JOURNAL OF ANIMAL SCIENCE AND VETERINARY MEDICINE
Integrity Research Journals

ISSN: 2536-7099
Model: Open Access/Peer Reviewed
DOI: 10.31248/JASVM
Start Year: 2016
Email: jasvm@integrityresjournals.org


Influence of brine NaCl concentration and brining time on the physicochemical, microbiological and sensory properties of pork

https://doi.org/10.31248/JASVM2025.685   |   Article Number: 86127C472   |   Vol.11 (5) - October 2026

Received Date: 19 August 2026   |   Accepted Date: 24 September 2026  |   Published Date: 16 August 2026

Authors:  Ekpo, J. S. , Sam, I. M. , Enobong, O. Umoh and Udo, A. F.*

Keywords: sensory evaluation, meat quality, Curing, microbial stability, Sodium chloride.

Brining is an important meat-processing technique that can modify moisture distribution, technological properties, microbial stability and sensory quality. This study evaluated the effects of NaCl concentration and brining time on the physicochemical, microbiological and sensory properties of pork. Fresh pork was subjected to a 3 × 2 factorial arrangement involving 30, 60 and 90 min brining periods and 10 and 15% NaCl concentrations. Brining time increased salt uptake but was associated with greater drip loss and reduced water-holding capacity. The higher NaCl concentration promoted greater brine uptake but also increased drip loss and shear force. Brining markedly reduced the loads of Staphylococcus, Escherichia and Salmonella, with the strongest reductions generally occurring at longer exposure periods. Sensory evaluation showed improved colour, tenderness, flavour, juiciness and overall acceptability at 60 and 90 min compared with 30 min. The responses indicate that brining time and NaCl concentration should be considered jointly because greater salt uptake did not necessarily correspond with improved technological properties. Under the conditions of the present study, 15% NaCl for 60 min provided a favourable compromise between physicochemical characteristics, microbial suppression and sensory acceptability.

Adediran, O. A., Badmus, F., Ekpo, J. S., & Okon, U. M. (2025). Qualitative indices of chicken nuggets as affected by different cooking methods. Ethiopian Journal of Public Health and Nutrition, 8(1), 24.
 
Alphonsus, C., Finangwai, H. I., Yashim, S. M., Agubosi, O. C. P., & Sam, I. M. (2010). Effect of dam parity on measures of growth in red sokoto goats 1, 3, 6 and month of age. Continental J. Animal and Veterinary Research, 2, 9-13.
 
Alphonsus, C., Sam, I. M., Opoola, E., & Ayigun, A. E. (2012). Correlated relationship amongst performance and carcass characteristics in broiler chicken raised to maturity. Animal Production Research Advances, 8(1), 50-53.
 
Assam, E. M., Ndak, U. A., & Udo, A. F. (2026a). Haemato-serological indices of weaner pigs fed fermented and pressed Icacina mannii in the humid tropics. AKSU Journal of Agriculture and Food Science, 10(2).
 
Assam, E. M., Ukpanah, U. A., Ndak, U. U., Udo, A. F., & Green, B. S. (2026b). Influence of multi-enzyme (Liptozyme®) addition on performance and haematological parameters of growing pigs fed diets with fermented Icacina mannii meal. Journal of Animal Science and Veterinary Medicine, 11(3), 196-201.
 
Christopher, G. I., Usoro, O. O., Sam, I. M., & Udoudo, U. S. (2024). Effect of feeding graded levels of sun-dried Moringa oleifera leaf meal (MOLM) on the reproductive performance of cross-bred rabbit does in Southern Nigeria. AKSU Journal of Agriculture and Food Sciences, 8(2), 185-194.
https://doi.org/10.61090/aksuja.2024.028
 
Desmond, E. (2006). Reducing salt: A challenge for the meat industry. Meat Science, 74(1), 188-196.
https://doi.org/10.1016/j.meatsci.2006.04.014
 
Ekpo, J. S., Etuk, I. F., Evoh, G. D., & Obasi, O. I. (2008). Effects of dietary three sundried cassava feed forms on the performance of weaner rabbits. Nigerian Journal of Agricultural Technology, 13, 16-21.
 
Ekpo, J. S., Sampson, S. O., Eyoh, G. D., & Sam, I. M. (2018). Comparative evaluation of the effects of three local spices on nutrient and organoleptic qualities of rabbit meat. Nigerian Journal of Animal Production, 45(5), 107-111.
https://doi.org/10.51791/njap.v45i5.244
 
Essien, C. A., & Sam, I. M. (2018). Effect of Anthonotha macrophylla (African beans) leaf meal on the blood profiles of finishing broiler chickens. International Journal of Agriculture: Research and Review, 6(1), 43-48.
 
Essien, C. A., Sam, I. M., & Okon, U. M. (2023). Effect of Jatropha tanjorensis leaf meal on performance, carcass and internal organ characteristics of broiler finisher chickens. In Proceedings of the 28th Annual Conference of the Animal Science Association of Nigeria (pp. 403-406). Animal Science Association of Nigeria.
 
FAO. (2025). OECD-FAO agricultural outlook 2025-2034. OECD Publishing.
 
Gil, M., Rudy, M., Duma-Kocan, P., Stanisławczyk, R., Wolińska, A., Krajewska, A., & Dziki, D. (2025). Effect of marinating on selected quality characteristics of pork tenderloin cooked by sous vide method. Foods, 14(11), 1958.
https://doi.org/10.3390/foods14111958
 
Inguglia, E. S., Zhang, Z., Tiwari, B. K., Kerry, J. P., & Burgess, C. M. (2017). Salt reduction strategies in processed meat products-A review. Trends in Food Science & Technology, 59, 70-78.
https://doi.org/10.1016/j.tifs.2016.10.016
 
Jeong, K., O, H., Shin, S., & Kim, Y.-S. (2018). Effects of Different Marination Conditions on Quality, Microbiological Properties, and Sensory Characteristics of Pork Ham Cooked by the Sous-vide Method. Korean Journal for Food Science of Animal Resources, 38, 506-514.
 
Jin, G., Liu, Y., Zhang, Y., Li, C., He, L., Zhang, Y., Wang, Y., & Cao, J. (2023). Underlying formation mechanisms of ultrasound-assisted brined porcine meat: The role of physicochemical modification, myofiber fragmentation and histological organization. Ultrasonics Sonochemistry, 94, 106318.
https://doi.org/10.1016/j.ultsonch.2023.106318
 
Khayrullin, M., Curcan, A. V., Nesterenko, A., Zhuravlev, M., & Rystakov, A. V. (2021). Results of studies of samples of boiled-smoked loin with different injection levels. IOP Conference Series: Earth and Environmental Science, 677.
https://doi.org/10.1088/1755-1315/677/3/032014
 
Li, H., Zhi, Y., Nie, Y., Liu, J., Liao, Z., & He, X. (2024). The impact of barbecue pickling materials on flavor changes and storage quality of pork at different processing stages. International Journal of Food Engineering, 20, 531-539.
https://doi.org/10.1515/ijfe-2024-0004
 
Lonergan, S. M., Huff-Lonergan, E., Rowe, L. J., Kuhlers, D. L., & Jungst, S. B. (2001). Selection for lean growth efficiency in Duroc pigs influences pork quality. Journal of Animal Science, 79(8), 2075-2085.
https://doi.org/10.2527/2001.7982075x
 
Lu, J., Li, R., Chen, H., Sun, D., Yu, Z., Liu, Y., Zhang, B., & Jiang, W. (2024). Effect of brine concentration on the quality of salted large yellow croaker during processing and refrigeration. Food Science and Biotechnology, 33, 3257-3267.
https://doi.org/10.1007/s10068-024-01573-5
 
Ozuna, C., Puig, A., García-Pérez, J. V., Mulet, A., & Cárcel, J. (2013). Influence of high intensity ultrasound application on mass transport, microstructure and textural properties of pork meat (Longissimus dorsi) brined at different NaCl concentrations. Journal of Food Engineering, 119, 84-93.
https://doi.org/10.1016/j.jfoodeng.2013.05.016
 
Rahman, S., Sharmeen, I., Pan, J., Kong, D., Xi, Q., Du, Q., Yang, Y., Wang, J., Oh, D., & Han, R. (2023). Marination ingredients on meat quality and safety - a review. Food Quality and Safety, 7, fyad027.
https://doi.org/10.1093/fqsafe/fyad027
 
Sam, I. M., Ekanem, J. T., & Udo, A. F. (2026a). Sex-age interactions, carcass characteristics, and logarithmic regression modelling in broiler chickens. AKSU Journal of Agriculture and Food Sciences, 10(1), 65-77.
 
Sam, I. M., Udo, A. F., & Oloruntobi, E. A. (2026b). Predictive power and enhancing body weight estimation in sheep: A review. AKSU Journal of Agriculture and Food Sciences, 10(2), 112-120
 
Sam, I. M., Ukpanah, U. A., & Udofia, I. E. (2020). Influence of genotype on body weight and morphometric traits of rabbits raised in the tropics. Animal Research International, 17(1), 3603.
 
Siroli, L., Baldi, G., Soglia, F., Bukvicki, D., Patrignani, F., Petracci, M., & Lanciotti, R. (2020). Use of essential oils to increase the safety and the quality of marinated pork loin. Foods, 9(8), 987.
https://doi.org/10.3390/foods9080987
 
Udo, A. F., Sam, I. M., & Udo, M. D. (2025a). Evaluation and correlation of egg quality traits between Isa Brown and Nera Black commercial layer strains. Animal Research International, 22(3), 6472-6482
 
Udo, A. F., Sam, I. M., Udo, M. D., & Ekpo, J. S. (2025b). Comparative evaluation of reproductive and egg production performance of two strains of layer chicken raised in the humid tropics. Abuja Journal of Agriculture and Environment, 5(2), 262-270.
 
Udo, M. D., Jeremiah, J. I., Udo, A. F., Eyoh, G. D., & Okon, L. S. (2024). Growth indices and blood profile of West African dwarf goats fed processed earth ball (Icacina manni) meal based diets. AKSU Journal of Agriculture and Food Sciences, 8(3), 136-146.
 
Udoudo, U. S., Ekpo, J. S., Sam, I. M., & Okon, L. S. (2025). Meat quality, serum and meat lipid profiles of broiler chicken fed Persea americana, Jatropha tanjorensis and Ocimum basilicum leaves extract via drinking water. Journal of Animal Science and Veterinary Medicine, 10(5), 441-446.
https://doi.org/10.31248/JASVM2025.605
 
Visy, A., Jonas, G., Szakos, D., Horváth-Mezőfi, Z., Hidas, K. I., Barko, A., & Friedrich, L. (2021a). Evaluation of ultrasound and microbubbles effect on pork meat during brining process. Ultrasonics Sonochemistry, 75, 105589.
https://doi.org/10.1016/j.ultsonch.2021.105589
 
Visy, A., Leong, S. Y., & Oey, I. (2021b). Effects of ultrasound and microbubbles on salt diffusion in pork meat. Ultrasonics Sonochemistry, 75, 105589.
https://doi.org/10.1016/j.ultsonch.2021.105589
 
Xu, X., Chen, X., Chen, D., Yu, B., Yin, J., & Huang, Z. (2019). Effects of dietary apple polyphenol Xu, X., Chen, X., Chen, D., Yu, B., Yin, J., & Huang, Z. (2019). Effects of dietary apple polyphenol supplementation on carcass traits, meat quality, muscle amino acid and fatty acid composition in finishing pigs. Food & Function, 10(11), 7426-7434.
https://doi.org/10.1039/C9FO01304K
 
Yang, H.-S., Kang, S.-W., Joo, S., & Choi, S. (2012). Effects of Salt Concentration and Drying Time on the Quality Characteristics of Pork Jerky during Dehydration. Korean Journal for Food Science of Animal Resources, 32, 285-292.
https://doi.org/10.5851/kosfa.2012.32.3.285
 
Yu, M., Li, Z., Rong, T., Wang, G., Liu, Z., Chen, W., Li, J., Li, J., & Ma, X. (2020). Different dietary starch sources alter the carcass traits, meat quality, and the profile of muscle amino acid and fatty acid in finishing pigs. Journal of Animal Science and Biotechnology, 11(1), 78.
https://doi.org/10.1186/s40104-020-00484-9