Journal of Animal Science and Veterinary Medicine

JOURNAL OF ANIMAL SCIENCE AND VETERINARY MEDICINE
Integrity Research Journals

ISSN: 2536-7099
Model: Open Access/Peer Reviewed
DOI: 10.31248/JASVM
Start Year: 2016
Email: jasvm@integrityresjournals.org

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Evaluation of Lawsonia inermis (Henna) and Hibiscus sabdariffa (Roselle) ethanol extracts as substitute for Eosin stain in Histology using Albino rats

https://doi.org/10.31248/JASVM2024.455   |   Article Number: A6EEC6197   |   Vol.9 (4) - August 2024

Received Date: 07 July 2024   |   Accepted Date: 30 July 2024  |   Published Date: 30 August 2024

Authors:  Barde Israel Joshua , Samson Blessing Oshe , Arieri Onikisateinba , Umaru Nendir John Haruna , Shekaro Audu , Bakam Judith Dizot , Umaru Mada Alesa , Mafulul Bako Joshua , Obabofa Omiela Victoria , Govina Folakemi Justina , Diepreye Tekenah Ebierinyu , Olabode Mayowa Peter , Akaehielem Glory Chidera , Darlington Kate Ogochuwkwu and Ishaku Leo Elisha

Keywords: Eosin substitute, Hibiscus sabdariffa extract, histology stains, Lawsonia inermis extract.

ABSTRACT

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Staining techniques have significantly advanced the identification of tissue structures under the microscope, facilitating precise diagnostic processes. Stains, which are dyes modified for biological use, are essential in histological studies. This study aims to assess the staining efficacy of ethanol extracts from Lawsonia inermis (Henna) and Hibiscus sabdariffa (Roselle) compared to the traditional eosin stain. Various ethanol extract solutions of L. inermis and H. sabdariffa leaves were prepared at concentrations of 20 and 50%. These solutions were further modified by incorporating aluminium sulphate as a mordant, applied at intervals of 5 minutes (20%) and 10 minutes (50%). The acidity of these solutions, including those with mordants, was confirmed. Histological sections of the lungs, liver, kidney, intestine, and heart were stained using Hematoxylin and Eosin as a control, alongside the modified ethanol extract solutions as counterstains. The extracts stained the cytoplasm, yielding a brownish hue, with no significant differences observed between the photomicrographs of cells stained with pure and mordant solutions when compared with the control group. These findings showed that Henna and Roselle extracts could serve as viable substitutes for eosin stain due to their acidic properties, appropriate staining times, of 5 and 10 minutes, and lower toxicity. Further research that will address the clarity of the stains was recommended.

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