JOURNAL OF ANIMAL SCIENCE AND VETERINARY MEDICINE
Integrity Research Journals

ISSN: 2536-7099
Model: Open Access/Peer Reviewed
DOI: 10.31248/JASVM
Start Year: 2016
Email: jasvm@integrityresjournals.org


Rotavirus in pigs: Review on structure, epidemiology, risk factors and zoonotic potential

https://doi.org/10.31248/JASVM2025.558   |   Article Number: 4E2252896   |   Vol.10 (3) - June 2025

Received Date: 04 April 2025   |   Accepted Date: 16 June 2025  |   Published Date: 30 June 2025

Authors:  Evalyne Chepkirui Bett* and Wyckliff Ngetich

Keywords: structure, Epidemiology, risk factors., Rotavirus.

Pig production is an important livestock subsector contributing immensely to food security and household income generation, especially in developing countries. Rotavirus infections, which are the leading cause of dehydrating diarrhoea in piglets, hinder their productivity. To date, there are ten Rotavirus groups (A-J) detected and distributed in both humans and animals, with some strains demonstrating zoonotic potential. Some of the risk factors of Rotavirus infection in pig farms include: age of pigs, herd size, presence of other animals, farm management practices and overall sanitation. Evolution of these viruses, especially through reassortment events, has led to the development of novel genotypes with implications for both animal and human health. Rotaviruses are genetically diverse and persist in the environment, and therefore, frequent surveillance, especially molecular characterisation, is of vital importance in the mitigation of these viruses. This review highlights the structure of Rotaviruses, epidemiologic surveillance efforts, identified risk factors, and the potential for interspecies transmission. It also aims to identify research gaps, particularly regarding antigenic variation of circulating strains in pigs within developing countries. Improved surveillance and control measures are essential for enhancing pig health and maximising the economic benefits of pig production.

Alaoui, A. S., Melloul, M., El Alaoui, M. A., Boulahyaoui, H., Loutfi, C., Touil, N., & El Fahime, E. (2020). Evidence for zoonotic transmission of species A rotavirus from goat and cattle in nomadic herds in Morocco, 2012-2014. Virus Genes, 56, 582-593.
https://doi.org/10.1007/s11262-020-01778-w
 
Amimo, J. O., Junga, J. O., Ogara, W. O., Vlasova, A. N., Njahira, M. N., Maina, S., Okoth, E.A., Bishop, R. P., Saif, L. J., & Djikeng, A. (2015). Detection and genetic characterisation of porcine group A rotaviruses in asymptomatic pigs in smallholder farms in East Africa: predominance of P [8] genotype resembling human strains. Veterinary microbiology, 175(2-4), 195-210.
https://doi.org/10.1016/j.vetmic.2014.11.027
 
Amimo, J. O., Otieno, T. F., Okoth, E., Onono, J. O., & Bett, B. (2017). Risk factors for rotavirus infection in pigs in Busia and Teso subcounties, Western Kenya. Tropical animal health and production, 49, 105-112.
https://doi.org/10.1007/s11250-016-1164-9
 
Amimo, J. O., Raev, S. A., Chepngeno, J., Mainga, A. O., Guo, Y., Saif, L., & Vlasova, A. N. (2021). Rotavirus interactions with host intestinal epithelial cells. Frontiers in immunology, 12, 793841.
https://doi.org/10.3389/fimmu.2021.793841
 
Amimo, J. O., Vlasova, A. N., & Saif, L. J. (2013). Detection and genetic diversity of porcine group A rotaviruses in historic (2004) and recent (2011 and 2012) swine faecal samples in Ohio: predominance of the G9P [13] genotype in nursing piglets. Journal of Clinical Microbiology, 51(4), 1142-1151.
https://doi.org/10.1128/JCM.03193-12
 
Arnold, M. M., & Patton, J. T. (2011). Diversity of interferon antagonist activities mediated by NSP1 proteins of different rotavirus strains. Journal of Virology, 85(5), 1970-1979.
https://doi.org/10.1128/JVI.01801-10
 
Bányai, K., Kemenesi, G., Budinski, I., Földes, F., Zana, B., Marton, S., Varga-Kugler, R., Oldal, M., Kurucz, K., & Jakab, F. (2017). Candidate new rotavirus species in Schreiber's bats, Serbia. Infection, Genetics and Evolution, 48, 19-26.
https://doi.org/10.1016/j.meegid.2016.12.002
 
Burrough, E. (2021). Rotaviral Enteritis in Pigs. MSD Veterinary Manual.
 
Chang, K., Kim, Y., Saif, L. J. (2012) Rotavirus and reovirus. Diseases of Swine, 10, 621-634.
 
Chang-Graham, A. L., Perry, J. L., Engevik, M. A., Engevik, K. A., Scribano, F. J., Gebert, J. T., Danhof, H. A., & Hyser, J. M. (2020). Rotavirus induces intercellular calcium waves through ADP signaling. Science, 370(6519), eabc3621.
https://doi.org/10.1126/science.abc3621
 
Chasey, D., Bridger, J. C., & McCrae, M. A. (1986). A new type of atypical rotavirus in pigs. Archives of Virology, 89, 235-243.
https://doi.org/10.1007/BF01309892
 
Chatzopoulos, D. C., Athanasiou, L. V., Spyrou, V., Fthenakis, G. C., & Billinis, C. (2013). Rotavirus infections in domestic animals. Journal of the Hellenic Veterinary Medical Society, 64(2), 145-160.
https://doi.org/10.12681/jhvms.15489
 
Chepngeno, J., Diaz, A., Paim, F. C., Saif, L. J., & Vlasova, A. N. (2019). Rotavirus C: Prevalence in suckling piglets and development of virus-like particles to assess the influence of maternal immunity on the disease development. Veterinary Research, 50, 1-12.
https://doi.org/10.1186/s13567-019-0705-4
 
Chepngeno, J., Takanashi, S., Diaz, A., Michael, H., Paim, F. C., Rahe, M. C., Hayez, J.R., Baker, C. & Vlasova, A. N. (2020). Comparative sequence analysis of historic and current porcine rotavirus c strains and their pathogenesis in 3-day-old and 3-week-old piglets. Frontiers in Microbiology, 11, 780.
https://doi.org/10.3389/fmicb.2020.00780
 
Collins, P. J., Martella, V., Sleator, R. D., Fanning, S., & O'shea, H. (2010). Detection and characterisation of group A rotavirus in asymptomatic piglets in southern Ireland. Archives of Virology, 155, 1247-1259.
https://doi.org/10.1007/s00705-010-0713-1
 
Cook, N., Bridger, J., Kendall, K., Gomara, M. I., El-Attar, L., & Gray, J. (2004). The zoonotic potential of rotavirus. Journal of Infection, 48(4), 289-302.
https://doi.org/10.1016/j.jinf.2004.01.018
 
Costa, F. B., Flores, P. S., Amorim, A. R., Mendes, G. D. S., & Santos, N. (2020). Porcine rotavirus C strains carrying human‐like NSP4 and NSP5. Zoonoses and Public Health, 67(8), 849-861.
https://doi.org/10.1111/zph.12713
 
Costantini, V. P., Azevedo, A. C., Li, X., Williams, M. C., Michel Jr, F. C., & Saif, L. J. (2007). Effects of different animal waste treatment technologies on detection and viability of porcine enteric viruses. Applied and Environmental Microbiology, 73(16), 5284-5291.
https://doi.org/10.1128/AEM.00553-07
 
Crawford, S. E., Ramani, S., Tate, J. E., Parashar, U. D., Svensson, L., Hagbom, M., Franco, M. A., & Estes, M. K. (2017). Rotavirus infection. Nature Reviews Disease Primers, 3(1), 1-16.
https://doi.org/10.1038/nrdp.2017.83
 
Cui, T., Theuns, S., Xie, J., & Nauwynck, H. J. (2019). Porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface. Veterinary Research, 50, 1-13.
https://doi.org/10.1186/s13567-019-0728-x
 
Delia, T. A., Dzikwi-Emennaa, A. A., Kwaga, J. K. P., Kia, G. S. N., Olufemi, O. T., Otolorin, G. R., & Adanu, A. W. (2019). Prevalence of porcine rotavirus antigen and associated risk factors in pig-raising communities and institutional piggeries in Zaria, Kaduna state, Nigeria. Folia Veterinaria, 63(1), 17-23.
https://doi.org/10.2478/fv-2019-0003
 
Doerksen, T., Christensen, T., Lu, A., Noll, L., Bai, J., Henningson, J., & Palinski, R. (2022). Assessment of porcine Rotavirus-associated virome variations in pigs with enteric disease. Veterinary Microbiology, 270, 109447.
https://doi.org/10.1016/j.vetmic.2022.109447
 
Doro, R., Farkas, S. L., Martella, V., & Banyai, K. (2015). Zoonotic transmission of rotavirus: surveillance and control. Expert review of anti-infective therapy, 13(11), 1337-1350.
https://doi.org/10.1586/14787210.2015.1089171
 
Estes, M. K., & Greenberg, H. B. (2013). Rotaviruses. In: Fields, B. N., Knipe, D. M., & Howley, P. M. (eds.). Fields virology (pp. 1347-1401), 6th edition. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins.
 
Estes, M. K., Kang, G., Zeng, C. Q. Y., Crawford, S. E., & Ciarlet, M. (2001, May). Pathogenesis of rotavirus gastroenteritis. In Gastroenteritis Viruses: Novartis Foundation Symposium, 238 (Vol. 238, pp. 82-100). Chichester, UK: John Wiley & Sons, Ltd.
https://doi.org/10.1002/0470846534.ch6
 
Ferrari, E., Salogni, C., Martella, V., Alborali, G. L., Scaburri, A., & Boniotti, M. B. (2022). Assessing the epidemiology of rotavirus A, B, C and H in diarrheic pigs of different ages in northern Italy. Pathogens, 11(4), 467.
https://doi.org/10.3390/pathogens11040467
 
Flores, P. S., Costa, F. B., Amorim, A. R., Mendes, G. S., Rojas, M., & Santos, N. (2021). Rotavirus A, C, and H in Brazilian pigs: Potential for zoonotic transmission of RVA. Journal of Veterinary Diagnostic Investigation, 33(1), 129-135.
https://doi.org/10.1177/1040638720967673
 
Fongaro, G., Padilha, J., Schissi, C. D., Nascimento, M. A., Bampi, G. B., Viancelli, A., & Barardi, C. R. M. (2015). Human and animal enteric virus in groundwater from deep wells, and recreational and network water. Environmental Science and Pollution Research, 22, 20060-20066.
https://doi.org/10.1007/s11356-015-5196-x
 
Food and Agriculture Organisation of the United Nations (FAO) (2021). Meat market review. Retrieved from http://www.fao.org/3/cb3700en/cb3700en.pdf.
 
Guo, Y., Candelero-Rueda, R. A., Saif, L. J., & Vlasova, A. N. (2021). Infection of porcine small intestinal enteroids with human and pig rotavirus A strains reveals contrasting roles for histo-blood group antigens and terminal sialic acids. PLoS Pathogens, 17(1), e1009237.
https://doi.org/10.1371/journal.ppat.1009237
 
Homwong, N., Diaz, A., Rossow, S., Ciarlet, M., & Marthaler, D. (2016). Three-level mixed-effects logistic regression analysis reveals complex epidemiology of swine rotaviruses in diagnostic samples from North America. PLoS One, 11(5), e0154734.
https://doi.org/10.1371/journal.pone.0154734
 
Holloway, G., & Coulson, B. S. (2013). Innate cellular responses to rotavirus infection. Journal of General Virology, 94(6), 1151-1160.
https://doi.org/10.1099/vir.0.051276-0
 
Jiang, X., Liu, Y., & Tan, M. (2017). Histo-blood group antigens as receptors for rotavirus, new understanding on rotavirus epidemiology and vaccine strategy: Rotavirus host receptor and vaccine strategy. Emerging Microbes & Infections, 6(1), 1-8.
https://doi.org/10.1038/emi.2017.30
 
Kattoor, J. J., Saurabh, S., Malik, Y. S., Sircar, S., Dhama, K., Ghosh, S., Banyai, K., Kobayashi, N. & Singh, R. K. (2017). Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene. Veterinary Quarterly, 37(1), 252-261.
https://doi.org/10.1080/01652176.2017.1346849
 
Kumar, D., Shepherd, F. K., Springer, N. L., Mwangi, W., & Marthaler, D. G. (2022). Rotavirus infection in swine: genotypic diversity, immune responses, and role of gut microbiome in rotavirus immunity. Pathogens, 11(10), 1078.
https://doi.org/10.3390/pathogens11101078
 
Lee, C. (2020). Controversial effects of vitamin D and related genes on viral infections, pathogenesis, and treatment outcomes. Nutrients, 12(4), 962.
https://doi.org/10.3390/nu12040962
 
Li, Q., Wang, Z., Jiang, J., He, B., He, S., Tu, C., Guo, Y. & Gong, W. (2024). Outbreak of piglet diarrhoea associated with a new reassortant porcine rotavirus B. Veterinary Microbiology, 288, 109947.
https://doi.org/10.1016/j.vetmic.2023.109947
 
Malik, Y. S., Bhat, S., Dar, P. S., Sircar, S., Dhama, K., & Singh, R. K. (2020). Evolving rotaviruses, interspecies transmission and zoonoses. The Open Virology Journal, 14, 1-6.
https://doi.org/10.2174/1874357902014010001
 
Mao, X., Gu, C., Ren, M., Chen, D., Yu, B., He, J., Yu, J., Zheng, P., Luo, J., Luo, Y., Wang, J., Tian, G. & Yang, Q. (2018). L-isoleucine administration alleviates rotavirus infection and immune response in the weaned piglet model. Frontiers in Immunology, 9, 1654.
https://doi.org/10.3389/fimmu.2018.01654
 
Marthaler, D., Homwong, N., Rossow, K., Culhane, M., Goyal, S., Collins, J., Matthijnssens, J. & Ciarlet, M. (2014a). Rapid detection and high occurrence of porcine rotavirus A, B, and C by RT-qPCR in diagnostic samples. Journal of Virological Methods, 209, 30-34.
https://doi.org/10.1016/j.jviromet.2014.08.018
 
Marthaler, D., Rossow, K., Culhane, M., Collins, J., Goyal, S., Ciarlet, M., & Matthijnssens, J. (2013). Identification, phylogenetic analysis and classification of porcine group C rotavirus VP7 sequences from the United States and Canada. Virology, 446(1-2), 189-198.
https://doi.org/10.1016/j.virol.2013.08.001
 
Marthaler, D., Rossow, K., Culhane, M., Goyal, S., Collins, J., Matthijnssens, J., Nelson, M., & Ciarlet, M. (2014b). Widespread rotavirus H in commercially raised pigs, United States. Emerging Infectious Diseases, 20(7), 1203.
https://doi.org/10.3201/eid2007.140034
 
Matthijnssens, J., Attoui, H., Bányai, K., Brussaard, CP, Danthi, P., Del Vas, M., Dermody, T.S., Duncan, R., Fang, Q., Johne, R., & Wei, T. (2022). ICTV virus taxonomy profile: Sedoreoviridae 2022. Journal of General Virology, 103 (10), 001782.
https://doi.org/10.1099/jgv.0.001782
 
Miyabe, F. M., Dall Agnol, A. M., Leme, R. A., Oliveira, T. E. S., Headley, S. A., Fernandes, T., Goncalves, A., Alfieri, A.F., & Alfieri, A. A. (2020). Porcine rotavirus B as primary causative agent of diarrhoea outbreaks in newborn piglets. Scientific Reports, 10(1), 22002.
https://doi.org/10.1038/s41598-020-78797-y
 
Monteagudo, L. V., Benito, A. A., Lázaro-Gaspar, S., Arnal, J. L., Martin-Jurado, D., Menjon, R., & Quílez, J. (2022). Occurrence of rotavirus A genotypes and other enteric pathogens in diarrheic suckling piglets from Spanish swine farms. Animals, 12(3), 251.
https://doi.org/10.3390/ani12030251
 
Murao, L. A. E., Bacus, M. G., Junsay, N. X. T., Albarillo, D. L. D., Otero, M. C. B., Buenaventura, S. G. C., Ligue, K.D.B. & Alviola, P. A. (2019). Spatiotemporal dynamics and risk factors of rotavirus A circulation in backyard pig farms in a Philippine setting. Tropical Animal Health and Production, 51, 929-937.
https://doi.org/10.1007/s11250-018-1776-3
 
Nguyen, T. V., Yuan, L., Azevedo, M. S., Jeong, K. I., González, A. M., & Saif, L. J. (2007). Transfer of maternal cytokines to suckling piglets: in vivo and in vitro models with implications for immunomodulation of neonatal immunity. Veterinary Immunology and Immunopathology, 117(3-4), 236-248.
https://doi.org/10.1016/j.vetimm.2007.02.013
 
Nyaga, M. M., Jere, K. C., Esona, M. D., Seheri, M. L., Stucker, K. M., Halpin, R. A., Akopov, A., & Mphahlele, M. J. (2015). Whole genome detection of rotavirus mixed infections in human, porcine and bovine samples co-infected with various rotavirus strains collected from sub-Saharan Africa. Infection, Genetics and Evolution, 31, 321-334.
https://doi.org/10.1016/j.meegid.2015.02.011
 
Papp, H., László, B., Jakab, F., Ganesh, B., De Grazia, S., Matthijnssens, J., Ciarlet, M., Martella, V & Bányai, K. (2013). Review of group A rotavirus strains reported in swine and cattle. Veterinary microbiology, 165(3-4), 190-199.
https://doi.org/10.1016/j.vetmic.2013.03.020
 
Park, J.G., Alfajaro, M.M., Cho, E. H., Kim, J. Y., Soliman, M., Baek, Y.B., Lee, J. H., & Kang, M. I. (2019). Development of a live attenuated trivalent porcine rotavirus A vaccine against disease caused by recent strains most prevalent in South Korea. Veterinary Research, 50, 1-16.
https://doi.org/10.1186/s13567-018-0619-6
 
Patel, M. M., Pitzer, V. E., Alonso, W. J., Vera, D., Lopman, B., Tate, J., Vibound, C., & Parashar, U. D. (2013). Global seasonality of rotavirus disease. The Pediatric Infectious Disease Journal, 32(4), e134-e147.
https://doi.org/10.1097/INF.0b013e31827d3b68
 
Qiao, M., Li, M., Li, Y., Wang, Z., Hu, Z., Qing, J., Huang, J., Jiang, J., Jiang, Y., Zhang, J., Gao, C., Yang, C., Li, X., & Zhou, B. (2024). Recent molecular characterization of porcine rotaviruses detected in China and their phylogenetic relationships with human Rotaviruses. Viruses, 16(3), 453.
https://doi.org/10.3390/v16030453
 
Saif, L., & Vlasova, A. (2022) Rotaviral diarrhoea in pigs. Pork Information Gateway. Retrieved from https://porkgateway.org/resource/rotaviral-diarrhea-in-pigs/
 
Santiana, M., Ghosh, S., Ho, B. A., Rajasekaran, V., Du, W. L., Mutsafi, Y., Jesus-Diaz, D. D., & Altan-Bonnet, N. (2018). Vesicle-cloaked virus clusters are optimal units for inter-organismal viral transmission. Cell Host & Microbe, 24(2), 208-220.
https://doi.org/10.1016/j.chom.2018.07.006
 
Shepherd, F. K., Freeman, M. J., Culhane, M. R., Marthaler, D. G. (2019). Reoviruses (Rotaviruses and Reoviruses). In: Zimmerman, J. J., Karriker, L. A., Ramirez, A., Schwartz, K.J., Stevenson, G. W., & Zhang, J. (eds.). Diseases of swine (pp. 715-727), Wiley Online Library.
https://doi.org/10.1002/9781119350927.ch43
 
Shepherd, F. K., Herrera-Ibata, D. M., Porter, E., Homwong, N., Hesse, R., Bai, J., & Marthaler, D. G. (2018). Whole genome classification and phylogenetic analyses of rotavirus B strains from the United States. Pathogens, 7(2), 44.
https://doi.org/10.3390/pathogens7020044
 
Shepherd, F. K., Murtaugh, M. P., Chen, F., Culhane, M. R., & Marthaler, D. G. (2017). Longitudinal surveillance of porcine rotavirus B strains from the United States and Canada and in silico identification of antigenically important sites. Pathogens, 6(4), 64.
https://doi.org/10.3390/pathogens6040064
 
Suzuki, T., & Inoue, D. (2018). Full genome-based genotyping system for rotavirus H and detection of potential gene recombination in nonstructural protein 3 between porcine rotavirus H and rotavirus C. Journal of General Virology, 99(12), 1582-1589.
https://doi.org/10.1099/jgv.0.001162
 
Theuns, S., Desmarets, L. M., Heylen, E., Zeller, M., Dedeurwaerder, A., Roukaerts, I. D., & Nauwynck, H. J. (2014). Porcine group A rotaviruses with heterogeneous VP7 and VP4 genotype combinations can be found together with enteric bacteria on Belgian swine farms. Veterinary Microbiology, 172(1-2), 23-34.
https://doi.org/10.1016/j.vetmic.2014.04.002
 
Theuns, S., Vyt, P., Desmarets, L. M., Roukaerts, I. D., Heylen, E., Zeller, M., Matthijnssens, J. & Nauwynck, H. J. (2016). Presence and characterization of pig group A and C rotaviruses in faeces of Belgian diarrheic suckling piglets. Virus Research, 213, 172-183.
https://doi.org/10.1016/j.virusres.2015.12.004
 
Tian, G., Liang, X., Chen, D., Mao, X., Yu, J., Zheng, P., Huang, Z., & Yu, B. (2016). Vitamin D3 supplementation alleviates rotavirus infection in pigs and IPEC-J2 cells via regulating the autophagy signalling pathway. The Journal of Steroid Biochemistry and Molecular Biology, 163, 157-163.
https://doi.org/10.1016/j.jsbmb.2016.05.004
 
Tuanthap, S., Phupolphan, C., Luengyosluechakul, S., Duang-In, A., Theamboonlers, A., Wattanaphansak, S., Vongpunsawad, S., Amonsin, A. & Poovorawan, Y. (2018). Porcine rotavirus C in pigs with gastroenteritis on Thai swine farms, 2011-2016. PeerJ Microbiology, 6, e4724.
https://doi.org/10.7717/peerj.4724
 
VinodhKumar, O. R., Sircar, S., Pruthvishree, B. S., Nirupama, K. R., Singh, B. R., Sinha, D. K., Rupner, R., & Malik, Y. S. (2020). Cross-sectional study on rotavirus A (RVA) infection and assessment of risk factors in pre-and post-weaning piglets in India. Tropical Animal Health and Production, 52, 445-452.
https://doi.org/10.1007/s11250-019-01999-8
 
Vlasova, A. N., Amimo, J. O., & Saif, L. J. (2017). Porcine rotaviruses: epidemiology, immune responses and control strategies. Viruses, 9(3), 48.
https://doi.org/10.3390/v9030048
 
Wu, F. T., Bányai, K., Jiang, B., Liu, L. T. C., Marton, S., Huang, Y. C., Huang, L.M., Liao, M.H. & Hsiung, C. A. (2017). Novel G9 rotavirus strains co-circulate in children and pigs, Taiwan. Scientific Reports, 7(1), 40731.
https://doi.org/10.1038/srep40731
 
Wu, F. T., Liu, L. T. C., Jiang, B., Kuo, T. Y., Wu, C. Y., & Liao, M. H. (2022). Prevalence and diversity of rotavirus A in pigs: Evidence for a possible reservoir in human infection. Infection, Genetics and Evolution, 98, 105198.
https://doi.org/10.1016/j.meegid.2021.105198