JOURNAL OF BIOSCIENCE AND BIOTECHNOLOGY DISCOVERY
Integrity Research Journals
ISSN: 2536-7064
Model: Open Access/Peer Reviewed
DOI: 10.31248/JBBD
Start Year: 2016
Email: jbbd@integrityresjournals.org
Entomocidal activity of Alstonia boonei de wild oil extracts against cowpea beetle, Callosobruchus maculatus fabricius (Coleoptera: Bruchidae) infesting stored cowpea seeds
https://doi.org/10.31248/JBBD2025.238 | Article Number: FB95E2E21 | Vol.10 (4) - October 2025
Received Date: 23 July 2025 | Accepted Date: 30 August 2025 | Published Date: 30 October 2025
Authors: Olusola Michael Obembe* , Mary Tejumade Philip-Attah and Mary Adeola Adegbola
Keywords: Callosobruchus maculatus, mortality, oviposition, Adult emergence, Alstonia boonei, entomocidal
This study was conducted to investigate the entomocidal effects of the oil extracts of Alstonia boonei plant parts (root barks, stem barks, leaves, and seeds) as contact insecticides against the activities of Callosobrochus maculatus in stored cowpea seeds. Air-dried parts of A. boonei were ground into fine powder using an electric grinder (Model: Binatone electric grinder BL 400). The plant parts were extracted with a Soxhlet extractor at 60°C in the laboratory, using ethanol as the solvent. The efficacy of the oil extracts was investigated on the weevils for adult mortality, oviposition, emergence, viability, and seed damage. The oil extracts were applied at the rate of 1.0 mL/20 g of cowpea seeds. The results obtained showed that all oil dosages (1 mL/20g) of the different parts of the plant used caused mortality of C. maculatus, which increased with increased time of exposure. The stem bark oil was able to achieve 100 ± 0.00 % mortality within 72 h post-treatment, while other plant parts' oil extracts achieved 100 ± 0.00% mortality within 96 h. Oviposition and adult emergence by adult C. maculatus in the treated seeds were significantly lower than those of the control. There was no egg laid in the seeds treated with stem bark oil extracts. There was no adult emergence in all the treated cowpea seeds. The percentage damage and weight loss of cowpea seeds exposed to different oil dosages of A. boonei were significantly (p ≤ 0.05) lower than that of the untreated seeds. After 7 days of germination, all the treated grains recorded high percentage germination ranging from 95.25 ± 2.33% to 100 ± 0.00%. The seed bark and leaf oil extracts were able to record 100 ± 0.00% germination. The results obtained from this study showed that oil extracts of A. boonei were effective in controlling C. maculatus.
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