JOURNAL OF BIOSCIENCE AND BIOTECHNOLOGY DISCOVERY
Integrity Research Journals

ISSN: 2536-7064
Model: Open Access/Peer Reviewed
DOI: 10.31248/JBBD
Start Year: 2016
Email: jbbd@integrityresjournals.org


Growth performance and nitrogen utilisation of grower-finisher pigs fed amino acid supplementation of low crude protein diets

https://doi.org/10.31248/JBBD2025.232   |   Article Number: AB77916F1   |   Vol.10 (3) - August 2025

Received Date: 16 March 2025   |   Accepted Date: 07 May 2025  |   Published Date: 30 August 2025

Authors:  Folorunso, O. T. , Adeola, A. B. , Fasuyi, A. O. and Fajemilehin, S. O. K.*

Keywords: growth performance, nitrogen utilisation, Amino Acid supplementation, low crude protein diets, sustainable swine production.

This study evaluated the effect of low crude protein (CP) diets supplemented with essential amino acids (AAs) on the growth performance and nitrogen (N) utilisation of grower-finisher pigs. Sixteen pigs were randomly assigned to four dietary treatments: a control diet (18% CP) and three low-CP diets (15, 12, and 9% CP) supplemented with lysine, methionine, threonine, and tryptophan. The diets were formulated to be isoenergetic, and the experiment was conducted at the Teaching and Research Farm, Ekiti State University, Nigeria, under controlled conditions. Growth performance parameters, including average daily weight gain (ADWG), average daily feed intake (ADFI), and feed conversion ratio (FCR), were significantly (p<0.05) influenced by dietary treatments. Pigs fed with the 12% CP diet (Diet 3) exhibited the highest ADWG (539.90 ± 10.10 g) and the lowest FCR (2.12 ± 13.11), while those on the 9% CP diet (Diet 4) had the least ADWG (391.25 ± 50.52 g) and the highest FCR (2.8 ± 58.58). Nitrogen utilisation was also significantly (P<0.05) affected, with the highest N retention observed in the control diet (29.69 ± 3.52 g/day), followed by the 15% CP diet (28.92 ± 1.69 g/day). Urinary N excretion was highest in the control diet (6.29 ± 0.47 g/day) and lowest in the 12% CP diet (3.57 ± 0.52 g/day). These findings suggest that reducing dietary CP to 12% with AA supplementation maintains optimal growth performance and improves N utilisation efficiency, while further reduction to 9% CP impairs performance. However, excessive CP reduction, as seen in Diet 4, may limit AA availability, leading to suboptimal performance. This study highlights the potential of low-CP diets with AA supplementation to enhance sustainable swine production by improving N utilisation and reducing environmental impact.

Almeida, A. M., Latorre, M. A., Ripoll, G., Verdú, M., & Alvarez-Rodriguez, J. (2024). Reducing dietary protein levels with amino acid supplementation in Duroc sire line finishing pigs: Growth performances, carcass and meat traits, and nitrogen balance in males and females. Animals, 14(24), 3572.
https://doi.org/10.3390/ani14243572
 
Cai, Y. C., Chen, S. C., Li, J. S., Lu, X. L., Lu, S. Y., Li, H. X., & Zhang, Y. (2024). Effects of crude protein levels on growth performance, nitrogen balance, digestibility, and crude protein requirement of growing Fuxing pigs. Journal of Animal & Plant Sciences, 34(2), 318-324.
https://doi.org/10.36899/JAPS.2024.2.0719
 
Cappelaere, L., Le Cour Grandmaison, J., Martin, N., & Lambert, W. (2021). Amino acid supplementation to reduce environmental impacts of broiler and pig production: A review. Frontiers in Veterinary Science, 8, Article 689259.
https://doi.org/10.3389/fvets.2021.689259
 
Carpenter, D. A., O'Mara, F. P., & O'Doherty, J. V. (2004). The effect of dietary crude protein concentration on growth performance, carcass composition, and nitrogen excretion in entire grower-finisher pigs. Irish Journal of Agricultural and Food Research, 43, 227-236.
 
Cho, I., An, S. H., Yoon, J. H., Namgung, N., & Kong, C. (2024). Growth performance and nitrogen excretion of broiler chickens fed low protein diets supplemented with crystalline amino acids. Journal of Animal Science and Technology, 66(1), 145-155.
https://doi.org/10.5187/jast.2023.e131
 
Duarte, M. E., Parnsen, W., Zhang, S., Abreu, M. L. T., & Kim, S. W. (2024). Low crude protein formulation with supplemental amino acids for its impacts on intestinal health and growth performance of growing-finishing pigs. Journal of Animal Science and Biotechnology, 15, Article 55.
https://doi.org/10.1186/s40104-024-01015-6
 
Esteves, L. A. C., Monteiro, A. N. T. R., Sitanaka, N. Y., Oliveira, P. C., Castilha, L. D., Paula, V. R. C., & Pozza, P. C. (2021). The reduction of crude protein with the supplementation of amino acids in the diet reduces the environmental impact of growing pigs production evaluated through life cycle assessment. Sustainability, 13(9), Article 4815.
https://doi.org/10.3390/su13094815
 
Gloaguen, M., Le Floc'h, N., Corrent, E., Primot, Y., and van Milgen, J. (2014). The use of free amino acids allows formulating very low crude protein diets for piglets. Journal of Animal Science, 92(2), 637-644.
https://doi.org/10.2527/jas.2013-6514
 
Greenhalgh, S., Chrystal, P. V., Selle, P. H., & Liu, S. Y. (2020). Reduced-crude protein diets in chicken-meat production: Justification for an imperative. World's Poultry Science Journal, 76(3), 456-472.
https://doi.org/10.1080/00439339.2020.1789024
 
Kerr, B. J., Weber, T. E., and Shurson, G. C. (2017). Balancing diets for amino acids and energy to maximize nitrogen retention in pigs. Journal of Animal Science and Biotechnology, 8(1), 1-10.
 
Le Bellego, L., van Milgen, J., and Noblet, J. (2001). Effect of high temperature and low-protein diets on the performance of growing-finishing pigs. Journal of Animal Science, 79(5), 1849-1857.
https://doi.org/10.2527/2001.7951259x
 
Li, P., Zhang, Y., Li, X., and Han, J. (2018). Effects of low-protein diets supplemented with essential amino acids on growth performance and nitrogen excretion in pigs. Animal Nutrition, 4(2), 156-161.
 
Liu, S. Y., Macelline, S. P., Chrystal, P. V., & Selle, P. H. (2021). Progress towards reduced-crude protein diets for broiler chickens and sustainable chicken-meat production. Journal of Animal Science and Biotechnology, 12, Article 20.
https://doi.org/10.1186/s40104-021-00550-w
 
Niyonsaba, A., Jin, X. H., & Kim, Y. Y. (2023). Effect of reducing dietary crude protein level on growth performance, blood profiles, nutrient digestibility, carcass traits, and odour emissions in growing-finishing pigs. Animal Bioscience, 36(10), 1584-1595.
https://doi.org/10.5713/ab.23.0155
 
NRC (2012). Nutrient Requirements of Swine (11th ed.). National Academies Press.
 
Portejoie, S., Dourmad, J. Y., Martinez, J., and Lebreton, Y. (2004). Effect of lowering dietary crude protein on nitrogen excretion, manure composition, and ammonia emission from fattening pigs. Livestock Production Science, 91(1-2), 45-55.
https://doi.org/10.1016/j.livprodsci.2004.06.013
 
Patráš, P., Nitrayová, S., Brestenský, M., & Heger, J. (2009). Effect of dietary fibre and dietary protein level on nitrogen excretion pattern of growing pigs. Archiva Zootechnica 12:3, 5-10
 
Rocha, G. C., Duarte, M. E., & Kim, S. W. (2022). Advances, implications, and limitations of low crude-protein diets in pig production. Animals, 12(24), 3478.
https://doi.org/10.3390/ani12243478
 
Wang, X., Wu, W., & Li, Z. (2019). Effects of low-protein diets supplemented with crystalline amino acids on growth performance and nitrogen utilisation in pigs. Journal of Animal Physiology and Animal Nutrition, 103(2), 567-575.
 
Wang, Y., Han, S., Zhou, J., Li, P., Wang, G., Yu, H., Cai, S., Zeng, X., Johnston, L. J., Levesque, C. L., and Qiao, S. (2020). Effects of dietary crude protein level and N-carbamylglutamate supplementation on nutrient digestibility and digestive enzyme activity of jejunum in growing pigs. Journal of Animal Science, 98(4), skaa088.
https://doi.org/10.1093/jas/skaa088
 
Zhang, S., Qiao, S., Ren, M., Zeng, X., Ma, X., Wu, Z., and Thacker, P. (2013). Supplementation with branched-chain amino acids to a low-protein diet regulates intestinal expression of amino acid and peptide transporters in weanling pigs. Amino Acids, 45(5), 1191-1205.
https://doi.org/10.1007/s00726-013-1577-y
 
Zhao, Y., Tian, G., and Chen, D. (2019). Effect of different dietary protein levels and amino acids supplementation patterns on growth performance, carcass characteristics and nitrogen excretion in growing-finishing pigs. Journal of Animal Science and Biotechnology, 10, 75.
https://doi.org/10.1186/s40104-019-0381-2