JOURNAL OF ANIMAL SCIENCE AND VETERINARY MEDICINE
Integrity Research Journals
ISSN: 2536-7099
Model: Open Access/Peer Reviewed
DOI: 10.31248/JASVM
Start Year: 2016
Email: jasvm@integrityresjournals.org
Cryoprotective potential of honey in caprine semen extension
https://doi.org/10.31248/JASVM2025.564 | Article Number: C683F0119 | Vol.10 (3) - June 2025
Received Date: 23 April 2025 | Accepted Date: 16 June 2025 | Published Date: 30 June 2025
Authors: O. Alaba* , J. O. Ogundipe and F. P. Agbaye
Keywords: glycerol, fertilising ability, cryopreservation, semen quality, goats
The study was carried out to determine the effect of honey as a cryoprotectant agent in caprine semen cryopreservation to improve the viability of frozen-thawed caprine semen. The semen used was collected from two healthy and proven mature bucks weighing 30 to 35 kg using an electro-ejaculator. The semen after collection was divided into five groups to form five different treatments, with the control as T1, containing only glycerol. T2, T3, T4, and T5 contained 25%, 50%, 75%, and 100% honey, respectively, and were stored in a freezer at a temperature of -18 to -20°C. After dilution and freezing, the frozen semen was assessed for livability, mass activity, morphology, plasma membrane, acrosome integrity, and pH at 24-hour intervals for four days. Each sample was thawed in a water bath at 37 oC for 60 seconds before evaluation. The effect of honey and storage day on livability, morphology, plasma membrane, acrosome integrity, and pH was significantly affected (p>0.05) but fell within a normal range for caprine semen viability. With increasing storage days, there was a progressive slight decrease in all the parameters measured. The result showed that including honey in cryopreservation, the diluent had no deleterious effect on the quality parameters of caprine semen. It was concluded that honey has a promising ingredient in semen extension as it is not detrimental to the quality parameters of semen after storage for up to 72 hours.
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