JOURNAL OF BIOSCIENCE AND BIOTECHNOLOGY DISCOVERY
Integrity Research Journals
ISSN: 2536-7064
Model: Open Access/Peer Reviewed
DOI: 10.31248/JBBD
Start Year: 2016
Email: jbbd@integrityresjournals.org
A preliminary study on the effect of storage temperatures on the population of Bacillus cereus in dry ginger powder
https://doi.org/10.31248/JBBD2021.159 | Article Number: B70DBE9D2 | Vol.6 (5) - December 2021
Received Date: 02 October 2021 | Accepted Date: 18 November 2021 | Published Date: 30 December 2021
Authors: Ihuoma Ahaotu , Marvis Wondikom and Ndukwe Maduka*
Keywords: spices, Bacillus species, endospores, foodborne disease, Zingiber officinale.
The formation of spores and increase in population of vegetative cells of Bacillus cereus associated with foodborne diseases is dependent on favourable temperature. In this study, B. cereus in a slant obtained from Microbiology Laboratory, University of Port Harcourt was confirmed based on morphological and biochemical tests and inoculated into dry ginger powder using a sterile pipette. The samples were stored at ambient temperature (25°C), refrigeration temperature (4°C) and freezer temperature (-18°C) for 21 days and the microbial counts of B. cereus was monitored at 7 days interval using Bacillus cereus agar base and standard microbiological methods. The result obtained showed that B. cereus steadily increased in population under the storage conditions. The highest microbial count recorded for B. cereus in dry ginger powder stored at 25°C, 4°C and -18°C was 7.15×101 ±0.036, 1.07×102 ± 0.061 and 2.91×102 ± 0.070 CFU/g, respectively. Although the values did not exceed 105 cells/g required to manifest foodborne illness in humans, it remains a risk to public health due to increasing population of B. cereus in dry ginger powder under the storage conditions.
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