JOURNAL OF BIOSCIENCE AND BIOTECHNOLOGY DISCOVERY
Integrity Research Journals
ISSN: 2536-7064
Model: Open Access/Peer Reviewed
DOI: 10.31248/JBBD
Start Year: 2016
Email: jbbd@integrityresjournals.org
Relationship between prolactin genetic polymorphisms and growth performance trait in Nigerian indigenous chickens
https://doi.org/10.31248/JBBD2025.226 | Article Number: 8B1109431 | Vol.10 (1) - April 2025
Received Date: 27 January 2025 | Accepted Date: 15 March 2025 | Published Date: 30 April 2025
Authors: Ige, A. O. , Hammmed, O. O. , Ajibulu, J. O. , Salawu, H. O. , Matt-Obabu, A. D.* , Akinniran, K. O. and Gbadebo, O. J.
Keywords: growth, indigenous, gene, prolactin, polymorphisms
The two experiments were conducted to determine the qualitative trait between Normal and Frizzle feathered chickens. The second experiment compares the genotypic and allelic frequency of prolactin gene polymorphism in Normal (NF) and Frizzle feathered (FF) chickens. Plumage colour is a notable qualitative trait in the Nigerian indigenous chickens. Three plumage colour were observed through visual appraisal which are multi-coloured, white and black. Blood was collected and released on a Fast Technology Analysis (FTA) paper and was taken to a laboratory. The results of the present study showed the presence of polymorphism for the prolactin (PRL) gene which shows two alleles and genotype; I, D and ID respectively in FF chicken and two alleles (I and D) and three genotypes (II, DD and ID) in NF chicken. Frequency and percentage of each colour were carried out. The frequency and percentage of each colour in the NF chicken is multi-coloured (48 and 92.31%), white (3 and 5.77%) and black (1 and 5.77%) respectively. No appearance of white colour was seen in FF chicken, however, the frequency and percentage of black is (1 and 1.25%) and multi-coloured (7 and 87.5%) respectively. Three genotypes were observed including II, ID and DD which consequently have two alleles I and D during gel electrophoresis using agarose gel after specific primer have been amplified using PCR machine with specific temperature degree needed by manual/direct counting. Conclusively, prolactin gene can be used as marker in the genetic improvement of Nigerian indigenous chickens due to the presence of polymorphism.
| Akpan, U., Olowookere, V. O., & Ikeobi, C. O. N. (2025). Relationship between pituitary specific transcription factor-1 (pit-1) genotypic variants and non-linear growth curve parameters in Funaab alpha chickens. Slovak Journal of Animal Science, 58(1), 3-12. https://doi.org/10.36547/sjas.930 |
||||
| Angelier, F. (2024). Hormones and regulation of parental behavior in birds. In Hormones and Reproduction of Vertebrates, Volume 4 (pp. 207-240). Academic Press. https://doi.org/10.1016/B978-0-443-16024-0.00003-4 |
||||
| Fakhr, A. E., Baioumy, S. A., & Mohamed, R. A. (2024). Association of toxoplasma infection and susceptibility with NLRP1 rs8081261 and rs11652907 gene polymorphism using RFLP among Egyptian population. Microbes and Infectious Diseases, 5(4), 1667-1677. https://doi.org/10.21608/mid.2024.304710.2081 |
||||
| Gao, G., Zhang, H., Ni, J., Zhao, X., Zhang, K., Wang, J., Kong, X., & Wang, Q. (2023). Insights into genetic diversity and phenotypic variations in domestic geese through comprehensive population and pan-genome analysis. Journal of Animal Science and Biotechnology, 14(1), 150. https://doi.org/10.1186/s40104-023-00944-y |
||||
| Goli, R. C., Sukhija, N., Rathi, P., Chishi, K. G., Koloi, S., Malik, A. A., Purohit, P. B., Shetkar, M., & KK, K. (2024). Unraveling the genetic tapestry of Indian chicken: A comprehensive study of molecular variations and diversity. Ecological Genetics and Genomics, 30, 100220. https://doi.org/10.1016/j.egg.2024.100220 |
||||
| Google Earth Map (2024). Geographical location of LAUTECH, Ogbomoso, Oyo State, Nigeria. Google LLC. Retrieved October 25, 2024 from http://earth.google.com/web/ search/lautech+ogbomoso. | ||||
| Hoda, A., Berisha, B., Bixheku, X., & Zanchi, F. B. (2024). Comprehensive in silico analysis of prolactin receptor (PRLR) gene nonsynonymous single nucleotide polymorphisms (nsSNPs) reveals multifaceted impact on protein structure, function, and interactions. Journal of Biomolecular Structure and Dynamics, 1-17. https://doi.org/10.1080/07391102.2024.2335295 |
||||
| Huang, B., Khan, M. Z., Chai, W., Ullah, Q., & Wang, C. (2023). Exploring genetic markers: mitochondrial DNA and genomic screening for biodiversity and production traits in donkeys. Animals, 13(17), 2725. https://doi.org/10.3390/ani13172725 |
||||
| Kar, B., Bahar, O. Z., Yazdic, F. C., & Karaman, A. (2024). Effects of mutation in myostatin exon 3 on growth and carcass characteristics of Savak Akkaraman sheep. Small Ruminant Research, 241, 107386. https://doi.org/10.1016/j.smallrumres.2024.107386 |
||||
| Kaur, S., Vashistt, J., Sharma, A., Parkash, J., Kumar, A., Duseja, A., & Changotra, H. (2024). Mutagenic primer-based novel multiplex PCR-RFLP technique to genotype BECN1 SNPs rs10512488 and rs11552192. Molecular Biology Reports, 51(1), 384. https://doi.org/10.1007/s11033-024-09277-z |
||||
| Kubba, M. A., Hussein, S. M., AL-Amier, F. H. G. Q., & Al-Zaidi, O. S. S. H. (2024). Genetic detection of prolactin intron 1 region in breast cancer patients of Iraqi women. International Journal of Science and Research Archive, 12(2), 2327-2340. https://doi.org/10.30574/ijsra.2024.12.2.1482 |
||||
| Kulibaba, R. O., Sakhatskyi, M. I., Griffin, D. K., & Romanov, M. N. (2024). Molecular diversity of Ukrainian native chicken breeds: a review. World's Poultry Science Journal, 80(4), 1265-1292. https://doi.org/10.1080/00439339.2024.2411401 |
||||
| Olaniyan, O. F., Oladejo, O. A., Adeola, A. C., Bello, S. F., Bashiru, H. A., & Oseni, S. O. (2024). Integration of genomics into community-based breeding programmes for chickens: an overview of opportunities, challenges, and potential benefits. World's Poultry Science Journal, 80(3), 977-997. https://doi.org/10.1080/00439339.2024.2354194 |
||||
| Oyebanjo, M. O., Osaiyuwu, O. H., & Salako, A. E. (2023). Prolactin, genetics, and bioinformatics: The trinity for improving duck egg production in Nigeria-A review. Journal of Animal Science and Veterinary Medicine, 8(5), 235-246. https://doi.org/10.31248/JASVM2023.398 |
||||
| Wang, C., Ren, Z., Liu, X., Song, X., Shi, L., Kang, H., Ma, D., Lan, X., Qu, L., Zhu, H., & Hua, J. (2025). The goat PRLR gene: mRNA expression, association analysis of InDel variants with body weight and growth traits. Gene, 935, 149081. https://doi.org/10.1016/j.gene.2024.149081 |
||||
| Zhu, T., & Li, W. (2024). The regulation of prolactin secretion and its targeting function of teleost. General and Comparative Endocrinology, 354, 114530. https://doi.org/10.1016/j.ygcen.2024.114530 |
||||
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