JOURNAL OF BIOSCIENCE AND BIOTECHNOLOGY DISCOVERY
Integrity Research Journals

ISSN: 2536-7064
Model: Open Access/Peer Reviewed
DOI: 10.31248/JBBD
Start Year: 2016
Email: jbbd@integrityresjournals.org


Ameliorative effects and structure-function relationship of Chromolaena odorata (Linnaeus) King and Robinson leaf extract on carbon tetrachloride (CCl4)-induced hepatotoxicity in wistar rats

https://doi.org/10.31248/JBBD2024.211   |   Article Number: 378DF6E92   |   Vol.9 (2) - June 2024

Received Date: 30 April 2024   |   Accepted Date: 10 June 2024  |   Published Date: 30 June 2024

Authors:  Barrah Collins Chizaram* , Yakubu Ojochenemi Ejeh , Arowora Kayode Adebisi and Emmanuel Gabriel Onu

Keywords: Antioxidants, liver, Carbon Tetrachloride, Chromolaena odorata, medicinal.

Chromolaena odorata (C. odorata), has been used as a medicinal herb for various conditions, including malaria, inflammations, and more. This study aimed to assess the ameliorative effects and structure-function relationship of Chromolaena odorata leaf extract on Carbon Tetrachloride (CCl4)-induced hepatotoxicity in Wistar rats. The leaves of C. odorata were collected, washed and air-dried for one week at room temperature before ethanolic crude extraction and fractionation using column chromatography. Some of the fractions underwent GC-MS, FTIR and HPLC analyses. Forty-two male Wistar rats were randomly divided into 7 groups (n=6). The animals were administered the n-hexane fraction of the extract for twenty-one days before being euthanized for further examination. GC-MS analyses were conducted on selected fractions of the extract. Fractions were found to contain bioactive compounds like Nonyl octacosyl, Methyl stearate, etc. The FTIR analyses revealed peaks representing various functional groups. The HPLC findings indicated the presence of important compounds. Histopathological examination showed degrees of impairment in all groups except group 1, displaying a normal liver architecture. Results of the liver marker enzymes showed a significant increase (p<0.05) in ALT and AST levels across all test groups, except for group 3, where no significant change was observed compared to the normal control. Total protein exhibited a marked decrease in all test groups relative to the control, while albumin levels remained unchanged in all test groups compared to the control. Globulin levels significantly decreased (p<0.05) in groups 2, 4, 5, and 6, whereas groups 3 and 7 displayed no significant alteration compared to the normal control across all test groups. With exception of Catalase, which decreased non-significantly, Superoxide Dismutase and Catalase, decreased significantly in most of the test groups. The study suggests that the n-hexane fraction of ethanolic leaves extract of C. odorata may possess ameliorative effects on the liver, as evidenced by the reduction in liver function enzymes of the test groups when compared with the negative control.

Abei, H. (1974). Catalase, In: Method of enzymatic analysis. Academic Press, New York. Pp. 674-684.
https://doi.org/10.1016/B978-0-12-091302-2.50032-3
 
Akinmoladun, A. C., Ibukun, E. O., & Dan-Ologe, I. A. (2007). Phytochemical constituents and antioxidant properties of extracts from the leaves of Chromolaena odorata. Scientific Research and Essay, 2(6), 191-194.
 
Annisa, S., Erna, H., and Yudhi, N. (2021, November). The effectivities of anti-diabetic of Chromolaena odorata L. in lowering blood sugar level: A systematic review. In IOP Conference Series: Earth and Environmental Science (Vol. 913, No. 1, p. 012092). IOP Publishing.
https://doi.org/10.1088/1755-1315/913/1/012092
 
Anyasor, G. N., Aina, D. A., Olushola, M., & Aniyikaye, A. F. (2011). Phytochemical constituent, proximate analysis, antioxidant, antibacterial and wound healing properties of leaf extracts of Chromolaena odorata. Annals of Biological Research, 2(2), 441-451.
 
Bridget, O. I. and Usunomena, U. (2022). Aqueous Leaf Extract of Chromolaena odorata Attenuates Methotrexate-Induced Hepatoxicity in Wistar Rats. Journal of Fundamental and Applied Pharmaceutical Science, 3(1), 16-25.
https://doi.org/10.18196/jfaps.v3i1.15652
 
De Leon, J. A. D., & Borges, C. R. (2020). Evaluation of oxidative stress in biological samples using the thiobarbituric acid reactive substances assay. Journal of Visualized Experiments, 12(159), e61122.
 
Devakumar, J., Keerthana, V., & Sudha, S. (2017). Identification of Bioactive compounds by gas chromatography-mass spectrometry analysis of Syzygium Jambos (L.) collected from Western Ghats Region Coimbatore, Tamil Nadu. Asian Journal of Pharmaceutical and Clinical Research, 10(1), 364-369.
https://doi.org/10.22159/ajpcr.2017.v10i1.15508
 
El-Beshbishy, H. A., Mohamadin, A., Nagy, A., & Abdel-Naim, A. (2010). Amelioration of tamoxifen-induced liver injury in rats by grape seed extract, black seed extract and curcumin. Indian Journal of Experimental Biology, 48, 280-288.
 
El-Demerdash, F. M., Yousef, M. I., Kedwany, F. S., & Baghdadi, H. H. (2004). Cadmium induced changes in lipid peroxidation, blood hematology, biochemical parameters and semen quality of male rats: Protective role of vitamin E and β-carotene. Food and Chemical Toxicology, 42(10),1563-1571.
https://doi.org/10.1016/j.fct.2004.05.001
 
El-Mahmood, M. A. (2009). Antibacterial activity of crude extracts of Euphorbia hirta against some bacteria associated with enteric infections. Journal of Medicinal Plants Research, 3(7), 498-505.
 
Giannini, E. G., Test, R., & Savarino, V. (2005). Liver enzyme alteration: a guide for clinicians. Canadian Medical Association Journal, 172(3), 367-379.
https://doi.org/10.1503/cmaj.1040752
 
Harborne, J. B., & Williams, C. A. (2000). Advances in flavonoid research since 1992. Phytochemistry, 55, 481-504.
https://doi.org/10.1016/S0031-9422(00)00235-1
 
Hoareau, L., & DaSilva, E. J. (1999). Medicinal plants: a re-emerging health aid. Electronic Journal of biotechnology, 2(2), 3-4.
https://doi.org/10.2225/vol2-issue2-fulltext-2
 
Imo, C., Uhegbu, F. O., & Ifeanacho, N. G. (2015). Effect of exposure to inhalation of selected petroleum products on liver function of male albino rats: A comparative study. IOSR Journal of Environmental Science, Toxicology and Food Technology, 19, 2319-99.
 
Kamisan, F., Yahya, F., Mamat, S., Kamarolzaman, M., Mohtarrudin, N., Kek, T., Salleh, M., Hussain, M., & Zakaria, Z. (2014). Effect of methanol extract of Dicranopteris linearis against carbon tetrachloride-induced acute liver injury in rats. BMC Complementary and Alternative Medicine, 14, 123-132.
https://doi.org/10.1186/1472-6882-14-123
 
Krier, M., & Ahmed, A. (2009). The asymptomatic outpatient with abnormal liver function tests. Clinical Liver Disease, 13(2),167-177.
https://doi.org/10.1016/j.cld.2009.02.001
 
Lachman, J., Hamouz, K., Orsak, M., & Pivec, V. (2000). Potato tubers as a significant source of antioxidants in human nutrition. Rostlinna Vyroba, 46(5), 231-236.
 
McCord, J., & Fridovich, I. (1969). Superoxide dismutase, enzymic function for erythrocuprein. Journal of Biological Chemistry, 6, 126-130.
 
McFadyen, R. E. C. (2004). Chromolaena in East timor: history, extent and control. In: Day, M. D., & McFadyen, R. E. (eds.). Proceedings of the sixty international Workshop on Biological Control and Management of Chromolaena odorata (pp 8-10) ACIAR Technical Report 55. Canbra, Australia.
 
Nascimento, G. G. F., Locatelli, J., Freitas, P. C., & Silva, G. L. (2000). Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiology, 31(4), 247-256.
https://doi.org/10.1590/S1517-83822000000400003
 
Nutmakul, T. (2022). A review on benefits of quercetin in hyperuricemia and gouty arthritis. Saudi Pharmaceutical Journal, 30(7), 918-926.
https://doi.org/10.1016/j.jsps.2022.04.013
 
Obadoni, B. O., & Ochuko, P. O. (2002). Phytochemical studies and comparative efficacy of the crude extracts of some haemostatic plants in Edo and Delta States of Nigeria. Global Journal of Pure and Applied Sciences, 8(2), 203-208.
https://doi.org/10.4314/gjpas.v8i2.16033
 
Okigbo, R. N, Eme, U. E., & Ogbogu, S. (2008). Biodiversity and conservation of medicinal and aromatic plants in Africa. Biotechnology and Molecular Biology Reviews, 3(6), 127-134.
 
Ordonez, A. A. I., Gomez, J. D., Vattuone, M. A., & Lsla, M. I. (2006). Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food Chemistry, 97, 452-458.
https://doi.org/10.1016/j.foodchem.2005.05.024
 
Owolabi, O. J., Omogbai, E. K. I., and Obasuyi, O. (2007). Antifungal and antibacterial activities of the ethanolic and aqueous extract of Kigelia africana (Bignoniaceae) stem bark. African Journal of Biotechnology, 6(14), 1677-1680.
 
Ozgen, S., Kilinc, O. K., Selamoglu, Z. (2016). Antioxidant activity of quercetin: A mechanistic review Kuersetin Antioksidan Aktivitesi: Mekanik Bir Derleme. Turkish Journal of Agriculture - Food Science and Technology, 4, 1134-1138.
https://doi.org/10.24925/turjaf.v4i12.1134-1138.1069
 
Phan, T. T., Wang, L., See P, Grayer, R. J., Chan, S. Y., & Lee, S. T. (2001). Phenolic compounds of Chromolaena odorata protect cultured skin cells from oxidative damage: Implication for cutaneous wound healing. Biological and Pharmaceutical Bulletin, 24(12), 1373-1379.
https://doi.org/10.1248/bpb.24.1373
 
Rauf, A., Imran, M., Khan, I. A., ur‐Rehman, M., Gilani, S. A., Mehmood, Z., & Mubarak, M. S. (2018). Anticancer potential of quercetin: A comprehensive review. Phytotherapy Research, 32(11), 2109-2130.
https://doi.org/10.1002/ptr.6155
 
Revathi, P., Jeyaseelansenthinath, T., & Thirumalaikolundhusubramaian, P. (2014). Preliminary phytochemical screening and GC-MS analysis of ethanolic extract of mangrove plant- Bruguiera cylindrica (rhizho) L. International Journal of Pharmacognosy and Phytochemical Research, 6(4), 729-740.
 
Rowdhwal, S. S. S., & Chen, J. (2018). Toxic effects of di‐2‐ethylhexyl phthalate: An overview. BioMed research international, Volume 2018, Article ID 1750368, 10 pages.
https://doi.org/10.1155/2018/1750368
 
Singleton, V. L., Orthofer, R., & Lamuela-Raventos, R. M. (2002). Analysis of total phenol and other oxidative substrates and antioxidants by means of Folin-ciocalteu reagent. Methods in Enzymology, 299:152-178.
https://doi.org/10.1016/S0076-6879(99)99017-1
 
Taiwo, O. B., Olajide, O. A., Soyannwo, O. O., & Makinde, J. M. (2000). Anti-inflammatory, Antipyretic and Antispasmodic Properties of Chromolaena odorata. Pharmaceutical Biology, 38(5), 367-370.
https://doi.org/10.1076/phbi.38.5.367.5970
 
Ugwu, O. P. C., Obeagu, E. I., Alum, E. U., Okon, M. C., Aja P. M., Amusa, M. O., Adepoju, A. O., & Samson, A. O. (2023). Effect of Ethanol Leaf extract of Chromolaena odorata on hepatic markers in streptozotocin-induced diabetic wistar albino rats. IAA Journal of Applied Sciences, 9(1), 46-56.
 
Umaru, I. J., Badruddin, F. A., & Umaru, H. A. (2018). Phytochemical, antifungal and antibacterial potential of Leptadenia hastata stem-bark extract. MOJ Toxicology, 4(4), 263-268.
https://doi.org/10.15406/mojt.2018.04.00110
 
Urquiaga, I. N. E. S., & Leighton, F. (2000). Plant polyphenol antioxidants and oxidative stress. Biological research, 33(2), 55-64.
https://doi.org/10.4067/S0716-97602000000200004
 
Vaisakh, M. N., & Pandey, A. (2012). The invasive weed with healing properties: A review on Chromolaena odorata. International Journal of Pharmaceutical Sciences and Research, 3(1), 80-83.
 
Yakubu, O. E., Isaac, U. J., Emochone, Y. R., & Ehi, O. T. (2022). Purification and GCMS spectroscopic identification of active antioxidant principles in ethanol extract of Phyllanthus amarus leaves. American Journal of Biochemistry and Molecular Biology, 12 (1), 30-40.
https://doi.org/10.3923/ajbmb.2022.30.40
 
Yakubu, O. E., Nwodo, O. F. C., Joshua, P. E., Ugwu, M. N., Odu, A. D., & Okwo, F. (2014). Fractionation and determination of total antioxidant capacity, total phenolics and total flavonoids contents of aqueous, ethanol and nHexane extracts of Vitex doniana leaves. African Journal of Biotechnology, 13(5), 693-8.
https://doi.org/10.5897/AJB2013.13225
 
Zakharova, O. D, Frolova, T. S, Yushkova, Y. V., Chernyak, E. I., Pokrovsky, A. G. and Pokrovsky, M. A. (2016). Antioxidant and antitumor activity of trolox, trolox succinate, and α-tocopheryl succinate conjugates with nitroxides. European Journal of Medicinal Chemistry, 122, 127-137.
https://doi.org/10.1016/j.ejmech.2016.05.051