JOURNAL OF ANIMAL SCIENCE AND VETERINARY MEDICINE
Integrity Research Journals

ISSN: 2536-7099
Model: Open Access/Peer Reviewed
DOI: 10.31248/JASVM
Start Year: 2016
Email: jasvm@integrityresjournals.org


Haematology, blood viscosity and osmotic fragility of finisher broiler chickens fed dietary supplementation of acetylsalicylic acid during hot dry season

https://doi.org/10.31248/JASVM2024.440   |   Article Number: 7A67D1781   |   Vol.9 (3) - June 2024

Received Date: 06 May 2024   |   Accepted Date: 20 June 2024  |   Published Date: 30 June 2024

Authors:  Olusola Olufisayo Awoneye* , Aruna Imoru and Sehinde Samson Babadipe

Keywords: haematology, Broiler, Acetylsalicylic acid, fragility, viscosity.

The effect of acetylsalicylic acid on the haematological status, blood viscosity and osmotic fragility of broiler chickens were evaluated during transition from dry to rainy season (January – March). One hundred and ninety two (192) day old broiler chicks (ANAK) were randomly divided into four dietary treatments, each treatment comprising 48 birds, which was replicated 4 times containing 12 birds per replicate. Four experimental diets were formulated to contain acetylsalicylic acid (ASA) at 0, 0.025, 0.050 and 0.075 percentage; the diets were formulated to meet the nutrient requirement of the birds. All data collected were subjected to one-way analyses of variance (ANOVA) using a completely randomized design. The result of the hematological study showed that the packed cell volume, red blood cell, erythrocyte sedimentation rate, mean cell volume, mean cell haemoglobin and heretrophil/lymphocyte ratio were influenced by the treatment diets. Broilers fed 0.075% ASA had the highest packed cell volume and red blood cell values of 29.63±0.77% and 2.60±0.17×106 mm respectively. The blood viscosity study revealed that the whole blood viscosity decreased as level of ASA supplementation increased. Broilers fed 0.050% ASA had the lowest (p<0.05) serum and plasma viscosity values of 1.05±0.03 cP and 0.72±0.01 cP, respectively. Statistical variations (p<0.05) were observed at all levels of saline concentration across the treatment, with broilers fed 0.075% having the highest number of osmotically stable red blood cells across the treatments at all saline concentrations. It could be concluded that birds fed ASA supplemental diets were less viscous, osmotically stable and had a better haematological profile than the control diet.

Adenkola, A. Y., & Ayo, J. O. (2009). Effect of Ascorbic acid on rectal temperature fluctuations in indigenous Turkeys during the hot-dry season. International Journal of Poultry Science, 8(5), 457-461
https://doi.org/10.3923/ijps.2009.457.461
 
Adenkola, A. Y., Ayo, J. O., Sackey, A. K. B., & Adelaiye, A. B. (2010). Erythrocyte osmotic fragility of pigs administered ascorbic acid and transported by road for short term duration during the harmattan season. African Journal of Biotechnology, 9(2), 226-233.
 
Aengwanich, W. (2008). Pathological changes and the effects of ascorbic acid on lesion scores of bursa of Fabricius in broilers under chronic heat stress. Research Journal of Veterinary Science, 1(1), 62-66.
https://doi.org/10.3923/rjvs.2008.62.66
 
Aldrich, K. J., Saunders, D. K., Sievert, L. M., & Sievert, G. (2006). Comparison of erythrocyte osmotic fragility among amphibians, reptiles, birds and mammals. Transactions of the Kansas Academy of Science, 109(3), 149-158.
https://doi.org/10.1660/0022-8443(2006)109[149:COEOFA]2.0.CO;2
 
Al-Obaidi, F. A., & Al-Shadeedi, S.M. (2010). Effect of dietary aspirin for reducing ascites and enhancing productive performance of broilers reared in high density. AL-Qadisiyah Journal of Veterinary Medicine Sciences, 9(1), 20-25.
 
Amao, E. A., & Siyanbola, M. F. (2012). Effect of dietary acetylsalicylic acid in alleviating heat stress in laying birds in the tropics. Polytechnic Journal of Science and Technology, 6, 9-14.
 
Aro, S. O., & Akinleminu, S. B. (2015). Blood viscosity and osmotic fragility of late production layers on different dietary salt levels. Proceedings of the 8th Annual Agricultural Conference of the School of Agriculture and Agricultural Technology, FUTA, Akure. Pp. 151-155.
 
Aro, S. O., Baki O. I. & Awoneye, O. O. (2017b). Osmotic fragility, circulating luitenizing and steroid hormones in laying chickens fed varying dietary levels of acetylsalicylic acid. Proceedings of 42nd Annual Conference Nigeria Society for Animal Production (NSAP). Landmark University, Omu- Aran, Kwara State. Pp. 192-195.
 
Aro, S. O., Osho, I. B., & Awoneye, O. O. (2017a). Comparison of rectal and axillary temperatures of Isa Brown and Harco Black layers fed different levels of dietary acetylsalicylic acid. Animal Research International, 14(1), 2691-2696.
 
Balog, J. M., Huff, G. R., Rath, N. C., & Huff, W. E. (2000). Effect of dietary aspirin on ascites in broilers raised in a hypobaric chamber. Poultry Science, 79(8), 1101-1105.
https://doi.org/10.1093/ps/79.8.1101
 
Bartfai, T., & Conti, B. (2010). Fever. The Scientific World Journal, 10, 490-503.
https://doi.org/10.1100/tsw.2010.50
 
Bartlett, J. R., & Smith, M. O. (2003). Effects of different levels of zinc on the performance and immunocompetence of broilers under heat stress. Poultry Science, 82(10), 1580-1588.
https://doi.org/10.1093/ps/82.10.1580
 
Bode-Böger, B. (2005). Aspirin (acetylsalicylic acid) is the most widely prescribed non-steroidal anti- inflammatory drug, and is used in the cure and anticipation of normal cardiovascular disorders. European Journal of clinical Investigation, 26,747-754.
 
Campbell, T. W., & Ellis, C. K. (2007). Haematology of birds. In: Campbell, T. W., & Ellis, C. K. (eds.). Avian and exotic animal haematology and cytology (Third edition.). Blackwell Publishing Professional, Ames (IA). Pp. 3-50.
 
Climatic Data (2018). National Centre for Environmental Information. State of the climate: Global climate report for annual 2018. Retrieved from https://www.ncei.noaa.gov/ access/monitoring/monthly-report/global/201813.
 
Drabkin, D. I. (1945). Crystallographic and optical properties of human hemoglobin. A proposal for the standarization of hemoglobin. American Journal of Medicine and Surgery, 209, 268-270.
 
Laura, S., Naomide, B., & Marc, R. (2009). Dietary beta glucans to fight chronic enteritis. World Poultry, 25(12), 14-16.
 
Marketon, J. I. W., & Glaser, R. (2008). Stress hormones and immune function. Cellular Immunology, 252(1-2), 16-26.
https://doi.org/10.1016/j.cellimm.2007.09.006
 
McNeil, J. J., Wolfe, R., Woods, R. L., Tonkin, A. M., Donnan, G. A., Nelson, M. R., Reid, C. M., Lockery, J. E., Kirpach, B., Storey, E., Shah, R. C., Williamson, J. D., Margolis, K. L., Ernst, M. E., Abhayaratna, W. P., Stocks, N, Fitzgerald, S. M., Orchard, S. G., Trevaks, R. E., Beilin, L. J., Johnston, C. I., Ryan, J., Radziszewska, B,, Jelinek, M., Malik, M., Eaton, C. B., Brauer, D., Cloud, G., Wood, E. M., Mahady, S. E., Satterfield,S., Grimm, R., & Murray, A. M. (2018). Effect of Aspirin on Cardiovascular Events and Bleeding in the Healthy Elderly. The New England Journal of Medicine. 379(16), 1509-1518.
https://doi.org/10.1056/NEJMoa1805819
 
Niu, Z. Y., Liu, F. Z., Yan, Q. L., & Li, W. C. (2009). Effects of different levels of vitamin E on growth performance and immune responses of broilers under heat stress. Poultry science, 88(10), 2101-2107.
https://doi.org/10.3382/ps.2009-00220
 
Oladele, S. B., Ayo, J. O., Ogundipe, S. O., & Esievo, K. A. N. (2003). Seasonal and species variations in erythrocytes osmotic fragility of indigenous poultry species in Zaria, Northern Guinea savannah zone of Nigeria. Bulletin of Animal Health and Production in Africa, 51(4), 204-214.
 
Oladele, S. B., Ogundipc, S., Ayo, J. O., & Esievo, K. A. N. (2001). Effects of season and sex on packed cell volume, haemoglobin and total proteins of indigenous pigeons in Zaria, Northern Nigeria. Veterinarski Arhiv, 71(5), 277-286.
 
Oyewale, J. O. (1991). Osmotic fragility of erythrocyte of Guinea fowl at 21 and 156 weeks of age. Veterinary Achieves, 61(1), 49-56.
 
Quinteiro-Filho, W. M., Gomes, A. V. S., Pinheiro, M. L., Ribeiro, A., Ferraz-de-Paula, V., Astolfi-Ferreira, C. S., Ferreira, A. J. P., & Palermo-Neto, J. (2012). Heat stress impairs performance and induces intestinal inflammation in broiler chickens infected with Salmonella Enteritidis. Avian Pathology, 41(5), 421-427.
https://doi.org/10.1080/03079457.2012.709315
 
Quinteiro-Filho, W. M., Ribeiro, A., Ferraz-de-Paula, V., Pinheiro, M. L., Sakai, M., Sá, L. R. M. D., Ferreira, A. J. P., & Palermo-Neto, J. (2010). Heat stress impairs performance parameters, induces intestinal injury, and decreases macrophage activity in broiler chickens. Poultry Science, 89(9), 1905-1914.
https://doi.org/10.3382/ps.2010-00812
 
SAS (2008). Statistical Analysis System, SAS Institute Inc. Cary, NC., USA.
 
Seshasai, S. R., Wijesuriya, S., Sivakumaran, R., Nethercott, S., Erqou, S., Sattar, N.and Ray, K. K. (2012). Effect of aspirin on vascular and nonvascular outcomes: meta-analysis of randomized controlled trials. Archives of Internal Medicine,172(3), 209-216
https://doi.org/10.1001/archinternmed.2011.628
 
Tauseef, M., Shahid, M., Sharma, K. K., & Fahim, M. (2008). Antioxidative action of aspirin on endothelial function in hypercholesterolaemic rats. Basic and Clinical Pharmacology and Toxicology, 103(4), 314-321.
https://doi.org/10.1111/j.1742-7843.2008.00277.x