JOURNAL OF BIOSCIENCE AND BIOTECHNOLOGY DISCOVERY
Integrity Research Journals
ISSN: 2536-7064
Model: Open Access/Peer Reviewed
DOI: 10.31248/JBBD
Start Year: 2016
Email: jbbd@integrityresjournals.org
Prevalence of trypanosome species in wild Tsetse flies (Glossina spp) from Kagarko forest, North-Central Nigeria
https://doi.org/10.31248/JBBD2024.208 | Article Number: D581F3411 | Vol.9 (2) - June 2024
Received Date: 28 March 2024 | Accepted Date: 25 April 2024 | Published Date: 30 June 2024
Authors: Attahir, A.* , Usman B. M. , Isadu H. T. , Imam, I. U. , Jabiru G. , Idris B. M. , Anchau, R. G. , Ramatu, A. A. , Yusuf, A. B. , Rabiu, A. I. , Shettima F. A. T. , Karma, A. B. and Hudu B.E.
Keywords: infection., African Trypanosomiasis, Kagarko forest, Trypanosoma vivax.
The causative agents of Human African Trypanosomiasis (HAT) are trypanosome parasites belonging to the trypanosoma brucei complex: T. brucei gambiense is responsible for the chronic form of the disease in West and Central Africa; T. brucei rhodesiense is responsible for the acute form in East and Southern Africa; While T. brucei brucei is used as an animal model for the HAT. Other trypanosomes responsible for the disease in animals called Animal African Trypanosomiasis (AAT) includes: Trypanosoma congolense, Trypanosoma vivax and Trypanosoma evansi. In this study, the prevalence and identity of trypanosome species in wild tsetse flies from Kagarko forest was investigated by nested PCR using ITS-1 generic and species-specific primers. Five hundred and eight-six flies were trapped using bi-conical traps and identified by standard morphological features. DNA extracted from whole flies was used for nested PCR to amplify the ITS-1 region of trypanosomal rDNA. Two tsetse fly species were identified in the Kagarko forest: Glossina tachinoides (480) and Glossina palpalis palpalis (106), representing 81.91% and 18.09% respectively. While the predominant trypanosome spp found were Trypanosoma vivax (38.2%), Trypanosoma brucei (30.9%) and Trypanosoma congolense Savanna (21.8%). These results revealed high prevalence of trypanosome infections in wild tsetse flies in Kagarko forest which has important impacts on the health and socio-economic wellbeing of the people and animals in communities around the forest.
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