JOURNAL OF BIOSCIENCE AND BIOTECHNOLOGY DISCOVERY
Integrity Research Journals

ISSN: 2536-7064
Model: Open Access/Peer Reviewed
DOI: 10.31248/JBBD
Start Year: 2016
Email: jbbd@integrityresjournals.org


Genotoxicity, behaviour and erythrocytic- oxidative stress modulation of clariid catfish to Acetamenophen N-(4-hydroxyphenyl) ethanamide EC number: 203-157-5 | CAS number: 103-90-2

https://doi.org/10.31248/JBBD2019.114   |   Article Number: 0ED30CD26   |   Vol.4 (4) - August 2019

Received Date: 25 July 2019   |   Accepted Date: 28 August 2019  |   Published Date: 30 August 2019

Authors:  Christopher Onyemaechi Ezike* , Felix Okaliwe Echor , Nicholas Chinwe Uwadiegwu and Callistus Irechukwu Nwosu

Keywords: toxicity, oxidative stress, Antioxidants, genotoxicity, paracetamol, peroxidation

This study was undertaken to evaluate the acute toxicity and sub-acute paracetamol toxicity-induced nuclear aberration, antioxidative stress enzyme activities such as CAT, SOD, LPO, GPX of Clarias gariepinus. Three hundred (300) juveniles with average weight of 50.96±1.3g were procured from a reputable fish farm in Enugu to the Fisheries Research Laboratory (latitude 6.4659oN and longitude 7.57620E). 180 juveniles were exposed to acute concentrations of 5000, 5500, 6000, 6500, 7000mgL-1 and control in triplicate replication while remaining 120 fish in similar replication were exposed to 620, 1240, 3100 mgL-1corresponding to 1/10, 1/5 and ½ 96hour LC50 = 6200 mgL-1, y = 14.23x - 48.96, R² = 0.963, safety dose 1/100 LC50 = 62 mgL-1. Blood samples were collected from the caudal vein on day 2, 4, and 8 for erythrocyte-oxidative enzyme activities. Data was subjected to one way analysis of variance to determine the significant differences between treatments and control at p<0.05 using computer software SPSS version 20 and was expressed as means plus standard error of means. The exposed fish showed behavioral changes (erratic movement, air gulping, nervous manifestation and imbalance) before death. CAT reduced significantly below the control value of 0.83 to the least value of 0.57 µmol mm-1 mg protein-1 among the highest group exposed to 3100 mg/L. Similarly, SOD activity in exposed group of fish varied significantly from the control and ranged from the highest control value of 12.31 to the least value of 8.27 U mol mm-1 mg protein-1among fish to the highest concentration of the drug. GPX ranged from 9 to 4.65 µmol mm-1 mg protein-1 among group of fish in the control to 3100mg/L of the drug. On the other hand, LPO increased significantly (p<0.05) from the control value of 6.83 to 8 mMole/TBARS/mg protein among group of fish to 3100 mg/L of the drug indicative of a dose dependent elevation of micronuclei The use of genotoxic potential and oxidative stress modulation by fish in inland water bodies can be a useful diagnostic tool to measure the health alterations in C. gariepinus which may serve as early warning indicators of pollution.

Alla, M. M. N., Badawi, A. H. M., Hassan, N. M., El-Bastawisy, Z. M., & Badran, E. G. (2007). Induction of glutathione and glutathione-associated enzymes in butachlor-tolerant plant species. American Journal Plant Physiology, 2(3), 195-205.
Crossref
 
Al-Sabti, K., & Metcalfe, C. D. (1995). Fish micronuclei for assessing genotoxicity in water. Mutation Research/Genetic Toxicology, 343(2-3), 121-135.
Crossref
 
American Public Health Association, American works Association and water Environmental Federation (APHA) (2005). Standard Methods for the Examination of Water and Wastewater 21st, ed. APHA, Washington, DC. Pp. 20001-23710.
 
Ansari, R. A., Rahman, S., Kaur, M., Anjum, S., & Raisuddin, S. (2011). In vivo cytogenetic and oxidative stress-inducing effects of cypermethrin in freshwater fish, Channa punctate Bloch. Ecotoxicology and Environmental Safety, 74(1), 150-156.
Crossref
 
Azad, I. S., Panigrahi, A., Gopal, C., Paulpandi, S., Mahima, C., & Ravichandran, P. (2005). Routes of immunostimulation vis-a-vis survival and growth of Penaeus monodon postlarvae. Aquaculture, 248(1-4), 227-234.
Crossref
 
Bagnyukova, T. V., Chahrak, O. I., & Lushchak, V. I. (2006). Coordinated response of goldfish antioxidant defenses to environmental stress. Aquatic toxicology, 78(4), 325-331.
Crossref
 
Chanu, T. I., Roy, S. D. A. M., Sharma, A., Biswas, P., Siddhya, G. M., & Das, A. (2014). Anti-stress potential of acetone extract of Zingiber officinale Roscoe on biochemical and oxidative stress parameters in Labeo calbasu (Hamilton 1822) fingerlings subjected to acid stress. Indian Journal Fisheries, 61(4), 69-77.
 
Chelikani, P., Fita, I., & Loewen, P. C. (2004). Diversity of structures and properties among catalases. Cellular and Molecular Life Sciences, 61(2), 192-208.
Crossref
 
Finney, D. T. (1971). Probit analysis. Cambridge University press. Cambridge. p. 333.
 
Ghelfi, A., Ribas, J. L. C., Guiloski, I. C., Bettim, F. L., Piancini, L. D. S., Cestari, M. M., Pereira, A.J., Sassaki, G. L., & de Assis, H. C. S. (2016). Evaluation of biochemical, genetic and hematological biomarkers in a commercial catfish Rhamdia quelen exposed to diclofenac. Bulletin of Environmental Contamination and Toxicology, 96(1), 49-54.
Crossref
 
Kim, Y., Choi, K., Jung, J., Park, S., Kim, P. G., & Park, J. (2007). Aquatic toxicity of acetaminophen, carbamazepine, cimetidine, diltiazem and six major sulfonamides, and their potential ecological risks in Korea. Environment International, 33(3), 370-375.
Crossref
 
Maran, E., Fernández, M., Barbieri, P., Font, G., & Ruiz, M. J. (2009). Effects of four carbamate compounds on antioxidant parameters. Ecotoxicology and Environmental Safety, 72(3), 922-930.
Crossref
 
Misra, H. P., & Fridovich, I. (1972). The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological Chemistry, 247(10), 3170-3175.
 
Monteiro, D. A., De Almeida, J. A., Rantin, F. T., & Kalinin, A. L. (2006). Oxidative stress biomarkers in the freshwater characid fish, Brycon cephalus, exposed to organophosphorus insecticide Folisuper 600 (methyl parathion). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 143(2), 141-149.
Crossref
 
Nunes, B., Antunes, S. C., Santos, J., Martins, L., & Castro, B. B. (2014). Toxic potential of paracetamol to freshwater organisms: a headache to environmental regulators? Ecotoxicology and Environmental Safety, 107, 178-185.
Crossref
 
Organization for Economic Co-operation and Development (OECD) (1992). Guideline 203. OECD Guideline for Testing of Chemicals: Fish, Acute Toxicity Test.
 
Orhan, H., & ┼×ahin, G. (2001). In vitro effects of NSAIDS and paracetamolon oxidative stress-related parameters of human erythrocytes. Experimental and Toxicologic Pathology, 53(2-3), 133-140.
Crossref
 
Paglia, D. E., & Valentine, W. N. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of Laboratory and Clinical Medicine, 70(1), 158-169.
 
Pandey, S., Ahmad, I., Parvez, S., Bin-Hafeez, B., Haque, R., Raisuddin, S. (2001). Effect of endosulfan on antoxidants in freshwater fish Channa punctatus Bloch. Protection against lipid peroxidation in liver by copper pre-exposure. Archives of Environmental Contamination and Toxicology, 41(3), 345-352.
Crossref
 
Pandey, S., Parvez, S., Sayeed, I., Haque, R., Bin-Hafeez, B., & Raisuddin, S. (2003). Biomarkers of oxidative stress: a comparative study of river Yamuna fish Wallago attu (Bl. & Schn.). Science of the Total Environment, 309(1-3), 105-115.
Crossref
 
Parolini, M., Binelli, A., Cogni, D., & Provini, A. (2010). Multi-biomarker approach for the evaluation of the cyto-genotoxicity of paracetamol on the zebra mussel (Dreissena polymorpha). Chemosphere, 79(5), 489-498.
Crossref
 
Roberts, P. H., & Thomas, K. V. (2006). The occurrence of selected pharmaceuticals in wastewater effluent and surface waters of the lower Tyne catchment. Science of the Total Environment, 356(1-3), 143-153.
Crossref
 
Sanderson, H., Johnson, D. J., Reitsma, T., Brain, R. A., Wilson, C. J., & Solomon, K. R. (2004). Ranking and prioritization of environmental risks of pharmaceuticals in surface waters. Regulatory Toxicology and Pharmacology, 39(2), 158-183.
Crossref
 
Scandalios, J. G. (2005). Oxidative stress: Molecular perception and transduction of signals triggering antioxidant gene defenses. Brazilian Journal of Medical and Biological Research, 38(7), 995-1014.
Crossref
 
Sharma, S. K., & Murti, C. K. (1968). Production of lipid peroxides by brain. Journal of Neurochemistry, 15(2), 147-149.
Crossref
 
Singh, S., Singh, S. K., Kumar, M., Chandra, K., & Singh, R. (2011). Ameliorative potential of quercetin against paracetamol-induced oxidative stress in mice blood. Toxicology International, 18(2), 140-145.
Crossref
 
Sogbanmu, T. O., Osibona, A. O., Oguntunde, O. A., & Otitoloju, A. A. (2018). Biomarkers of toxicity in Clarias gariepinus exposed to sublethal concentrations of polycyclic aromatic hydrocarbons. African Journal of Aquatic Science, 43(3), 281-292.
Crossref
 
Storey, K. B. (1996). Oxidative stress: animal adaptations in nature. Brazilian Journal of Medical and Biological Research, 29, 1715-1733.
 
Takahara, S., Humilton, H. B., Neel, J. V., Kobara, T. Y., Ogura, Y., Nishimura, E. T. (1960). Hypocalalasemia, a new genetic career state. Journal of Clinical Investigation, 39, 610-619.
Crossref
 
Thomas, K. V., Dye, C., Schlabach, M., & Langford, K. H. (2007). Source to sink tracking of selected human pharmaceuticals from two Oslo city hospitals and a wastewater treatment works. Journal of Environmental Monitoring, 9(12), 1410-1418.
Crossref
 
Velma, V., & Tchounwou, P. B. (2010). Chromium-induced biochemical, genotoxic and histopathologic effects in liver and kidney of goldfish, Carassius auratus. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 698(1-2), 43-51.
Crossref
 
Yousef, M. I., Omar, S. A., El-Guendi, M. I., & Abdelmegid, L. A. (2010). Potential protective effects of quercetin and curcumin on paracetamol-induced histological changes, oxidative stress, impaired liver and kidney functions and haematotoxicity in rat. Food and Chemical Toxicology, 48(11), 3246-3261.
Crossref
 
Zhang, J., Shen, H., Wang, X., Wu, J., & Xue, Y. (2004). Effects of chronic exposure of 2, 4-dichlorophenol on the antioxidant system in liver of freshwater fish Carassius auratus. Chemosphere, 55(2), 167-174.
Crossref
 
Zheng, Z. L., Tan, J. Y., Liu, H. Y., Zhou, X. H., Xiang, X., & Wang, K. Y. (2009). Evaluation of oregano essential oil (Origanum heracleoticum L.) on growth, antioxidant effect and resistance against Aeromonas hydrophila in channel catfish (Ictalurus punctatus). Aquaculture, 292(3-4), 214-218.
Crossref