JOURNAL OF NEW DISCOVERY IN MICROBIOLOGY
Integrity Research Journals
ISSN: 3122-0207
Model: Open Access/Peer Reviewed
DOI: 10.31248/JNDM
Start Year: 2020
Email: jndim@integrityresjournals.org
Conjugative plasmid transfer among CTX-M-15 Klebsiella pneumoniae clinical isolates
https://doi.org/10.31248/JNDM2023.014 | Article Number: 31DE697B6 | Vol.2 (1) - February 2024
Received Date: 29 September 2023 | Accepted Date: 23 February 2024 | Published Date: 28 February 2024
Authors: Aisha Shitu Sa’id* , Mukhtar, M. D. , Aminu Bukar and Muhammad Yusha’u
Keywords: Antibiotic resistance, CTX-M-15, Extended Spectrum Beta Lactamase, Klebsiella pneumoniae, plasmids replicons.
Conjugational transmission of antibiotic resistance genes across bacterial species and genera has aggravated the problem of Klebsiella pneumoniae antibiotic resistance. This study aimed to determine the conjugative plasmid transfer among CTX-M-15 Klebsiella pneumoniae isolates. A total of 121 K. pneumoniae isolates were identified using conventional bacteriological techniques and production of Extended Spectrum Beta Lactamase was confirmed using double disk synergy test. Molecular detection of antibiotic resistance genes and plasmid replicons were done by PCR and plasmid based replicon typing methods respectively. Plasmid transfer capacity of the isolates harboring the CTX-M-15 genes was assessed using broth mating method. Out of the 121 isolates screened for ESBL production, only 48 (39.7%) of the K. pneumoniae isolates were ESBL producers. CTX-M-15 (n = 38, 86.4%) was the most frequently occurred ESBL gene detected. FIIK was the most prevalent replicon identified in 14(36.8%) isolates. Conjugation experiment was successful for 19(62.5%) out of 32 CTX-M-15 K. pneumoniae isolates selected as donors for conjugation based on their plasmid replicons. The replicons detected in the transconjugants included FIIK (n=10; 34.4%), FIB (n=5; 15.6%) and then FIA and R (n=4; 12.5%). A statistically significant association (P = 0.03) between the presence of CTX-M-15 and plasmid replicons was observed in this study. The spread of blaCTX-M-15 gene is driven mainly by the horizontal transfer of IncF group of plasmids which is inferred from the predominance of these plasmids among the isolates.
| Agyepong, N., Govindena, U., Owusu-Oforib, A., Amoakoa,c, D. G., Allamd, M., Janicee, J., et al. (2019). Genomic characterization of multidrug-resistant ESBL-producing Klebsiella pneumoniae isolated from a Ghanaian teaching hospital. International Journal of Infectious Diseases, 85, 117-123. Crossref |
||||
| Al-Marzooq, F., Mohd, M. Y. Tay, S. T. (2015). Molecular analysis of antibiotic resistance determinants and plasmids in malaysian isolates of multidrug resistant Klebsiella pneumoniae. PLoS ONE, 10(7), e0133654. Crossref |
||||
| Barlow, M. (2009). What antimicrobial resistance has taught us about horizontal gene transfer? Methods in Molecular Biology (Clifton, N.J.), 532, 397-411. Crossref |
||||
| Caneiras, C., Lito, L., Melo-Cristino, J., & Duarte, A. (2019). Community- and hospital-acquired Klebsiella pneumoniae urinary tract infections in Portugal: Virulence and antibiotic resistance. Microorganisms, 7, 138. Crossref |
||||
| Canton, R., Morosini, M. I., delaMaza, O. M., & delaPedrosa, E. G. (2007). IRT and CMT beta lactamases and inhibitor resistance. Clinical Microbiology and Infection, 14(1), 53-62. Crossref |
||||
| Cao, X., Xu, X., Zhang, Z., & Shen, H. (2014). Molecular characterization of clinical multidrug resistant Klebsiella pneumoniae isolates. Annual Clinical Microbiology and Antimicrobial, 13, 16. Crossref |
||||
| Carattoli, A., Bertini, A., Villa, L., Falbo, V., Hopkins, K. L., & Threlfall, E. J. (2005). Identification of plasmids by PCR-based replicon typing. Journal of Microbiological Methods, 63, 219-228. Crossref |
||||
| Clinical and Laboratory Standard Institute (CLSI) (2021). Performance standards for antimicrobial susceptibility testing. Twenty-four Informational supplements. CLSI Document 2021; M100-S24, CLSI, Wayne, Pa, USA. | ||||
| Coelho, A., González-López, J. J., Miró, E., Alonso-Tarrés, C., Mirelis, B., Larrosa, M. N., Bartolomé, R. M., Andreu, A., Navarro, F., Johnson, J. R., & Prats, G. (2010). Characterisation of the CTX-M-15-encoding gene in Klebsiella pneumoniae strains from the Barcelona metropolitan area: plasmid diversity and chromosomal integration. International journal of antimicrobial agents, 36(1), 73-78. Crossref |
||||
| Conlan, S., Park, M., Deming, C., Thomas, P. J., Young, A. C., Coleman, H., Sison, C., NISC Comparative Sequencing Program, Weingarten, R. A., Lau, A. F., Dekker, J. P., Palmore, T. N., & Frank, K. M., & Segre, J. A. (2016). Plasmid dynamics in KPC-positive Klebsiella pneumoniae during long-term patient colonization. mBio 7(3). Crossref |
||||
| Dolejska, M., Brhelova, E., Dobiasova, H., Krivdova, J., Jurankova, J., Sevcikova, A., Dubska, L., Literak, I., Cizek, A., Vavrina, M., & Sterba, J. (2012). Dissemination of IncFIIK-type plasmids in multiresistant CTX-M-15-producing Enterobacteriaceae isolates from children in hospital paediatric oncology wards. International journal of antimicrobial agents, 40(6), 510-515. Crossref |
||||
| Ferreira, R. L., Da Silva, B. C., Rezende, G. S., Nakamura-Silva, R., Pitondo-Silva, A., Campanini, E. B., Brito, M. C., da Silva, E. M., Freire, C. C. D. M., Cunha, A. F. D., & Pranchevicius, M. C. D. S. (2019). High prevalence of multidrug-resistant Klebsiella pneumoniae harboring several virulence and β-lactamase encoding genes in a Brazilian intensive care unit. Frontiers in Microbiology, 9, 3198. Crossref |
||||
| Gharrah, M. M., Mostafa El-Mahdy, A., & Barwa, R. F. (2017). Association between virulence factors and extended spectrum beta-lactamase producing Klebsiella pneumoniae compared to nonproducing isolates. Interdisciplinary Perspectives on Infectious Diseases, Volume 2017, Article ID 7279830, 14 pages. Crossref |
||||
| Giwa, F. J., Ige, O. T., Haruna, D. M., Yaqub, Y., Lamido, T. Z., & Usman, S. Y. (2018). Extended-Spectrum beta-lactamase production and antimicrobial susceptibility pattern of uropathogens in a Tertiary Hospital in Northwestern Nigeria. Annals of Tropical Pathology, 9(1), 11-16. Crossref |
||||
| Harajly, M., Khairallah, M. T., Corkill, J. E., Araj, G. F., & Matar, G. M. (2010). Frequency of conjugative transfer of plasmid-encoded ISEcp1-blaCTX-M-15 and aac (6')-lb-cr genes in Enterobacteriaceae at a tertiary care center in Lebanon-role of transferases. Annals of Clinical Microbiology and Antimicrobials, 9, Article number 19. Crossref |
||||
| Hawkey, J., Wyres, K. L., Judd, L. M., Harshegyi, T., Blakeway, L., Wick, R. R., Jenney, A. W., & Holt, K. E. (2022). ESBL plasmids in Klebsiella pneumoniae: diversity, transmission and contribution to infection burden in the hospital setting. Genome Medicine, 14(1), 1-13. Crossref |
||||
| Hennequin, C., Aumeran, C., Robin, F., Traore, O., & Forestier, C. (2012). Antibiotic resistance and plasmid transfer capacity in biofilm formed with a CTX-M-15-producing Klebsiella pneumoniae isolate. Journal of Antimicrobial Chemotherapy, 67(9), 2123-2130. Crossref |
||||
| Hymavathi, R., Shanthi Kumari1, S., Swarnalatha, G., & Surekha, A. (2018). Prevalence of ESBL producing Klebsiella species isolated from respiratory samples at intensive care units. Annals of International Medical and Dental Research, 4(4), 6-10. Crossref |
||||
| Iroha, I. R., Oji, A. E., Nwakaeze, A. E., Ayogu, T. E., Afiukwa, F. N., Ejikeugwu, P., & Esimone, C. O. (2011). Strains of Klebsiella pneumoniae from intensive care unit producing CTX-M-15 extended spectrum beta-lactamases. Am. J. Microbiol, 2(2), 35-39. Crossref |
||||
| Jordt, H., Stalder, T., Kosterlitz, O., Ponciano, J. M., Top, E. M., & Kerr, B. (2020). Coevolution of host-plasmid pairs facilitates the emergence of novel multidrug resistance. Nature Ecology & Evolution, 4(6), 863-869. Crossref |
||||
| Marisa, H. Aziza, M., Mansour, W., Grami, R., Ben, A., Chatre, P., & Dahmen, S. (2015). Dissemination of multidrug resistance blaCTX-M-15/incFIIK plasmids in K. pneumoniae isolates from hospital and community acquired human infections in Tunisia. Diagnostic Microbiology and Infectious Disease, 83(3), 298-304. Crossref |
||||
| Mathers, A. J., Peirano, G., & Pitout, J. D. (2015). The role of epidemic resistance plasmids and international high-risk clones in the spread of multidrug-resistant Enterobacteriaceae. Clinical Microbiology Reviews, 28(3), 565-591. Crossref |
||||
| Minja, C. A., Shirima, G., & Mshana, S. E. (2021). Conjugative plasmids disseminating ctx-m-15 among human, animals and the environment in Mwanza Tanzania: a need to intensify one health approach. Antibiotics, 10(7), 836. Crossref |
||||
| Mohammed, Y., Gadzama, G. B., Zailani, S. B., & Aboderin, A. O. (2016). Characterization of extended-spectrum beta-lactamase from Escherichia coli and Klebsiella species from North Eastern Nigeria. Journal of Clinical and Diagnostic Research, 10(2), DC07-DC10. Crossref |
||||
| Navon-Venezia, S., Kondratyeva, K., & Carattoli, A. (2017). Klebsiella pneumoniae: a major worldwide source and shuttle for antibiotic resistance. FEMS Microbiology Reviews, 41(3), 252-275. Crossref |
||||
| Odewale, G., Jibola-Shittu, M. Y., Ojurongbe, O., Olowe, R. A., & Olowe, O. A. (2023). Genotypic Determination of Extended Spectrum β-Lactamases and Carbapenemase Production in Clinical Isolates of Klebsiella pneumoniae in Southwest Nigeria. Infectious Disease Reports, 15(3), 339-353. Crossref |
||||
| Partridge, S. R., Zong, Z., & Iredell, J. R. (2011). Recombination in IS26 and Tn2 in the evolution of multiresistance regions carrying bla CTX-M-15 on conjugative IncF plasmids from Escherichia coli. Antimicrobial Agents and Chemotherapy, 55(11), 4971-4978. Crossref |
||||
| Podschun, R., & Ullmann, U. (1998). Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clinical Microbiology Reviews, 11(4), 589-603. Crossref |
||||
| Ramirez, M. S., Traglia, G. M., Lin, D. L., Tran, T., & Tolmasky, M. E. (2014). Plasmid-mediated antibiotic resistance and virulence in gram-negatives: The Klebsiella pneumoniae paradigm. Microbiology Spectrum, 2(5), 10-1128. Crossref |
||||
| Sa'id, A. S., Mukhkar, M. D., Bukar, A., & Yusha'u, M. (2020). Extended spectrum beta-lactamase production, biofilm formation and antibiotic resistance in clinical isolates of Klebsiella pneumoniae. Nigerian Journal of Microbiology, 34(1), 4874-4883. | ||||
| Soge, O. O., Queenan, A. M., Ojo, K. K., Adeniyi, B. A., & Roberts, M. C. (2006). CTX-M-15 extended-spectrum β-lactamase from Nigerian Klebsiella pneumoniae. Journal of Antimicrobial Chemotherapy, 57(1), 24-30. Crossref |
||||
| Thomas, C. M., & Nielsen, K. M. (2005). Mechanisms of, and barriers to, horizontal gene transfer between bacteria. Nature Reviews Microbiology, 3(9), 711-721. Crossref |
||||
| Villa, L., García-Fernández, A., Fortini, D., & Carattoli, A. (2010). Replicon sequence typing of IncF plasmids carrying virulence and resistance determinants. Journal of Antimicrobial Chemotherapy, 65(12), 2518-2529. Crossref |
||||
| World Health Organisation (WHO) (2017). Global Priority List of Antibiotic-Resistant Bacteria to Guide Research, Discovery, and Development of New Antibiotics. Retrieved 20th February 2021 from https://remed.org/wp-content/uploads/2017/03/lobal-priority-list-of-antibiotic-resistant-bacteria-2017.pdf. | ||||
| Wyres, K. L., Hawkey, J., Hetland, M. A., Fostervold, A., Wick, R. R., Judd, L. M., Hamidian, M., Howden, B. P., Löhr, I. H., & Holt, K. E. (2019). Emergence and rapid global dissemination of CTX-M-15-associated Klebsiella pneumoniae strain ST307. Journal of Antimicrobial Chemotherapy, 74(3), 577-581. Crossref |
||||
| Xu, H., Huo, C., Sun, Y., Zhou, Y., Xiong, Y., Zhao, Z., Zhou, Q., Sha, L., Zhang, B., & Chen, Y. (2018). Emergence and molecular characterization of multidrug-resistant Klebsiella pneumoniae isolates harboring bla CTX-M-15 extended-spectrum β-lactamases causing ventilator-associated pneumonia in China. Infection and Drug Resistance, 12, 33-43. Crossref |
||||
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