ISSN: 2536-7064
Model: Open Access/Peer Reviewed
DOI: 10.31248/JBBD
Start Year: 2016
Email: jbbd@integrityresjournals.org
https://doi.org/10.31248/JBBD2024.211 | Article Number: 378DF6E92 | Vol.9 (2) - June 2024
Received Date: 30 April 2024 | Accepted Date: 10 June 2024 | Published Date: 30 June 2024
Authors: Barrah Collins Chizaram* , Yakubu Ojochenemi Ejeh , Arowora Kayode Adebisi and Emmanuel Gabriel Onu
Keywords: Antioxidants, liver, Carbon Tetrachloride, Chromolaena odorata, medicinal.
Chromolaena odorata (C. odorata), has been used as a medicinal herb for various conditions, including malaria, inflammations, and more. This study aimed to assess the ameliorative effects and structure-function relationship of Chromolaena odorata leaf extract on Carbon Tetrachloride (CCl4)-induced hepatotoxicity in Wistar rats. The leaves of C. odorata were collected, washed and air-dried for one week at room temperature before ethanolic crude extraction and fractionation using column chromatography. Some of the fractions underwent GC-MS, FTIR and HPLC analyses. Forty-two male Wistar rats were randomly divided into 7 groups (n=6). The animals were administered the n-hexane fraction of the extract for twenty-one days before being euthanized for further examination. GC-MS analyses were conducted on selected fractions of the extract. Fractions were found to contain bioactive compounds like Nonyl octacosyl, Methyl stearate, etc. The FTIR analyses revealed peaks representing various functional groups. The HPLC findings indicated the presence of important compounds. Histopathological examination showed degrees of impairment in all groups except group 1, displaying a normal liver architecture. Results of the liver marker enzymes showed a significant increase (p<0.05) in ALT and AST levels across all test groups, except for group 3, where no significant change was observed compared to the normal control. Total protein exhibited a marked decrease in all test groups relative to the control, while albumin levels remained unchanged in all test groups compared to the control. Globulin levels significantly decreased (p<0.05) in groups 2, 4, 5, and 6, whereas groups 3 and 7 displayed no significant alteration compared to the normal control across all test groups. With exception of Catalase, which decreased non-significantly, Superoxide Dismutase and Catalase, decreased significantly in most of the test groups. The study suggests that the n-hexane fraction of ethanolic leaves extract of C. odorata may possess ameliorative effects on the liver, as evidenced by the reduction in liver function enzymes of the test groups when compared with the negative control.
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