Integrity Research Journals

ISSN: 2536-7099
Model: Open Access/Peer Reviewed
DOI: 10.31248/JASVM
Start Year: 2016

Anthelmintic effects of four medicinal plants using in vitro L3 larva stage   |   Article Number: B86A01035   |   Vol.7 (3) - June 2022

Received Date: 19 May 2022   |   Accepted Date: 13 June 2022  |   Published Date: 30 June 2022

Authors:  Bada, A. A.* , Osho, I. B. , Adewole, S. O. and Olofintoye, L. K.

Keywords: Health, medicinal plants, Anthelminthic, larva

In vitro anthelminthic activity of Vernonia amygdalina, Ocimum grattissimum, Nicotiana tabacum and Talinum triangulare using L3 stage larva was investigated. Standard procedure was followed for larva culturing, preparation of extract and for in vitro assay. The concentration of Vernonia amygdalina was effective at 1.0 mg/ml at 1 and 2 hours, Ocimum grattissimum at 0.5 mg/ml at 1 hour, Nicotiana tabacum at 0.125 mg/ml at 1 hour and Talinum triangulare at 0.125 mg/ml at 2 hours. The result showed that there was anthelminthic activity in the four plants after 3 hours of the experiment. Nicotiana tabacum showed a faster anthelmintic effect at a lower concentration of 0.125 mg/l and at a lower hour (1 hour) while Talinum triangulare showed a slower anthelmintic effect compared to other medicinal plants at 2 hours. The health status of ruminants can be improved through the consumption of these four medicinal plants.

Adeola, A. O., Adewole, S. O., & OLofintoye, L. K. (2015a). Phytochemical screening of the four different medicinal plants. Unique Research Jouranl of Agricultural Sciences, 3(1), 1-6.
Adeola, A. O., Osho, I. B., Adewole, S. O., & OLofintoye, L. K. (2015b). In vivo effects of four medicinal plants on nematodes of goat. Journal of Biology and Nature, 4(2), 122-126.
Adeola, A. O., Osho, I. B., Adewole, S. O., & Olofintoye, L. K. (2016). In vitro anthelmintic effects of three medicinal plants. Journal of Health Sciences and Nursing, 2(7), 55-63.
Alawa, C. B. I., Adamu, A. M., Gefu, J. O., Ajanusi, O. J., Abdu, P. A., Chiezey, N. D., Alawa, J. N., & Bowman , D. D. (2003). In vitro screening of two Nigerian medicinal plants Vernonia amygadalina and Annoa senegalensis for anthelmintic activity. Veterinary Parasitology. 113(1), 73-81.
Athanasiadou, S., Kyriazakis, I., Jackson, F., & Coop, R. L. (2001). Direct anthelmintic effects of condensed tannins towards different gastrointestinal nematodes of sheep: in vitro and in vivo studies. Veterinary parasitology, 99(3), 205-219.
Dawson, J. M., Buttery, P. J., Jenkins, D., Wood, C. D., & Gill, M. (1999). Effects of dietary quebracho tannin on nutrient utilisation and tissue metabolism in sheep and rats. Journal of the Science of Food and Agriculture, 79(11), 1423-1430.
Edeoga, H. O., Okwu, D. E., & Mbaebie, B. O. (2005). Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology, 4(7), 685-688.
Edegbai, B. O., & Anoliefo, G. O. (2019). Growth and development of Bitter leaf (Vernonia amygdalina Del.) in soils treated with mixture of cadmium and lead. Journal of Applied Sciences and Environmental Management, 23(5), 835-841.
Iqbal, Z., Nadeem, Q. K., Khan, M. N., Akhtar, M. S., & Waraich, F. N. (2001). In vitro anthelmintic activity of Allium sativum, Zingiber officinale, Curcurbita mexicana and Ficus religiosa. International Journal of Agriculture and Biology, 3(4), 454-457.
Iwalewa, E. O., Adewunmi, C. O., Omisore, N. O. A., Adebanji, O. A., Azike, C. K., Adigun, A. O., ... & Olowoyo, O. G. (2005). Pro-and antioxidant effects and cytoprotective potentials of nine edible vegetables in southwest Nigeria. Journal of Medicinal Food, 8(4), 539-544.
Nalule, A. S., Mbaria, J. M., & Kimeju, J. W., (2013). In vitro anthelmintic potential of Vernonia amygdalina and Secamore africana on gastrointestinal nematodes. Agriculture and Biology Journal of North America, 4(1), 254-266.
Pessoa, L. M., Morais, S. M., Bevilaqua, C. M., & Luciano, J. H. S. (2002). Antihelmintic activity of essential oil of Ocimum grattissimum Lin. And eugneol against Haemonthus contortous. Veterinary. Parasitology, 109(1-2), 59-63.
Rates, S. M. K. (2001). Plants as source of drugs. Toxicon, 39(5), 603-613.
Satou, T., Koga, M., Matsuhashi, R., Koike, K., Tada, I., & Nikaido, T. (2002). Assay of nematocidal activity of isoquinoline alkaloids using third-stage larvae of Strongyloides ratti and S. venezuelensis. Veterinary Parasitology, 104(2), 131-138.
Van Wyk, J. A., & Mayhew, E. (2013). Morphological identification of parasitic nematode infective larvae of small ruminants and cattle: A practical lab guide. Onderstepoort Journal of Veterinary Research, 80(1), 1-14.
Iqbal, Z., Lateef, M., Jabbar, A., Ghayur, M. N., & Gilani, A. H. (2006). In vitro and in vivo anthelmintic activity of Nicotiana tabacum L. leaves against gastrointestinal nematodes of sheep. Phytotherapy Research, 20(1), 46-48.