JOURNAL OF AGRICULTURAL SCIENCE AND PRACTICE
Integrity Research Journals
ISSN: 2536-7072
Model: Open Access/Peer Reviewed
DOI: 10.31248/JASP
Start Year: 2016
Email: jasp@integrityresjournals.org
Isolation of Escherichia coli o157:h7 from water sources in the livestock complex, Mando, Kaduna
https://doi.org/10.31248/JASP2016.007 | Article Number: 466693522 | Vol.1 (1) - April 2016
Received Date: 26 January 2016 | Accepted Date: 28 February 2016 | Published Date: 30 April 2016
Authors: Abdulkadir A.* , Muhammad T. I. , El Yakub I. , Taru I. A. , Abba M. , Enesi L. , Bello L. and Mohammed F. I.
Keywords: EHEC, Enterobacteriaceae, reservoir, water, O157:H7.
Failure in understanding the importance of water quality exposes humans and animals to the risk of diseases. Microbial contamination remains a critical risk factor in useable water in many parts of the world. This study was aimed at investigating Escherichia coli contamination of water samples. Fifty water samples were analysed to detect the occurrence of potentially pathogenic bacteria of Enterobacteriaceae family. All isolates detected were screened biochemically using Microbact GNB 12E and serotyped for the virulence antigens O and H. The results showed 14% (7/50) of the samples were positive for Escherichia coli, Salmonella arizonae, Enterobacter gergoviae, Enterobacter aerogenes, and Hafnia alvei. Out of which 3 samples were positive for Escherichia coli isolates which were collected from borehole (2 samples) and well water (1 sample) sources. All 2 isolates were serotyped for virulence antigen O157: H7 in which only 2 serotypes were identified for O157:H7 (2%) and O157:H- (4%). Hence, it could be concluded that water may be an important reservoir for E. coli infection and thus, the risk of contracting Enterohaemorrhagic Escherichia coli (EHEC) infection from contaminated water have been clearly established.
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