JOURNAL OF BIOSCIENCE AND BIOTECHNOLOGY DISCOVERY
Integrity Research Journals
ISSN: 2536-7064
Model: Open Access/Peer Reviewed
DOI: 10.31248/JBBD
Start Year: 2016
Email: jbbd@integrityresjournals.org
Evaluation of wound healing potential of Jatropha curcas leaf extracts ointment based on wound infection in Wistar rats model
https://doi.org/10.31248/JBBD2022.167 | Article Number: 18C758822 | Vol.7 (3) - October 2022
Received Date: 04 September 2022 | Accepted Date: 10 October 2022 | Published Date: 30 October 2022
Authors: Adamu S. Mohammed* , Abalaka M. Enemaduku , Safiya D. Yahaya , Abdullahi Muhammad and Kolo O. Oyeronke
Keywords: herbal ointment, J. curcas leaf extracts, Staphylococcus aureus, wound healing.
This research study was conducted to evaluate the wound healing potential of ointment formulated with Jatropha curcas leaf extracts based on wistrar rat model. The therapeutic activities of the plant extracts were determined by topical application of the herbal ointment. Wound healing rate took place on the 16th day of post treatment with 1.0 g of J. curcas ointment formulation and 12th day with 2.0 g of the ointment, was similar compared with standard drug Gentamicin ointment (12 days). The control untreated group (62.3±0.51%) rate of wound closure observed persisted beyond the 19th day of post-wounding. The haematological parameters of the infected rats treated with herbal ointment and the control group untreated were not significant different (p>0.05). Significant increase in white blood cell (WBC) count in untreated group was recorded when compared with the treated group. However, treatment with the formulated ointments significantly increase the elevated WBC count in the treated group. The toxicological parameters in the serum of the rats are useful makers for the assessment of tissue damage. The serum Aspartate Transaminase (AST) and Alanine Transaminase (ALT) activities in the treated and untreated experimental groups were not significantly (p>0.05) different. The phytochemical components found in J. curcas leaves contain some useful potential antimicrobial agents that possess wound healing properties when formulated into a topical ointment for topical application.
| Aderogba, M. A., Okoh, E. K., Okeke, I. N., Olajide, A.O., & Ogundaini, A. O. (2006). Antimicrobial and anti-inflammatory effects of Piliostigma reticulatum leaf extract. International Journal of Pharmacology, 2(1), 70-74. Crossref |
||||
| Aiyegoro, O. A., & Okoh, A. I. (2009). Use of bioactive plant products in combination with standard antibiotics: Implications in antimicrobial chemotherapy. Journal of Medicinal Plants Research 3(13), 1147-1152. | ||||
| Alzohairy, M. A. (2011). Colonization and antibiotic susceptibility pattern of methicillin resistance Staphylococcus aureus (MRSA) among farm animals in Saudi Arabia. Journal of Bacteriology Research. 3(4),63-68. | ||||
| Atangwho, I. J. (2009). Comparative chemical composition of leaves of some antidiabetic medicinal plants: Azadirachta indica, Vernonia amygdalina and Gongronema latifolium. African Journal Biotechnology, 8(9), 685-689. | ||||
| Bannerman, T. L. (2003). Staphylococcus, Micrococcus, and other catalase-positive cocci that grow aerobically. In: Murray, P. R., Baron, E. J., Jorgensen, J. H., Pfaller, M. A., & Yolken, R. H. (eds.). Manual of clinical microbiology, 8th ed. American Society for Microbiology, Washington, D.C. | ||||
| Bashir, L., Shittu, O. K., Busari, M. B, Sani, S., & Aisha M. I. (2015). Safety evaluation of giant African land snails (Archachatina marginata) Haemolymph on hematological and biochemical parameters of albino rats. Journal of Advances in medical and Pharmaceutical Sciences, 3(3), 122-130. Crossref |
||||
| Canadian Council on animal care (CCAC) guideline on animal use protocol review (1997). | ||||
| Cheesbrough, M (2002). Biochemical tests to identify bacteria. District laboratory practice in tropical countries, Part II. Cambridge University Press, Britain Pp. 67-68. | ||||
| Chika, O., Ifeanyi, N., Okoli, C., & Beatrice, N. A. (2007). Antibacterial activities and toxicological potential of crude ethanolic extracts of Euphorbia hirta. Journal of American science, 3(3), 11-16 | ||||
| Clinical and Laboratory Standard Institute (CLSI) (2009). Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). | ||||
| Daniyan, S. Y. & Abalaka, M. E. (2012). Antimicrobial activity of leaf extracts of Piliostigma thonningii. Journal of Science and Multidisciplinary Research, 1(2), 8-13. | ||||
| Hallin, M., Denis, O., Deplano, A., De Ryck, R., Crèvecoeur, S., Rottiers, S., De Mendonça, R., & Struelens, M. J. (2008). Evolutionary relationships between sporadic and epidemic strains of healthcare-associated methicillin-resistant Staphylococcus aureus. Clinical Microbiology and Infection, 14(7), 659-669. Crossref |
||||
| Harbarth, S., François, P., Schrenzel, J., Fankhauser-Rodriguez, C., Hugonnet, S., Koessler, T., Huyghe, A., & Pittet, D. (2005). Community-associated methicillin-resistant Staphylococcus aureus, Switzerland. Emerging Infectious Diseases, 11(6), 962-965. Crossref |
||||
| Harbone, J. B. (1994). Phytochemical methods. Chapman and Hall Limited London. Pp. 49-188. | ||||
| Heller J. (2013). Physic nut, Jatropha curcas L. Promoting the conservation and use of underutilized and neglected crops. Institute of Plant Genetics and Crop Plant Research. Gartersleben, International Plant Genetic Resources Institute, Rome. | ||||
| Hugo, W. B., &. Russell, A.D., (2003). Pharmaceutical Microbiology. 6th (edn). Blackwell Science Publishers. p. 117. | ||||
| Ikobi, E., Igwilo, C. I., Awodele, O., & Azubuike, C. (2012). Antibacterial and wound healing properties of methanolic extracts of dried fresh Gossypium barbadense Leaves. Asian Journal of Biomedical and Pharmaceutical Sciences 2(13), 32-37. | ||||
| Kodati, D. R., Burra, S., & Goud, P. K. (2011). Evaluation of wound healing activity of methanolic root extract of Plumbago zeylanica L. in wistar albino rats. Asian Journal of Plant Science and Research, 1(2), 26-34. | ||||
| Lawal, B., Shittu, O. K., Abubakar, A. N., Haruna, G. M., Sani, S., & Ossai, P. C. (2015b). Haematopoetic effect of methanol extract of Nigerian honey bee (Apis mellifera) propolis in mice. Journal of Coastal Life Medicine.3(8), 648-651. Crossref |
||||
| Lawal, B., Shittu, O. K., Prince, C. O., Asmau, A. N., & Aisha, M. I. (2015a). Evaluation of antioxidant activity of giant Africa snail (Archachatina marginata) Haemolymph in CCl4- induced hepatotoxixity in albino Rats. British Journal Pharm. Research, 6(3), 141-154. Crossref |
||||
| Nascimento, G. G., Locatelli, J., Freitas, P. C., & Silva, G. L. (2000). Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiology, 31(4), 247-256. Crossref |
||||
| Nath, L. K., & Dutta, S. K. (2012). Acute toxicity studies and wound healing response of curcain, a proteolytic enzyme extract from the latex of Jatropha curcas L. In: Gubitz, G. M., Mittelbach, M., & Trabi, M. (eds.). Biofuels and Industrial Products from Jatropha curcas. DBV Graz. Pp. 82-86. | ||||
| Okoli, C. O., Akah, P. A., Ezike, A. C., Udegbunam, S., Nworu, S. C., & Okoye, T. C. (2008). Ethnobiology and pharmacology of Jatropha curcas L. Research Signpost: India, Pp. 101-125. | ||||
| Parish, M. E, & Davidson, P. M. (1993). Methods for evaluation: In antimicrobials in foods, 2nd Edition. Marcel Dekker, Inc., New York. Pp. 597-615. | ||||
| Salau, A. K., Akanji M. A., & Yakubu, M. T. (2013). Evaluation of aqueous extract of Crateva adansonii leaves on selected tissues of rats. Fountain Journal of Natural and Applied Science, 2(1), 17-28. Crossref |
||||
| Shittu, O. K., Lawal. B., Haruna, G. M, Berinyuy, E B., Yusuf, A. A., & Ibrahim, A. M. (2015). Hepato-curative effects of methanol extract from Nigeria bee propolis in wound healing activity, 12(4), 45-51. | ||||
| Trease, G. E., & Evans, W. C. (2004). Phytochemical screening of powdered roots, stem, leaves Pharmacology 13th Edition Tindali. Pp. 1124-1125. | ||||
| Udupa, S. L, Shetty, S, Udupa, A. L., & Somayaji, S. N. (2006). Effect of Ocimum sanctum Linn. on normal and dexamethason suppressed wound healing. Indian Journal Experimental Biology, 44, 49-54. | ||||
| Yakubu, M. T, & Musa, I. F. (2012). Liver and kidney functional indices of pregnant rats following the administration of the crude alkaloids from Senna alata (Linn.Roxb) leaves. Iranian Journal of Toxicology, 6(16), 615-625. | ||||
| Yakubu, M. T., Bilbis, L. S., Lawal, M., & Akanji, M. A. (2003). Effect of repeated administration of sildenafil citrate on selected enzyme activities of liver and kidney of male albino rats. Nigerian Journal of Pure Applied Science, 18, 1395-1400. | ||||
Copyright © 2025 Integrity Research Journals. All rights reserved.