ISSN: 2992-4928
Model: Open Access/Peer Reviewed
DOI: 10.31248/AJPB
Start Year: 2019
Email: ajpb@integrityresjournals.org
https://doi.org/10.31248/AJPB2024.034 | Article Number: 8D8DB69D3 | Vol.4 (1) - June 2024
Received Date: 30 April 2024 | Accepted Date: 29 June 2024 | Published Date: 30 June 2024
Authors: James Odianosen Oseyomon* , Philip A. Obarisiagbon and Odunayo Esther Omotuyi
Keywords: toxicity, methanol extract, Hyptis suaveolens, mice and rats
The present study was aimed at evaluating the acute and sub-acute toxicity potentials of methanol extract of the leaves of Hyptis suaveolens using mice and Wister rats. Using the modified Lorke’ method for the acute toxicity investigation, twelve mice received single doses of 10, 100 and 1000 mg/kg body weight respectively in the first phase. Absence of death led to the second phase, using different mice, and 5000, 7500 and 10,000 mg/Kg doses of the extract respectively was administered to one (1) mouse each. Gross toxicological symptoms and possible death in the animals were monitored for 14 days to ascertain the oral median lethal dose (LD50) of the extract. In the sub-acute investigation, the rats were given oral doses of 250, 500 and 750 mg/Kg/day for 28 consecutive days. Then, haematological and biochemical analyses were carried out. The kidneys, livers, spleens, lungs and hearts of the rats were subjected to histological studies. Results obtained showed that there were no signs of toxicity up to an acute maximum dose of 10,000 mg/Kg. Sub-acute treatment did not significantly alter animal body weights, organ-to-body weight ratios, haematological and biochemical parameters (p<0.05). However, the levels of monocyte were significantly increased and immunoglogbin decreased at a dose of 750 mg/kg. The concentration of alkaline phosphatase (ALP) level was also significantly increased while creatinine was decreased (p<0.001) at a dose of 500 and 750 mg/Kg/day, when compared with the control. Histological evaluations of the studied organs in the treated rats showed no changes when compared to the control. However, the liver showed normal hepatocytes with feature of necrosis while the lungs showed dilated alveoli spaces, desquamated cells and acute inflammation. At the treatment doses (250 - 750 mg/Kg), the stomach revealed atrophy of gastric mucosa and gastric glands while destruction of inflammatory cells from necrosis was observed in the spleen at 750 mg/Kg only. Conclusively, the methanol leaf extract of Hyptis suaveolens is relatively safe sub-acutely at low doses while high doses should be discouraged.
Akinmoladun, A. C., Ibukun, E. O., Afor, E., Obuotor, E. M., & Farombi, E. O. (2007). Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimum gratissimum. Scientific Research and Essay, 2(5), 163-166. | ||||
Arnason, J. T. (1995). Bioactive products from Mexican plants, phytochemistry of medicinal plants. Springer. https://doi.org/10.1007/978-1-4899-1778-2 |
||||
Bautista, A. R. P. L., Moreira, E. L. T., Batista, M. S., Miranda, M. S., & Gomes, I. C. S. (2004). Subacute toxicity assessment of annatto in rat. Food and Chemical Toxicology, 42(4), 625-629. https://doi.org/10.1016/j.fct.2003.11.007 |
||||
Chiamaka, O. S., Ndarubu, T. A., Mahmood, F. A., Adenike, A. R., Alfa, S., Praise, O. O., & Bashir, L. (2019). In vitro antioxidants, antimicrobials and biochemical response of methanol leaf extract of eucalyptus camaldulensis following sub-acute administration to rats. Saudi Journal of Biomedical Research, 4(11), 405-411 https://doi.org/10.36348/sjbr.2019.v04i11.007 |
||||
Chukwujekwu, J. C., Smith, P., Coombes, P. H., Mulholland, D. A., & Van Staden, J. (2005). Antiplasmodial diterpenoid from the leaves of Hyptis suaveolens. Journal of Ethnopharmacology, 102(2), 295-297. https://doi.org/10.1016/j.jep.2005.08.018 |
||||
Cunny, H., & Hodgson, E. (2009) Toxicity testing. In: Hodgson, E. (ed.). A textbook on modern toxicology (3rd edition). Wiley, Hoboken. Pp. 353-384. | ||||
Danmalam, U. H., Abdullahi, L. M., Agunu, A., & Musa, K. Y. (2009). Acute toxicity studies and hypoglycemic activity of the methanol extract of the leaves of Hyptis suaveolens Poit. (Lamiaceae). Nigerian Journal of Pharmaceutical Sciences, 8(2), 87-92. | ||||
Debelo, N., Afework, M., Debella, A., Makonnen, E., Ergete, W., & Geleta, B. (2015). Histopathological and biochemical assessment of chronic oral administration of aqueous leaf extract of Thymus serrulatus in mice. Journal of Clinical and Experimental Pathology, 5(6), 258. https://doi.org/10.4172/2161-0681.1000258 |
||||
Devaki, K., Beulah, U., Akila, G., & Gopalakrishnan, V. K. (2012). Effect of aqueous extract of Passiflora edulis on biochemical and hematological parameters of Wistar albino rats. Toxicology international, 19(1), 63-67. https://doi.org/10.4103/0971-6580.94508 |
||||
Edeoga, H. O., Omosun, G., & Uche, L. C. (2006). Chemical composition of Hyptis suaveolens and Ocimum gratissimum hybrids from Nigeria. African journal of Biotechnology, 5(10), 892-895. | ||||
Enenebeaku, U. E., Okotcha, E. N., Oguoma, L. M., Mgbemena, I. C., Enenebeaku, C. K., & Onyeka, C. A. (2021b). Biochemical and haematological enhancement activities of aqueous and methanol leaves, stem and roots extracts of Chasmanthera dependens (Hochst) and Dictyandra arborescens (Welw.). Bulletin of the National Research Centre, 45, Article number 186. https://doi.org/10.1186/s42269-021-00642-7 |
||||
Enenebeaku, U. E., Ukwandu, N. C., Mgbemena, I. C., Nwigwe, H. C., Enenebeaku, C. K., Duru, C. E., & Ogidi, O. I. (2021a). Oral acute toxicity and antimalarial potentials of aqueous and methanolic extracts of roots, leaves and stem of Dictyandra arborescens (Welw.) on Plasmodium berghei infected mice. Bulletin of the National Research Centre, 45, Article number 75. https://doi.org/10.1186/s42269-021-00530-0 |
||||
Gatsing, D., Aliyu, R., Kuiate, J. R., Garba, I. H., Jaryum, K. H., Tedongmo, N., Tchouanguep, F. M., & Adoga, G. I. (2005). Toxicological evaluation of the aqueous extract of Allium sativum bulbs on laboratory mice and rats. Cameroon Journal of Experimental Biology, 1(1), 39-45. https://doi.org/10.4314/cajeb.v1i1.37926 |
||||
Lorke, D. (1983). A new approach to practical acute toxicity testing. Archives of Toxicology, 54, 275-287. https://doi.org/10.1007/BF01234480 |
||||
Mozhiyarasi, P., & Anuradha, R. (2016). A study on phytochemical analysis and antimicrobial activity of Hyptis suaveolens (L.) Poit. Journal of Chemical and Pharmaceutical Research, 8(6), 438-442. | ||||
Michael, B., Yano, B., Sellers, R. S., Perry, R., Morton, D., Roome, N., Johnson, J. K., & Schafer, K. (2007). Evaluation of organ weights for rodent and non-rodent toxicity studies: a review of regulatory guidelines and a survey of current practices. Toxicologic Pathology, 35(5), 742-750. https://doi.org/10.1080/01926230701595292 |
||||
Monosson, E. (2013). Toxicity testing methods. Encyclopedia of earth topics. Retrieved 13 March 2020 from https://editors.eol.org/eoearth/wiki/Toxicity_testing_methods. | ||||
Ngozi, L. U., Ugochukwu, N., Ifeoma, P. U., Charity, E. A., & Chinyelu, I. E. (2014). The efficacy of Hyptis suaveolens: A review of its nutritional and medicinal applications. European Journal of Medicinal Plants, 4(6), 661-674. https://doi.org/10.9734/EJMP/2014/6959 |
||||
Ogbe, R. J., Adoga, G. I., & Abu, A. H. (2010). Anti anaemic potentials of some extracts on phenyl hydrazine- induced anaemia in rabbits. Journal of Medicinal Plants Research, 4(8), 680-684. | ||||
Ojo, O. A., Oloyede, O. I., Olarewaju, O. I., Ojo, A. B., Ajiboye, B. O., & Onikanni, S. A. (2013). Toxicity studies of the crude aqueous leaves extracts of Ocimum gratissimum in albino rats. IOSR J Environ Sci Toxicol Food Technol, 6(4), 34-39. https://doi.org/10.9790/2402-0643439 |
||||
Ozolua, R. I., & Uwaya, D. O. (2013). Laboratory-based safety assessment tests of some Nigerian commercial herbal products. Journal of Pharmacovigilance S1, 001. https://doi.org/10.4172/2329-6887.S1-001 |
||||
Ozolua, R., Anaka, O., Okpo, S., & Idogun, S. (2009). Acute and sub-acute toxicological assessment of the aqueous seed extract of Persea americana Mill (Lauraceae) in rats. African Journal of Traditional, Complementary and Alternative Medicines, 6(4), 573-578. https://doi.org/10.4314/ajtcam.v6i4.57214 |
||||
Ozolua, R. I., Idogun, S. E., & Tafamel, G. E. (2010). Acute and sub-acute toxicological assessment of aqueous leaf extract of bryophyllum pinnatum (Lam.) in Sprague-Dawley rats. American Journal of Pharmacology and Toxicology, 5(3), 145-151 https://doi.org/10.3844/ajptsp.2010.145.151 |
||||
Parandın, R., & Rohanı, S. H. (2010). Effect of the oil extract of Ocimum gratissimum leaves on the reproductive function and fertility of adult male rats. Journal of Applied Biological Sciences, 4(2), 1-4. | ||||
Parasuraman, S., Thing, G. S., & Dhanaraj, S. A. (2014). Polyherbal formulation: Concept of ayurveda. Pharmacognosy Reviews, 8(16), 73-80. https://doi.org/10.4103/0973-7847.134229 |
||||
Pearce, E. L., Poffenberger, M. C., Chang, C. H., & Jones, R. G. (2013). Fueling immunity: insights into metabolism and lymphocyte function. Science, 342(6155), 1242454. https://doi.org/10.1126/science.1242454 |
||||
Prasanna, S. R., & Koppula, S. B. (2012). Antimicrobial and preliminary phytochemical analysis of solvent extracts of Hyptis suaveolens from banks of River Krishna. International Journal of Bio-Pharma Research, 1(1), 11-15. | ||||
Prashantkumar, P., & Vidyasagar, G. M. (2006). Documentation of traditional knowledge on medicinal plants of Bidar district, Karnataka. Indian Journal of Traditional Knowledge, 5(3), 295-299. | ||||
Raizada, P. (2006). Ecological and vegetative characteristics of a potent invader, Hyptis suaveolens Poit. from India. Lyonia: Journal of Ecology and Application, 11(2), 115-120. | ||||
Salawu, O. A., Chindo, B. A., Tijani, A. Y., Obidike, I. C., Salawu, T. A., & Akingbasote, A. J. (2009). Acute and sub-acute toxicological evaluation of the methanolic stem bark extract of Crossopteryx febrifuga in rats. African Journal of Pharmacy and Pharmacology, 3(12), 621-626. https://doi.org/10.4314/jopat.v12i1.41366 |
||||
Shakya, A. K. (2016). Medicinal plants: Future source of new drugs. International journal of herbal medicine, 4(4), 59-64. | ||||
Teo, S., Stirling, D., Thomas, S., Hoberman, A., Kiorpes, A., & Khetani, V. (2002). A 90-day oral gavage toxicity study of d-methylphenidate and d, l-methylphenidate in Sprague-Dawley rats. Toxicology, 179(3), 183-196. https://doi.org/10.1016/S0300-483X(02)00338-4 |
||||
Tousson, E., El-Moghazy, M., & El-Atrsh, E. (2011). The possible effect of diets containing Nigella sativa and Thymus vulgaris on blood parameters and some organs structure in rabbit. Toxicology and Industrial Health, 27(2), 107-116. https://doi.org/10.1177/0748233710381891 |
||||
Treasure, J. (2003). Urtica semen reduces serum creatinine levels. Journal of American Herbalists Guild, 4(2), 22-25. | ||||
Ugwah-Oguejiofor, C. J., Okoli, C. O., Ugwah, M. O., Umaru, M. L., Ogbulie, C. S., Mshelia, H. E., Umar, M., & Njan, A. A. (2019). Acute and sub-acute toxicity of aqueous extract of aerial parts of Caralluma dalzielii, Heliyon, 5, e01179. https://doi.org/10.1016/j.heliyon.2019.e01179 |
||||
Umar, S. I., Ndako, M., Jigam, A. A., Adefolalu, S. F., Ibikunle, G. F., & Lawal, B. (2019). Anti-plasmodial, Anti-inflammatory, anti-nociceptive and safety profile of Maytenus senegalensis root bark extract on hepato-renal integrity in experimental animals. Comparative Clinical Pathology, 28(6), 1571-1579. https://doi.org/10.1007/s00580-019-02965-4 |
||||
Usman, N., Ozolua, R., Uwaya, D., Osagiede, B., & Ugiagbe, E. (2016). Acute and sub-acute toxicological evaluation of aqueous leaf extract of Nauclea latifolia (Rubiaceae) in albino rats. European Journal of Medicinal Plants, 12(2), 1-10. https://doi.org/10.9734/EJMP/2016/23779 |
||||
Walmsley, S. J., Broeckling, C., Hess, A., Prenni, J., & Curthoys, N. P. (2010). Proteomic analysis of brush-border membrane vesicles isolated from purified proximal convoluted tubules. American Journal of Physiology-Renal Physiology, 298(6), F1323-F1331. https://doi.org/10.1152/ajprenal.00711.2009 |
||||
Yang, X., Schnackenberg, L. K., Shi, Q., & Salminen, W. F. (2014). Hepatic toxicity biomarkers. In: Biomakers in Toxicology. Pp. 241-259. https://doi.org/10.1016/B978-0-12-404630-6.00013-0 |
||||
Yusuf, A. A., Garba, R., Alawode, R. A., Adesina, A. D., Oluwajobi, I., Ariyeloye, S. D., Agboola, R.A., Salisu, L., Abubakar, S., Dan-Mallam, U., & Berinyuy, B. E. (2020). Alterations in serum urea, creatinine, and electrolytes concentrations in wistar rats following repeated administration of methanol extracts of Azanza garckeana Pulp. Noble International Journal of Agriculture and Food Technology, 2(1), 1-7. |