Received Date: 11 August 2023 | Accepted Date: 26 August 2023 | Published Date: 30 October 2023
Keywords: parasitaemia, Andrographis paniculata, biochemical parameters, trypanosomiasis.
The goal of the study was to ascertain the in vivo anti-trypanosomal activity of methanol extract from leaves of Andrographis paniculata and its impact on a number of biomarkers in Trypanosoma brucei-infected mice. Six groups of three mice each were created at random from a total of eighteen mice. As negative and positive control, Groups I and II received normal saline (2 ml/kg body weight) and diminazene aceturate (3.5 mg/kg body weight), respectively. Groups III-V were treated with leaf extract at doses of 150, 300, and 600 mg/kg body weight, respectively. The oral route was used for all treatments. The average body weight, survival time, and daily parasitemia level were assessed. Alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total protein concentration in mouse serum and liver were also assessed. Phytochemical screening was done. Flavonoids, saponins, glycosides, alkaloids, tannins, and phenols were all present in the leaves of Andrographis paniculata. The extract was relatively safe according to acute toxicity tests. When compared to untreated mice, the extract at 600 mg/kg body weight considerably increased mice body weight and survival rates while showing negligible trypanostatic potential. The serum, liver ALP and AST activities of infected increased significantly (p < 0.05) compared to untreated mice. The activities of these enzymes were significantly (p < 0.05) recovered after treatment with methanol extract of leaves from Andrographis paniculata. The serum AST and liver ALT activities, however, did not significantly change. Although Andrographis paniculata showed little trypanostatic efficacy, it considerably ameliorated the metabolic changes in mice caused by T. brucei.
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