ISSN: 2536-7099
Model: Open Access/Peer Reviewed
DOI: 10.31248/JASVM
Start Year: 2016
Email: jasvm@integrityresjournals.org
https://doi.org/10.31248/JASVM2026.663 | Article Number: 7C44773E6 | Vol.11 (3) - June 2026
Received Date: 04 May 2026 | Accepted Date: 12 June 2026 | Published Date: 30 June 2026
Authors: Assam, Ekeno Michael , Ukpanah, Unwana Anietie , Ndak, Uwem Udo , Udo, Akaninyene Friday* and Green, Blessing Samuel
Keywords: haematology, serum, Growing pigs, Icacinia mannii, Liptozyme.
The increasing cost and scarcity of maize in pig production necessitate the exploration of alternative energy feed resources such as fermented and pressed Icacina mannii meal (FPIM). A study was conducted to evaluate the effect of multi-enzyme (Liptozyme) supplementation on growth performance, blood profile, and cost benefits of growing pigs fed diets containing fermented and pressed Icacina mannii meal (FPIM). Twelve crossbred growing pigs with initial weights of 26–28 kg were randomly allotted to four dietary treatments in a completely randomised design with three replicates per treatment. Fermented and pressed Icacina mannii replaced maize at 0%, 10%, 20%, and 30% levels in diets designated as T1, T2, T3, and T4, respectively. Diets T2–T4 were supplemented with Liptozyme at 1 g/kg feed, while T1 served as the control. Data were analysed using one-way ANOVA and Duncan’s multiple range test. Results showed that the control diet (T1) recorded the highest final body weight (50523.33 g), which was significantly superior (p<0.05) to the other treatments. Final weights for T2 and T3 were statistically similar (43733.33 g and 43500.00 g, respectively), while T4 had the lowest value. Feed intake followed a similar trend, while T4 recorded the lowest feed conversion ratio. Haematological indices were not negatively affected (P>0.05) by dietary treatments. In conclusion, enzyme supplementation reduced feed cost, but the inclusion of FPIM at higher levels, particularly 30%, was not economically beneficial.
| Amaefule, R. A., Etuk, I. F., Iwuji, T. C., Ogbuewu, I. P., Obikaonu, O. H., & Amaefule, K. U. (2020). Haematological indices of grower pigs fed low protein and low energy diets supplemented with multi-enzyme. Nigerian Journal of Animal Production, 47(1), 167-173. https://doi.org/10.51791/njap.v47i1.226 |
||||
| Assam, E M. (2014). The performance of broiler birds fed raw or processes (Earth-ball) Icacina mannii meal. M.Sc Thesis. Micheal Okpara University of Agriculture, Umudike, Abia State, Nigeria. | ||||
| Assam, E. M. (2023). Chemical and nutritional evaluation of Icacina manni (earth ball) meals in the diet of weaner pigs. Ph.D Dissertation submitted to the Department of Animal Production and Livestock Management, Michael Okpara University of Agriculture, Umudike. | ||||
| Essien, C. A., & Sam, I. M. (2018). Nutritional evaluation of Icacinia manni (Earth Ball) processed in saline as a source of dietary energy in broiler production. Journal of Biology, Agriculture and Healthcare, 8(2), 19-23. | ||||
| Etim, N. N., Enyenihi, G. E., Williams, M. E., Udo, M. D., & Offiong, E. E. (2013). Haematological parameters: indicators of the physiological status of farm animals. British Journal of Science, 10(1), 33-45. | ||||
| Fasuyi, A. O., Ibitayo, F. J., & Alo, S. O. (2013). Histopathology, haematology and serum chemistry of growing pigs fed varying levels of wild sunflower (Tithonia diversifolia) leaf meal as protein supplements. Journal of Agriculture and Veterinary Science, 4(1), 41-50. https://doi.org/10.9790/2380-0414150 |
||||
| Hahn, S. K., Mahungu, N. M., Otoo, J. A., Msabaha, M. A. M., Lutaladio, N. B., & Dahniya, M. T. (1987, August). Cassava and African food crisis. In Tropical Root Crops at the African Food Crisis". Proceedings of the 3rd Triennial Symposium of the International Society for Tropical Root Crops. African Branch. Owerri, Nigeria. (Editors: Terry, ER, Akoroda, MO and Arene, OB). IDRC-258e Publ. Canada. Pg (pp. 24-29). | ||||
| Jelek, J., Stark, J., Nemec, M., Plut, J., Oven, I. G., Klinkon, M., & Stukelj, M. (2018). The influence of age, farm, and physiological status on pig hematological profiles. Journal of Swine Health and Production, 26(2), 72-78. https://doi.org/10.54846/jshap/1049 |
||||
| Makanjuola, O. M., Ogunmodede, A. S., Makanjuola, J. O., & Awonorin, S. O. (2012). Comparative study on quality attributes of gari obtained from some processing centres in South West, Nigeria. Advance Journal of Food Science and Technology, 4(3), 135-140. | ||||
| Mapcarta (2026). Oruk Anam is a Local Government Area. Retrieved from https://mapcarta.com/27748444. | ||||
| Niemi, P., Aura, A. M., Maukonen, J., Smeds, A. I., Mattila, I., Niemelä, K., Tamminen, T., Faulds, C.B., Buchert, J., & Poutanen, K. (2013). Interactions of a lignin-rich fraction from brewer's spent grain with gut microbiota in vitro. Journal of Agricultural and Food Chemistry, 61(27), 6754-6762. https://doi.org/10.1021/jf401738x |
||||
| Norachack, B., Keonouchanh, S., Ty, C., Bouahom, B., & Preston, T. R. (2004). Stylosanthes and cassava leaves protein supplements to a basal diet of broken rice for local pigs. Livestock Research for Rural Development, 16(10). | ||||
| Rahman, A., Pasha, T. N., Younus, M., Abbas, Y., & Ditta, Y. A. (2014). Effect of multi-enzymes supplementation on growth performance of broiler. Pakistan Journal of Zoology, 46(2), 417-422. | ||||
| Shipman, G. L., Perez-Palencia, J. Y., Rogiewicz, A., Patterson, R., & Levesque, C. L. (2023). Evaluation of multienzyme supplementation and fiber levels on nutrient and energy digestibility of diets fed to gestating sows and growing pigs. Journal of Animal Science, 101, skad375. https://doi.org/10.1093/jas/skad341.207 |
||||
| Shirmohammad, F., & Mehri, M. (2011). Effects of dietary supplementation of multi-enzyme complex on the energy utilization in rooster and performance of broiler chicks. African Journal of Biotechnology, 10(38), 7541-7547. | ||||
| Song, M., Kim, B., Cho, J. H., Kyoung, H., Park, S., Cho, J. Y., Park, K. I., Kim, H. B., & Lee, J. J. (2022). Effects of dietary protease supplementation on growth rate, nutrient digestibility, and intestinal morphology of weaned pigs. Journal of Animal Science and Technology, 64(3), 462. https://doi.org/10.5187/jast.2022.e28 |
||||
| Steel, R.G. D and Torrie, J.H. (1980). Principles and procedures of statistics. McGraw-Hill Co. Inc. New York. | ||||
| Tram, N. D. Q., & Preston, T. R. (2004). Effect of method of processing cassava leaves on intake, digestibility and N retention by Ba Xuyen piglets. Livestock Research for Rural Development, 16(10). | ||||
| Udedibie, A. B. I., Anyaegbu, B. C., Onyechekwa, G. C., & Egbuokporo, O. C. (2004). Effect of feeding different levels of fermented and unfermented cassava tuber meals on performance of broilers. Nigerian Journal of Animal Production, 31, 211-219. https://doi.org/10.51791/njap.v31i2.1816 |
||||
| Udo, M. D., Jeremiah, J. I., Udo, A. F., Eyoh, G. D., & Okon, L. S. (2024). Growth indices and blood profile of West African Dwarf goats fed processed earth ball (Icacina manni) meal based diets. AKSU Journal of Agriculture and Food Sciences, 8(3) 136-146 | ||||
| Udom, I., Nta, S., Usoh, G., Kamai, M., & Ugwuishiwu, B. (2024). Rainfall-groundwater table fluctuations Impact on Root zone soil water simulated in Upflow for crop production planning in obio akpa watershed, Nigeria. Journal of Digital Food, Energy & Water Systems, 5(1), 88-103. https://doi.org/10.36615/995gzf79 |
||||
| Valente Junior, D. T., Genova, J. L., Kim, S. W., Saraiva, A., & Rocha, G. C. (2024). Carbohydrases and phytase in poultry and pig nutrition: a review beyond the nutrients and energy matrix. Animals, 14(2), 226. https://doi.org/10.3390/ani14020226 |
||||
| Wang, X., Ma, R., Xie, X., Liu, W., Tu, T., Zheng, F., You, S., Ge, J., Xie, H., Yao, B., & Luo, H. (2017). Thermostability improvement of a Talaromyces leycettanus xylanase by rational protein engineering. Scientific Reports, 7, 15287. https://doi.org/10.1038/s41598-017-12659-y |
||||