Global Journal of Fisheries Science

GLOBAL JOURNAL OF FISHERIES SCIENCE
Integrity Research Journals

ISSN: 2782-750X
Model: Open Access/Peer Reviewed
DOI: 10.31248/GJFS
Start Year: 2018
Email: gjfs@integrityresjournals.org

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Investigation of bacterial organisms associated with milt of wild broodstock Clarias gariepinus (Burchell, 1822) Caught from Ogun River

https://doi.org/10.31248/GJFS2024.068   |   Article Number: 7D91451E3   |   Vol.7 (1) - February 2025

Received Date: 22 December 2024   |   Accepted Date: 06 February 2025  |   Published Date: 28 February 2025

Authors:  Awe, F. Adekunle* , Hammed, A. Mutalib , Onafadeji, O. Oluwafemi , Akinyemi, A. Adeolu , Olanloye, O. Adebimpe and Ogunbanwo, O. Michael

Keywords: bacteria, molecular characterization, wild catfish.

ABSTRACT

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This study was carried out to isolate and identify the bacteria species that are found in wild Clarias gariepinus. Identification of bacterial organisms from catfish provides updated information on emerging and existing organisms thereby enriching the gene bank on fish disease. Catfish broodstock was collected from fishermen of Ogun River located in the heart of Abeokuta, Ogun State, Nigeria.  Fish samples were taken to the Microbiology Laboratory, Federal University of Agriculture, Abeokuta Ogun State for milt microbial analyses, amplicons were taken to the International Institute of Tropical Agriculture, Ibadan for molecular characterization. Bio-edit was used for importing and mining nucleotide sequences into the gene bank. The result revealed that the following bacterial organisms were present in the milt of Clarias gariepinus: Bacillus subtilis, Aeromonas caviae and Aeromonas veronii. The Basic Local Alignment Search Tools revealed the percentage similarity ranging from 78.60 to 98.81% and their accession numbers. These bacteria indicate high levels of faecal contamination in the environment. In conclusion, bacteria were found in the milt of wild catfish.

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