ISSN: 2536-7072
Model: Open Access/Peer Reviewed
DOI: 10.31248/JASP
Start Year: 2016
Email: jasp@integrityresjournals.org
https://doi.org/10.31248/JASP2026.576 | Article Number: E9D64C513 | Vol.11 (1) - April 2026
Received Date: 25 March 2026 | Accepted Date: 24 April 2026 | Published Date: 30 April 2026
Authors: Uba, D. , Fakae, L. B. , Johnson, N. C. and Diri, M.*
Keywords: oxidative stress, vitamin C., Ginger, Antioxidants, broiler chicken, malondialdehyde
The antioxidant and pro-oxidant responses of broiler chickens to dietary ginger and vitamin C were investigated. Antioxidants studied included glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD), while malondialdehyde (MDA) served as the pro-oxidant indicator. One hundred and fifty (150) unsexed Agrited day-old chicks were used. On arrival, chicks were brooded for 4 weeks before being randomly assigned to five dietary treatment groups. Each group comprised 30 birds with three (3) replicates of ten (10) birds each. Ginger was fixed at 10 g/kg while vitamin C was graded as follows: Treatment 1 (T1), the negative control, contained 0 g/kg ginger + 0 mg/kg vitamin C; T2: 10 g/kg ginger + 100 mg/kg vitamin C; T3: 10 g/kg ginger + 200 mg/kg vitamin C; T4: 10 g/kg ginger + 300 mg/kg vitamin C; and T5: 10 g/kg ginger + 400 mg/kg vitamin C. After 4 weeks on their respective experimental diets, 9 birds per group (3 per replicate) were sacrificed for blood collection. GSH, GPx, CAT, and SOD levels in T2–T5 were significantly (p<0.05) higher than in T1, with T3 recording the highest values. MDA levels were significantly (p<0.05) highest in T1 and T5, and lowest in T3. It was concluded that dietary ginger and vitamin C improve antioxidant status and reduce oxidative stress in broiler chickens, particularly at the combination of 10 g/kg ginger and 200 mg/kg vitamin C.
| Abubakar, J. O., Uchechi, N. C., Olayinka Abosede, O., & Oladimeji Samuel, T. (2023). Role of oral phytogenic supplementation to protect cardiac, hepatic, nephrotic, and splenic oxidative stress in broiler chickens. Translational Animal Science, 7(1), txad106. https://doi.org/10.1093/tas/txad106 |
||||
| Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105, 121-126. https://doi.org/10.1016/S0076-6879(84)05016-3 |
||||
| Agergaard, N., & Jensen, P. T. (1982). Microassay for glutathione in blood. Acta Veterinaria Scandinavica, 23(4), 520-528. https://doi.org/10.1186/BF03546770 |
||||
| Al-Khalaifah, H., Al-Nasser, A., Al-Surrayai, T., Sultan, H., Al-Attal, D., Al-Kandari, R., Al-Saleem, H., Al-Holi, A., & Dashti, F. (2022). Effect of ginger powder on production performance, antioxidant status, hematological parameters, digestibility, and plasma cholesterol content in broiler chickens. Animals, 12(7), 901. https://doi.org/10.3390/ani12070901 |
||||
| Dosu, G., Obanla, T. O., Zhang, S., Sang, S., Adetunji, A. O., Fahrenholz, A. C., Ferket, P. R., Nagabhushanam, K., & Fasina, Y. O. (2023). Supplementation of ginger root extract into broiler chicken diet: Effects on growth performance and immunocompetence. Poultry Science, 102(10), 102897. https://doi.org/10.1016/j.psj.2023.102897 |
||||
| Fatima, H. (2026). Dietary vitamin C improves growth performance and stress resistance in broiler chickens. Trends in Animal and Plant Sciences, 7, 31-37. | ||||
| Giannenas, I., Bonos, E., Filliousis, G., Stylianaki, I., Kumar, P., Lazari, D., Christaki, E., & Florou-Paneri, P. (2019). Effect of a polyherbal or an arsenic-containing feed additive on growth performance of broiler chickens, intestinal microbiota, intestinal morphology, and lipid oxidation of breast and thigh meat. Journal of Applied Poultry Research, 28(1), 164-175. https://doi.org/10.3382/japr/pfy059 |
||||
| Gouda, A., Amer, S. A., Gabr, S., & Tolba, S. A. (2020). Effect of dietary supplemental ascorbic acid and folic acid on the growth performance, redox status, and immune status of broiler chickens under heat stress. Tropical Animal Health and Production, 52(6), 2987-2996. https://doi.org/10.1007/s11250-020-02316-4 |
||||
| Kairalla, M. A., Aburas, A. A., & Alshelmani, M. I. (2022). Effect of diet supplemented with graded levels of ginger (Zingiber officinale) powder on growth performance, hematological parameters, and serum lipids of broiler chickens. Archives of Razi Institute, 77(6), 2077-2083. https://doi.org/10.22092/ARI.2022.359958.2524 | ||||
| Misra, H. P., & Fridovich, I. (1972). The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological Chemistry, 247(10), 3170-3175. https://doi.org/10.1016/S0021-9258(19)45228-9 |
||||
| Putri, R. U., Widyarini, S., Wasita, B., & Yuliani, R. (2024). A critical review of ginger's (Zingiber officinale) antioxidant, anti-inflammatory, and immunomodulatory activities. Frontiers in Veterinary Science, 11, 1387259. https://doi.org/10.3389/fnut.2024.1364836 |
||||
| Rafeeq, M., Bilal, R. M., Batool, F., Yameen, K., Farag, M. R., Madkour, M., Elnesr, S. S., El-Shall, N. A., Dhama, K., & Alagawany, M. (2023). Application of herbs and their derivatives in broiler chickens: A review. World's Poultry Science Journal, 79(1), 95-117. https://doi.org/10.1080/00439339.2022.2151395 |
||||
| SAS Institute Inc. (2012). SAS/STAT® 9.4 User's Guide. SAS Institute Inc. | ||||
| Shakeri, M., Oskoueian, E., Le, H. H., & Shakeri, M. (2020). Strategies to combat heat stress in broiler chickens: Unveiling the roles of selenium, vitamin E and vitamin C. Veterinary Sciences, 7(2), 71. https://doi.org/10.3390/vetsci7020071 |
||||
| Van Hieu, T., Guntoro, B., Qui, N. H., Quyen, N. T. K., & Al Hafiz, F. A. (2022). The application of ascorbic acid as a therapeutic feed additive to boost immunity and antioxidant activity of poultry in heat stress environment. Veterinary World, 15(3), 685-693. https://doi.org/10.14202/vetworld.2022.685-693 |
||||
| Varshney, R., & Kale, R. K. (1990). Effects of calmodulin antagonists on radiation-induced lipid peroxidation in microsomes. International Journal of Radiation Biology, 58(5), 733-743. https://doi.org/10.1080/09553009014552121 |
||||