 61(2), 127-133. Crossref |
||||
Senay, T. L. (2020). Systematic review on spices and herbs used in food industry. American Journal of Ethnomedicine, 7(20), 1-10. | ||||
Shahrajabian, M. H., Sun, W., & Cheng, Q. (2019). Clinical aspects and health benefits of ginger (Zingiber officinale) in both traditional Chinese medicine and modern industry. Acta Agriculturae Scandinavica, Section b-Soil & Plant Science, 69(6), 546-556. Crossref |
||||
Shoaib, M., Muzammil, I., Hammad, M., Bhutta, Z. A., & Yaseen, I. (2020). A mini-review on commonly used biochemical tests for identification of bacteria. International Journal of Research Publications, 54(1), 1-7. Crossref |
||||
Singh, P., Srivastava, S., Singh, V. B., Sharma, P., & Singh, D. (2018). Ginger (Zingiber officinale): a nobel herbal remedy. International Journal of Current Microbiology and Applied Sciences, 7, 4065-4077. | ||||
Tewari, A., & Abdullah, S. (2015). Bacillus cereus food poisoning: international and Indian perspective. Journal of Food Science and Technology, 52(5), 2500-2511. Crossref |
||||
Ubong, A., New, C. Y., Chai, L. C., Nur Fatihah, A., Nur Hasria, K., Nishibuchi, M., & Son, R. (2019). Impact of temperature on Bacillus cereus spore germination in ultra-high temperature chocolate milk. Food Research, 3(6), 808-813. Crossref |
||||
Ugwoke, C. E. C., & Nzekwe, U. (2010). Phytochemistry and proximate composition of ginger (Zingiber officinale). Journal of Pharmaceutical and Allied Sciences, 7(5), 1182-1187. Crossref |