JOURNAL OF ANIMAL SCIENCE AND VETERINARY MEDICINE
Integrity Research Journals
ISSN: 2536-7099
Model: Open Access/Peer Reviewed
DOI: 10.31248/JASVM
Start Year: 2016
Email: jasvm@integrityresjournals.org
Kinematic responses of cold-preserved rote ram sperm to supplementation with bovine serum albumin and moringa leaf extract
https://doi.org/10.31248/JASVM2026.644 | Article Number: C731A2E26 | Vol.11 (2) - April 2026
Received Date: 02 March 2026 | Accepted Date: 23 March 2026 | Published Date: 30 April 2026
Authors: T. M. Hine* , W. M. Nalley , K. Uly , P. Kune , A. R. Riwu , A. B. Lawa , N. M. P. Setyani , Y. Y. Bette , A. N. Banamtuan, and R. M. J. Jami Riwu
Keywords: oxidative stress, Computer-assisted sperm analysis, extender supplementation, semen preservation, sperm kinematics.
The decrease in the sperm kinematic quality during cold storage is still a significant limitation in the artificial insemination programs, especially on local breeds like the Rote sheep. This experiment was an attempt to determine the impacts of adding Bovine Serum Albumin (BSA), a protein derived from bovine blood plasma that functions as an antioxidant and stabilizer in biological media, and Moringa leaf extract (MLE), a natural extract from Moringa oleifera leaves rich in antioxidants, vitamins, and bioactive compounds, to Tris-based extender on the kinematic properties of Rote ram sperms at 5oC in 48 hours. Two healthy Rote rams (2-3 years old) were collected with an electroejaculator, and semen was evaluated macroscopically and microscopically. Processing was done on ejaculates whose progressive motility was ≥70%, and sperm abnormalities were less than 20%. In the control (P0), the semen samples were diluted with Tris-egg yolk extender; supplementation was made with 0.3% BSA (P1), 0.125% MLE (P2), 0.3% BSA + 0.125% MLE (P3), 0.3% BSA + 0.25% MLE (P4), 0.45% BSA + 0.125% MLE (P5), and 0.45% BSA + 0.25% MLE (P6). Diluted semen samples were stored in Eppendorf tubes and maintained at 5°C in a refrigerator for up to 48 hours. The Computer-Assisted Sperm Analysis (CASA; an automated and objective system used to evaluate sperm motility and kinematic characteristics through digital image processing) was used to determine sperm motility and kinematic parameters. It was established that treatment P3 had the highest and significantly different (p<0.05) results in the majority of the parameters, such as progressive motility (52.80%), fast motility (27.31%), VCL (curvilinear velocity, 105.85 µm/s), VSL (straight-line velocity, 41.55 µm/s), VAP (average path velocity, 49.55 µm/s), and STR (straightness ratio, 0.36). Finally, it was found that Tris extender supplemented with 0.3% BSA and 0.125% MLE was effective in preserving sperm kinematic quality of Rote ram semen throughout 48 hours of cold storage.
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