JOURNAL OF DRUGS AND PHARMACEUTICAL SCIENCE
Integrity Research Journals

ISSN: 2705-2222
Model: Open Access/Peer Reviewed
DOI: 10.31248/JDPS
Start Year: 2016
Email: jdps@integrityresjournals.org


Neurobehavioural effects of Vigna unguiculata spp Dekindtiana (L.) Walp in mice

https://doi.org/10.31248/JDPS2019.021   |   Article Number: F8A884823   |   Vol.3 (3) - August 2019

Received Date: 11 June 2019   |   Accepted Date: 09 July 2019  |   Published Date: 30 August 2019

Authors:  Adekunle Theophilus ADEGBUYI* , Moses Atanda AKANMU and Gbola OLAYIWOLA

Keywords: Grooming, locomotion, novelty-induced behavior, phenobarbital-induced sleeping time, rearing, Vigna unguiculata spp dekindtiana.

The study investigated the pharmacological effects of the extract and fractions of Vigna unguiculata spp dekindtiana. The neurobehavioural parameters investigated in this study include: rearing, grooming and locomotion as well as pentobarbital-induced sleeping time model as a measure of the sedative effect of the plant. The neuropharmacological profile of the plant was determined in mice. The extract and fractions were administered orally at doses of 100, 200, 400 and 800 mg/kg for novelty-induced behavior while phenobarbital-induced sleeping time was assessed at the doses of 100, 200 and 400 mg/kg. The result obtained showed significant decrease in rearing, grooming and locomotion with increase in the dose of the extract and the fractions indicating the central inhibitory effect of the plant extract. The possible mechanism of action of the novelty-induced behaviours was investigated by using picrotoxin (GABAA antagonist), cyproheptadine (5HT2 antagonist), yohimbine (alpha2 adrenergic antagonist). The result of the study showed the reversal of the novelty-induced rearing and locomotion with picrotoxin while the reduction in novelty-induced behaviour (rearing and locomotion) was potentiated by both cyproheptadine and yohimbine. This shows the possibility of GABAergic, serotonergic and adrenergic involvement in the behavioural effects of the extract and its fractions. Also, the methanol extract and ethyl acetate fraction showed dose-dependent increase in sleeping time and dose-dependent decrease in sleep latency indicating the hypnotic effect of the extract of the plant. The possible mechanism of action of the phenobarbital-induced sleeping time was investigated using flumazenil (an antagonist of GABA), where a reversal was obtained suggesting the possible involvement of GABAergic receptor. In conclusion, the result showed that methanol extract and its fractions exhibited both central inhibitory effect and hypnotic effect in mice.

Aderibigbe, A. O., Iwalewa, E. O., Adesina, S. K., & Agboola, O. I. (2010). Studies of behavioural and neural mechanisms of aridanin isolated from Tetrapleura tetraptera in mice. International Journal of Pharmacology, 6(4), 480-486.
Crossref
 
Aduema, W., Azunna, U., & Agbai, J. U. (2017). Analgesic activity of vigna unguiculata in CD-1 mice following formalin administration. Continental Journal of Biological Sciences, 10(2), 1-9.
Crossref
 
Ajayi, A. A., & Ukponmwan, O. E. (1994). Evidence of Angiotensin II and endogenous opioid modulation of novelty-induced rearing in the rat. African Journal of Medicine and Medical Sciences, 23, 287-290.
 
Akinpelu, L. A., Adegbuyi, T. A., Agboola, S. S., Olaonipekun, J. K., Olawuni, I. J., Adegoke, M. A., Oyemitan, I. A., & Olayiwola, G. (2017). Antidepressant activity and mechanism of aqueous extract of Vigna unguiculata ssp.dekindtiana (L.) Walp dried aerial part in mice. International Journal of Neuroscience and Behavioral Science, 5(1), 7-18.
 
Ayan, I., Mut, H., Basaran, N., Acar, Z., & Ascl, O. O. (2012). Forage potentials of cowpea (Vigna unguiculata L. Walp). Turkish Journal of Field crops, 17(2), 135-138.
 
Barnes, N. M., & Sharp, T. (1999). A review of central 5-HT receptors and their function. Neuropharmacology, 38(8), 1083-1152.
Crossref
 
Erden, B. F., Ulak, G., Yildiran, G., & Gacar, N. (1997). The effect of 7-nitro indazole on pentobarbital-induced sleep in mice. Pharmacological research, 36(4), 265-267.
Crossref
 
Falodun, A. T., Ajayi, C. O., Obuotor, E. M., Adepiti, A. O., Akanmu, M. A. and Elujoba, A. A. (2015). Antiplasmodial properties, toxicity and novelty-induced behavior of a formulation from Picralima nitida and Alstonia boonei. European Journal of Medicinal Plants, 8(2), 112-120.
Crossref
 
Fery, F. L., (2002). New opportunities in Vigna. In: Janick. J., & Whipkey A. (eds.), Trends in new crops and new uses. ASHS press, Alexandria V. A. Pp. 424-428.
 
Fisher, S. P., Godinho, S. I. H., Pothecary, C. A., Hankins, M. W., Foster, R. G., & Peirson, S. N. (2012). Rapid assessment of sleep/wake behaviour in mice. Journal Biological Rhythms, 27(1), 48-58.
Crossref
 
Gangarao, B., Anjana Male, Ch. K. V. L. S. N., Hari Priya, T., Venna, N. M., Reeshma, S. K. (2011). A phytopharmacological review on vigna species. Pharmanest an International Journal of Advances in Pharmaceutical Sciences, 2(1), 62-67.
 
Gupta, U. K., Das, S., Aman, S., & Nayak, A. (2016). Pharmacological activities of Vigna unguiculata- a review. World Journal of Pharmaceutical Research, 5(10), 337-345.
 
Haberzettl, R., Fink, H., & Bert, B. (2014). Role of 5-HT1A- and 5-HT2A receptors for the murine model of the serotonin syndrome. Journal of Pharmacological and Toxicological Methods, 70(2), 129-133.
Crossref
 
Hanell, A., & Marklund, N. (2014). Structured evaluation of rodent behavioral tests used in drug discovery research. Frontiers in Behavioral Neuroscience, 8(article 252), 1-13.
Crossref
 
Hasan, S. M. R., Hossain, M., Akter, R., Mariam, J., Hoque Mazumder, E., & Shafiqur, R. (2009). Sedative and anxiolytic effects of different fractions of the Commelinaben ghalensis Linn. Drug Discovery Therapy; 3(5), 221-227.
 
Hershey, J. D., Gifford, J. J., Zizza, L. J., Pavlenko, D. A., Wagner, G. C., & Miller, S. (2018). Effects of Various Cleaning Agents on the Performance of Mice in Behavioral Assays of Anxiety. Journal of the American Association for Laboratory Animal Science, 57(4), 335-339.
Crossref
 
Ishyaku, M. F., & Aliyu, H. (2013). Field evaluation of cowpea Genotypes for drought tolerance and striga resistance in the dry savanna of the North-West Nigeria. International Journal of Plant Breeding and genetics, 7(1), 47-56.
Crossref
 
Johnston, G. A. (2013). Advantages of an antagonist: bicuculline and other GABA antagonists. British Journal of Pharmacology, 169(2), 328-336.
Crossref
 
Kumar, K. S., Rajesh, K., Anusha, D., & Suthakaran, R. (2014). Potentiation of phenobarbitontal-induced hypnosis by Celosia Argentea Leaves. International Journal of Medicine and Pharmaceutical Research, 2(3), 622-625.
 
Lever, C., Burton, S., & Ο'Keefe, J. (2006). Rearing on hind legs, environmental novelty, and the hippocampal formation. Reviews in the Neurosciences, 17(1-2), 111-134.
Crossref
 
Lush, W. M., Evans, L. T., & Wien, H. C. (1980). Environmental adaptation of wild and domesticated cowpeas (Vigna unguiculata (L.) Walp.). Field crops research, 3, 173-187.
Crossref
 
Millan, M. J., Newman-Tancredi, A., Audinot, A., Cussac, D., Lejeune, F., Nicolas, J. P., Coge, F., Galizzi, J. P., Boutin, J. A., Rivet, J. M., Dekevne, A., & Gobert, A. (2000). Agonist and antagonist actions of yohimbine as compared to fluparoxan at alpha(2)-adrenergic receptors (AR)s, serotonin (5-HT)(1A), 5-HT(1B), 5-HT(1D) and dopamine D(2) and D(3) receptors. Significance for the modulation of fronto-cortica monoaminergic transmission and depressive states. Synape, 35(2), 79-95.
Crossref
 
Nishio, M., Kukoki, Y., & Watanabe, Y. (2003). Role of hippocampal alpha (2A)-adrenergic receptor in methamphetamine-induced hyper-locomotion in the mouse. Neuroscience Letters, 341(2), 156-160.
Crossref
 
Olayiwola, G., Ukponmwan, O., & Olawode, D. (2013). Sedative and anxiolytic effects of the extracts of the leaves of Stachytarpheta cayennensisin mice. African Journal Traditional Complementary Alternative Medicine, 10(6), 568-579.
Crossref
 
Onigbogi, O., Ajayi, A. A., & Ukponmwan, O. E. (2000). Mechanisms of chloroquine induced body scratching behaviour in rats: Evidence of involvement of endogenous opioid peptides. Pharmacology Biochemistry Behaviour, 65(2), 333-337.
Crossref
 
Oyekunle, O. A., Akanmu, M. A., & Ogundeji, T. P. (2010). Evaluation of anxiolytic and novelty induced behaviours following bee-honey consumption in rats. Journal of Neuroscience and Behavioural Health, 2(4), 38-43.
Crossref
 
Singh, B. B., Ajeigbe, H. A., Tarawali, S. A., Fernandez-Rivera, S., & Abubakar, M. (2003). Improving the production and utilization of cowpea as food and fodder. Field Crops Research, 84(1-2), 169-177.
Crossref
 
Stanford, S. C. (2007). The open field test: Reinventing the wheel. Journal of Psychopharmacology; 21(2), 134-144.
Crossref
 
van der Staay, F. J., Arndt, S. S., & Nordquist, R. E. (2009). Evaluation of animal models of neurobehavioral disorders. Behavioral and Brain Functions, 5(11), 1-23.
Crossref
 
Walting, K. J. (1998). Overview of central nervous system receptors. The RBI Handbook of Receptor Clarification and signal transduction, 3rd edition. RBI. Natick, M. A. Pp. 2-45.