JOURNAL OF NEW DISCOVERY IN MICROBIOLOGY
Integrity Research Journals
Model: Open Access/Peer Reviewed
DOI: 10.31248/JNDM
Start Year: 2020
Email: jndim@integrityresjournals.org
Determination of antibiotic susceptibility pattern and multidrug resistance uropathogenic isolates of E. coli and Klebsiella sp against the commonly prescribed antibiotics
https://doi.org/10.31248/JNDM2023.013 | Article Number: 29038EEA2 | Vol.2 (2) - April 2024
Received Date: 26 September 2023 | Accepted Date: 06 February 2024 | Published Date: 30 April 2024
Authors: Shamsu I Abdullahi* , Adeshina GO , Onaolapo GA , Yusha’u M and Ali M
Keywords: antibiotics, Escherichia coli, Klebsiella, Kano, susceptibility pattern
The emergence of antibiotic resistance in the management of urinary tract infection (UTI) is a serious public health issue worldwide particularly in the developing countries. The study was aimed to determine antibiotic susceptibility pattern and multidrug resistance uropathogenic isolates of E. coli and Klebsiella sp against the commonly prescribed antibiotics. A total of one hundred and thirty-one (131) suspected Klebsiella sp. and E coli isolates were collected from the Microbiology laboratory of all the 3 study sites i.e., Murtala Muhammad Specialist Hospital (MMSH), Muhammad Abdullahi Wase Teaching Hospital (MAWTH) and Aminu Kano teaching hospital (AKTH) all in Kano State, North West Nigeria. Isolates were subjected to Gram staining, morphological and biochemical characterization as well as MicrogenTM Gram negative Identification A system. Antibiotic susceptibility testing was conducted using modified Kirby-Bauer disc- diffusion method. Result showed that all the isolates were susceptible to gentamicin (100%), 75% and 67% susceptible to ciprofloxacin and amoxicillin-clavulanic acid respectively. The resistance pattern of the isolates was observed to be cephalothin (77.5%), cefpodoxime (72.5%), ampicillin (57.5 %), tetracycline (52.5%), sulfamethoxazole-trimethoprim (50%), ceftriaxone (40%), amoxicillin-clavulanic acid (32.5%) and ciprofloxacin (25%). Klebsiella sp. showed higher resistance to cephalexin (90%), ampicillin (90%), cefpodoxime (80%), and then tetracycline (60%) while resistance of E. coli to cephalexin, cefpodoxime, and tetracycline were 73.3%, 70%, and 50% respectively. Out of the 22 MDR isolates, 14 (63.6%) were E. coli and 8 (36.4%) were Klebsiella sp. Eight (8) (20 %) were XDR with no PDR strain detected. Eight (8) of these XDR were E. coli while 6 were Klebsiella sp.
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