Integrity Research Journals

ISSN: 2536-7064
Model: Open Access/Peer Reviewed
DOI: 10.31248/JBBD
Start Year: 2016

Isolation and characterization of bioactive phytochemicals from chloroform extract of Adenodolichos paniculatus (hua) Hutch. & Dalz (Fabaceae)   |   Article Number: 05C8B4F21   |   Vol.6 (3) - August 2021

Received Date: 09 August 2021   |   Accepted Date: 28 August 2021  |   Published Date: 30 August 2021

Authors:  Kyahar, I. F.* , Onwuliri, A. E. , Ehinmidu, J. O. and Oladosu, P. O.

Keywords: phytochemicals., characterization, Isolation, Adenodolichos paniculatus, roots.

Phytochemicals are the chemicals extracted from plants which make them significant source of drugs with potential for thousands of years. Adenodolichos paniculatus have been observed to have some medicinal applications. The aim of this work was to isolate and characterize some bioactive phytochemicals from the chloroform root extract of A. paniculatus which may justify its use by traditional healers. A. paniculatus roots were collected, identified, dried and pulverized.  The pulverized plant material was subjected to serial exhaustive extraction using n-hexane, chloroform, ethyl acetate, methanol and water. Vacuum liquid chromatography of the chloroform root extract led to a number of fractions. TLC analysis was used to study the different fractions. This isolation and purification afforded a dark orange liquid which was subjected to physical spectroscopic identification using IR and GC-MS. The major phytochemicals identified were 9,12-octadecadienoic acid (linoleic acid), undecanoic acid, 10-bromo-(10-bromoudecanoic acid), octadecanoic acid (stearic acid) and n-hexadecanoic acid (palmitic acid). These findings support the traditional use of Adenodolichos paniculatus in various diseases treatments specifically sore throat infections.

Aadesariya K.M, Ram R.V and Dave N.P (2017). Soxhtherm extraction, isolation and identification of fatty acids present in the hexane extract of Abutilon pannosum and Grewia using Gas chromatography-Mass Spectrometry. International Journal of Advanced Research in Chemical Sciences, 4(10), 26-34.
Agilent (2013a). Agilent 7890B Gas Chromatography operation manual, First edition, Agilent Technologies Inc. Wilmington, DE 19808-1610 USA.
Agilent (2013b). Agilent Cary 630 FTIR operation manual, First ed., Agilent Technologies Inc. Wilmington, DE 19808-1610 USA.
Aliyu, M., Kano, M. A., Abdullahi, N., Kankara, I. A., Ibrahim, S. I., & Muhammad, Y. Y. (2017). Extraction, characterization and fatty acids profiles of Nymphaea Lotus and Nymphaea pubescens seed oils. Biosciences Biotechnology Research Asia, 14(4), 1299-1307.
Banu, K. S., & Catherine, L. (2015). General techniques involved in phytochemical analysis. International Journal of Advanced Research in Chemical Sciences, 2(4), 25-32.
Bulama J. S, Dangoggo S. M, Halilu M. E, Tsafe A. I., & Hassan, S. W (2014). Isolation and characterization of palmitic acid from ethyl acetate extract of root bark of Terminalia glaucescens. Chemistry and Materials Research, 6(12), 140-144.
Burkill, H. M. (1985a). The useful plants of Tropical West Africa: Families A D. Kew. Royal Botanical Garden, Kew. Pp. 1-254.
Burkill, H. M. (1985b). The useful plants of West Tropical Africa: Families A D. Kew. Royal Botanic Gardens, Pp. 1-319.
Dilika, F., Bremner P. D., & Meyer, J. J. (2000). Antibacterial activity of linoleic and oleic acids isolated from Helichrysum pedunculatun: A plant used during circumcision rites. Fitoterapia, 71(14), 450-452.
Doan, L. P., Nguyen, T. T, Pham, D. Q., Than, V. T., & Bach, L. G. (2019). Extraction process, identification of fatty acids, tocopherols, sterols and phenolic constituents and antioxidant evaluation of seed oils from five Fabaceae species. Process, 7(7), 456.
Hui, L. Y., Shunsheng, C., Xiaolin, X., Manman, Z., Wenfang, Z., Kewu, L., & Kehai, L. (2015). Isolation of linoleic acid from Sambucus williamsii seed oil extracted by high pressure fluid and its antioxidant, antiglycemic, hypolipidemic activities. International Journal of Food Engineering, 11(3), 383-391.
Hutchinson, J., & Dalziel, J. M. (1958). Caesalpiniaceae, Mimosaceae and Papilionaceae. Flora of West Tropical Africa, 1(2), 439-587.
Isyaku, I. (2018). Isolation, characterization and antimicrobial activity of bioactive constituents from the leaf extract of Adenodolichos paniculatus.
Isyaku, I., Bello, A., Ndukwe, I., & Kizito, G. (2020). Isolation and characterization of nonanoic acid from ethyl acetate extract of Adenodolichos paniculatus. Communication in Physical Sciences, 5(3), 337-342.
Karimi, E., Jaafar, H. Z., Ghasemzadeh, A., & Ebrahimi, M. (2015). Fatty acid composition, antioxidant and antibacterial properties of the microwave aqueous extract of three varieties of Labisia pumila Benth. Biological Research, 48, Article Number 9.
Koroma, L., Yormah, T. B. R., Kamara, L. M., & Robert, G. M. T. (2018). Extraction and Characterization of Linoleic Acid from the Leaves of the Traditional Medicinal Plant Caloncoba echinata in Sierra Leone. American Scientific Research Journal for Engineering, Technology, and Sciences, 45(1), 185-206.
Mensah-Agyei, G. O., Ayeni, K. I., & Ezeamagu, C. O. (2020). GC-MS analysis of bioactive compounds and evaluation of antimicrobial activity of the extracts of Daedalea elegans: A Nigerian mushroom. African Journal of Microbiology Research, 14(6), 204-210.
Okwuchi, N. P. (2015). Extraction, fractionation and assessment of antioxidant activities of active components of Aframomum sceptrum seeds. African Journal of Biochemistry Research, 9(10), 117-123.
Raju, P., Sateesh, P., Venkanna, L., & Estari, M. (2014). Preliminary phytochemical investigation and TLC analysis of Physalis angulata fruit extract. Journal of Pharmacy and Biological Sciences, 9(2), 11-14.
Sani, M., Anuka, J., Magaji, I., Yaro, A., Magaji, M., & Sani, Y. (2010). Evaluation of analgesic and anti-inflammatory activities of the methanolic leaf extract of Adenodolichos paniculatus (hua). Nigerian Journal of Pharmaceutical Sciences, 9(1), 73-80.