JOURNAL OF AGRICULTURAL SCIENCE AND PRACTICE
Integrity Research Journals
ISSN: 2536-7072
Model: Open Access/Peer Reviewed
DOI: 10.31248/JASP
Start Year: 2016
Email: jasp@integrityresjournals.org
Free radical scavenging and metal ion chelating properties of differently pigmented rice varieties
https://doi.org/10.31248/JASP2020.216 | Article Number: 514CA0545 | Vol.5 (3) - June 2020
Received Date: 27 May 2020 | Accepted Date: 19 June 2020 | Published Date: 30 June 2020
Authors: Sandy Pradipta , Mohammad Ubaidillah and Tri Agus Siswoyo*
Keywords: phytochemical, Antioxidants, Anthocyanins, carotenoids, flavonoid
Pigmented rice (Oryza sativa L.) becomes interestingly important in agroindustry because it contains high phytochemical compounds that can be antioxidants and metal ion chelating. This study attempted to relate the content of phenols, flavonoids, anthocyanin, and bioactive phytochemicals activity from differently pigmented rice (red and black) varieties. The bioactive phytochemical activities were evaluated by measuring free radical scavenging, hydroxyl scavenging, and metal ion chelating activity. The result showed that the highest phenolic, flavonoids, and anthocyanin content found in black rice, followed by red rice and non-pigmented rice. The anthocyanin in black rice was significantly higher (p £ 0.05) than in red and non-pigmented rice. The chlorophyll and carotenoids contents in all samples varied from 0.03 to 1.42 µg g-1 and 0.26 to 0.53 µg g-1, respectively. The antioxidant and metal ion chelating activities in black and red rice were significantly higher than non-pigmented rice. The correlation analysis showed that the phenols, flavonoids, and phytochemical pigments have a positive correlation (p £ 0.05) with antioxidant and metal chelating activity. In this study, black and red rice varieties showed the potential natural resources for the important effect of nutraceutical food products and pharmaceuticals.
| Abdel-Aal, E. S. M. & Hucl, P. (1997). A rapid method for quantifying total anthocyanins in blue aleurone and purple pericarp wheats. Cereal Chemistry, 76(3), 350-354, Crossref |
||||
| Adusei, S., Otchere, J. K., Oteng, P., Mensah, R. Q., & Tei-Mensah, E. (2019). Phytochemical analysis, antioxidant and metal chelating capacity of Tetrapleura tetraptera. Heliyon, 5(11), 1-5. Crossref |
||||
| Ashraf, H., Murtaza, I., Nazir, N., Wani, A. B., Naqash, S., & Husaini, A.M. (2017). Nutritional profiling of pigmented and scented rice genotypes of Kashmir Himalayas. Journal of Pharmacognosy and Phytochemistry, 6(6), 910-916. | ||||
| Ashraf, M. A. (2020). Phytochemicals as potential anticancer drugs: time to ponder nature's bounty. Biomed Research International, Volume 2020, Article ID 8602879, 7 pages. Crossref |
||||
| Baek, J. A., Chung, N. J., Choi, K. C., Hwang, J. M., & Lee J. C. (2015). Hull extracts from pigmented rice exert antioxidant effects associated with total flavonoid contents and induce apoptosis in human cancer cells. Food Science and Biotechnology, 24(1), 241-247. Crossref |
||||
| Crisponi, G., Dean, A., Di Marco, V., Lachowicz, J. I., Nurchi, V. M., Remelli, M., & Tapparo, A. (2013). Different approaches to the study of chelating agents for iron and aluminium overload pathologies. Analytical and Bioanalytical Chemistry, 405(2-3), 585-601. Crossref |
||||
| Deng, G., Xu R., Zhang, Y., Li, D., Gan, R., & Li, H. (2013). Phenolic compounds and bioactivities of pigmented rice. Critical Reviews in Food Science and Nutrition, 533(3), 296-306. Crossref |
||||
| Djeridane, A., Yousfi, M., Nadjemi, B., Boutassouna, D., Stocker, P., & Vidal, N. (2006). Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 97(4), 654-660. Crossref |
||||
| Falade, K. O., & Christopher, A. S. (2015). Physical, functional, pasting and thermal properties of flours and starches of six Nigerian rice cultivars. Food Hydrocolloids, 44, 478-490. Crossref |
||||
| Fiedor, J., & Burda, K. (2014). Potential role of carotenoids as antioxidants in human health and disease. Nutrients, 6(2), 466-88. Crossref |
||||
| Flora, S. J., & Pachauri, V. (2010). Chelation in metal intoxication. International Journal of Environmental Research and Public Health, 7(7), 2745-2788. Crossref |
||||
| Gayathri, G., Nair, B. R., & Babu, V. (2014). Scavenging of free radicals and total phenols of methanol extract of Azima tetracantha Lam. International Journal of Pharmacy and Pharmaceutical Sciences, 6(9), 347-351. | ||||
| Ghasemzadeh, A., Karbalaii, M. T., Jaafar, H. Z. E., & Rahmat, A. (2018). Phytochemical constituents, antioxidant activity, and antiproliferative properties of black, red, and brown rice bran. Chemistry Central Journal, 12(17), 1-13. Crossref |
||||
| Goufo, P., & Trindade, H. (2014). Rice antioxidants: phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, c-oryzanol, and phytic acid. Food Science and Nutrition, 2(2), 75-104. Crossref |
||||
| Halliwell, B., Gutteridge, J. M. C., & Aruoma, O. I. (1987). The deoxyribose method: A simple "test-tube" assay for determination of rate constants for reactions of hydroxyl radicals. Analytical Biochemistry, 165(1), 215-219. Crossref |
||||
| Halliwell, B. (1987). Oxidants and human disease: some new concepts 1. The FASEB Journal, 1(5), 358-364. Crossref |
||||
| Hemamalini, S., Umamaheswari, D. S., Lavanya, D. R., & Umamaheswara, R. D. C. (2018). Exploring the therapeutic potential and nutritional properties of 'Karuppu Kavuni' variety rice of Tamil Nadu. International Journal of Pharma and Bio Science, 9(1), 88-96. Crossref |
||||
| Hamidu. L., Ahmad, A. R., & Najib, A. (2018). Qualitative and quantitative test of total flavonoid buni fruit (Antidesma bunius (L.) spreng) with uv-vis spectrophotometry method. Pharmacognosy Journal, 10(1), 60-63. Crossref |
||||
| Henry, G. A. F. & Grime J. P. (1993). Methods in Comparative Plant Ecology, Chapman & Hall, London. Pp. 148-152. Crossref |
||||
| Heuberger, A. L., Lewis, M. R., Cen, M., Brick, M. A., Leach, J. E., & Ryan, E. P. (2010). Metabolomic and functional genomic analyses reveal varietal differences in bioactive compounds of cooked rice. Plos One, 5(9), 1-10. Crossref |
||||
| Kim, B. G., Kim, J H., Min, S. Y., Shin, K-H., Kim, J. H., Kim, H. Y., & Ryu, S. N. (2007). Anthocyanin content in rice is related to expression levels of anthocyanin biosynthetic genes. Journal of Plant Biology, 50(2), 156-160. Crossref |
||||
| Kraithong, S., Lee, S., & Rawdkuen, S. (2018). Physicochemical and functional properties of Thai organic rice flour. Journal of Cereal Science, 79, 259-266. Crossref |
||||
| Khoo, H. E., Azlan, A., Tang, S. T., & Lim, S. M. (2017). Anthocyanidins and anthocyanins: colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food and Nutrition Research, 61 (1), 1-21. Crossref |
||||
| Limtrakul, P., Yodkeeree, S., Pitchakarn, P., & Punfa, W. (2016). Anti-flammatory effects of proanthocyanidin rich red rice extract via suppression of MAPK, AP-1 and NF- j B pathways in Raw 264.7 macrophages. Nutrition Research and Practice, 10(3), 251-258. Crossref |
||||
| Lipinski, B. (2011). Hydroxyl radical and its scavengers in health and disease. Oxidative medicine and cellular longevity, Volume 2011, Article ID 809696, 9 pages. Crossref |
||||
| Maiani, G. Castón, M. J. P., Catasta, G., Toti, E., Cambrodón, I. G., Bysted, A., Granado-Lorencio, F., Olmedilla-Alonso, B., Knuthsen, P., & Valoti, M. (2009). Carotenoids: Actual knowledge on food sources, intakes, stability and bio availability and their protective role in humans. Molecular Nutrition & Food Research, 53(2), 194-218. Crossref |
||||
| Mbanjo, E. G. N., Kretzchmar, T., Jones, H., Ereful, N., Blanchard, C., Boyd, L. N., & Sreenivasulu, N. (2020). The genetic basis and nutritional benefits of pigmented rice grain. Frontiers in Genetics, 11(29), 1-18. Crossref |
||||
| Melini, V., & Rita, A. (2017). Health-promoting compounds in pigmented thai and wild rice. Foods, 6(9), 1-13. Crossref |
||||
| Mozaffarieh, M., Sacu, S., & Wedrich, A. (2003). The role of the carotenoids, lutein and zeaxanthin, in protecting against age-related macular degeneration: A review based on controversial evidence. Nutrition Journal, 2(1), 1-8. Crossref |
||||
| Mudoi, T., & Das, P. A. (2019). Study on phytochemicals and mineral content of indigenous red rice of Assam, India. International Journal of Current Microbiology and Applied Sciences, 8(4), 1-12. Crossref |
||||
| Muller, L., Frohlich, K., & Bohm, V. (2011). Comparative antioxidant activities of carotenoids measured by ferric reducing antioxidant power ( FRAP), ABTS bleaching assay (TEAC), DPPH assay, and peroxyl radical scavenging assay. Food Chemistry, 129(1), 139-148. Crossref |
||||
| Nam, S. H., Choi, S. P., Kang, M. Y., & Koh, H. J. (2006). Kozukue N., Friedman M. Antioxidative activities of bran extracts from twenty one pigmented rice cultivars. Food Chemistry, 94(4), 613-620. Crossref |
||||
| Oki, T., Masuda, M., Kobayashi, M., Nishiba, Y., Furuta, S., Suda, I., & Sato, T. (2002). Polymeric procyanidins as radical-scavenging components in red-hulled rice. Journal of Agricultural and Food, 50(26), 7524-7529. Crossref |
||||
| Palombini, S. V., Maruyama, S. A., Claus, T., Carbonera, .F, Souza, N. E. D., Visentainer, J. V., Gomes, S. T. M., & Matsushita, M. (2013). Evaluation of antioxidant potential of Brazilian rice cultivars. Food Science and Technology, 33(4), 699-704. Crossref |
||||
| Pechinskii, S.V., & Kuregyan, A. G. (2014). The impact of carotenoids on immunity (Review). Pharmaceutical Chemistry Journal, 47(10), 509-513. Crossref |
||||
| Pereira-Caro, G., Cros, G., Yokot,a T., & Crozier, A. (2013). Phytochemical profiles of black, red, brown, and white rice from the Camargue region of France. Journal of Agricultural and Food Chemistry, 61(33), 7976-7986. Crossref |
||||
| Pavithra, K., & Vadivukkarasi, S. (2015). Evaluation of free radical scavenging activity of various extracts of leaves from Kedrostis foetidissima (Jacq.) Cogn. Food Science and Human Wellness, 4(1), 42-46. Crossref |
||||
| Punvittayagul, C., Sringarm, K., Chaiyasut C., & Wongpoomchai, R. (2014). Mutagenicity and antimutagenicity of hydrophilic and lipophilic extracts of Thai northern purple rice. Asian Pacific Journal of Cancer Prevention, 15(21), 9517-9522. Crossref |
||||
| Rahman, M. M., Lee, K. E., Lee, E. S., Matin, M. N., Lee, D. S.,Yun, J. S., kim, J. B., & Kang, S. G. (2013). The geneticconstitutions of complementary genes Pp and Pbdetermine the purple color variation in pericarps withcyanidin-3-0-glucoside depositions in black Rice. Journal of Plant Biology, 56, 24-31. Crossref |
||||
| Rahman, M. M., Lee, K. E., & Kang, S. G. (2015). Studies on the effects of pericarp pigmentation on grain development and yield of black rice. Indian Journal of Genetics and Plant Breeding, 75(4), 426-433. Crossref |
||||
| Samyor, D., Deka, S. C., & Das, A. B. (2016). Phytochemical and antioxidant profile of pigmented and non-pigmented rice cultivars of Arunachal Pradesh, India. International Journal of Food Properties, 19(5), 1104-1114. Crossref |
||||
| Shen, Y., Jin, L., Xiao, P., Lu, Y., & Bao, J. (2009). Total phenolics, flavonoids, antioxidant capacity in rice grain and their relations to grain color, size and weight. Food Chemistry, 49(1), 106-111. Crossref |
||||
| Sudan, R., Bhagat, M., Gupta, S., Singh, J., & Koul, A. (2014). Iron (FeII) chelation, ferric reducing antioxidant power, and immune modulating potential of Arisaema jacquemontii (Himalayan Cobra Lily). Journal of Biomedicine and Biotechnology, 14(8), 1-7. Crossref |
||||
| Supriyadi, A., Arum, L. S., Nugraha, A. S., Ratnadewi , A. A. I., & Siswoyo, T. A. (2019). Revealing antioxidant and antidiabetic potency of melinjo (Gnetum Gnemon) seed protein hydrolysate at different stages of seed maturation. Current Research in Nutrition and Food Science, 7(2), 479-487. Crossref |
||||
| Taga, M. S., Miller, E. E., & Pratt, D. E. (1984). Chia seeds as a source of natural lipid antioxidants. Journal of the American Oil Chemists' Society, 61, 928-931. Crossref |
||||
| Treml, J., & Smejkal, K. (2016). Flavonoids as potent scavengers of hydroxyl radicals. Comprehensive Reviews in Food Science and Food Safety, 15(4), 720-738. Crossref |
||||
| Yaqoob, S., Sultana, B., Mushtaq, M. (2014). In vitro antioxidant activities of Trianthema portulacastrum L. Hydrolysates. Nutrition and Food Science, 19(1), 27-33. Crossref |
||||
| Lin, H.Y., Kuo, Y.H., Lin, Y.L., & Chiang, W. (2009). Antioxidative Effect and Active Components from Leaves of Lotus (Nelumbo nucifera). Journal of Agricultural and Food Chemistry, 57(15), 6623-6629. Crossref |
||||
| Zheng, W., & Wang, S. Y. (2001). Antioxidant activity and phenolic compounds in selected herbs. Journal of Agricultural and Food Chemistry, 49(11), 5165-5170. Crossref |
||||
| Zubair, M., Anwar, F., Ali, S., & Iqbal, T. (2012). Proximate composition and mineral profile of selected rice (Oryza sativa L.) varieties of Pakistan. Asian Journal of Chemistry, 24(1), 417-421. | ||||
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