JOURNAL OF AGRICULTURAL SCIENCE AND PRACTICE
Integrity Research Journals
ISSN: 2536-7072
Model: Open Access/Peer Reviewed
DOI: 10.31248/JASP
Start Year: 2016
Email: jasp@integrityresjournals.org
Inhibition effect of cypermethrin insecticide on soil fungal and bacterial species
https://doi.org/10.31248/JASP2024.455 | Article Number: A3EA24721 | Vol.9 (5) - October 2024
Received Date: 20 February 2024 | Accepted Date: 20 April 2024 | Published Date: 30 October 2024
Authors: G. A. Abubakar* and Akinsuyi Faizah
Keywords: bacteria, fungi, soil., microbes, Insecticide, cypermethrin
Insecticides are commonly used in integrated weed management programs in agricultural plantations. Their usage not only controls the weed populations but also affects microbial populations especially bacteria and fungi in soil, and hence modify soil biochemical and biological processes critical for ecosystem functioning. This study determined the effect of inorganic insecticide (Cypermethrin) on soil fungal and bacterial populations. Soil samples were contaminated with Cypermethrin for 14 days, and the microbial count, isolation, identification, and antimicrobial analysis were carried out using standard methods. The control (uncontaminated soil sample) recorded the highest bacterial count (90.67±0.06×10-6 cfu/g) as well as fungal count (5.11±0.01×10-6 cfu/g) than the Cypermethrin treated soil samples (22.33±0.02×10-6 and 4.34±0.03×10-6 cfu/g) for bacterial and fungal counts respectively, which significantly lower organic residues decomposition to release soil nutrients in soils. The isolated bacteria species from the soil samples were identified as Bacillus cereus, Bacillus pumilus, Staphylococcus aureus, Bacillus lentus, Bacillus megatarium and Bacillus femulus while the isolated fungi were identified as Aspergillus flavus, Aspergillus niger, Fusaruim chlamidosporium and Fusaruim solain. The in vitro anti-bacterial activity of the insecticide shows considerable level of inhibition against all the test organisms exhibiting its highest activity at 2.0 ml against Bacillus megatarium (29.16±0.14mm) and the least against Staphylococcus aureus (13.50±0.03 mm). Cypermethrin caused significant inhibition of fungal growth at Day 3, recorded the highest % inhibition fungal growth (56.14%) against Fusaruim chlamidosporium and least % inhibition (16.12%) was recorded against Fusaruim solain. This study suggested that insecticide applications in agricultural plantations significantly reduced the mineralization rate as well as growth and development of both bacterial and fungal species in soil.
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