JOURNAL OF BIOSCIENCE AND BIOTECHNOLOGY DISCOVERY
Integrity Research Journals
ISSN: 2536-7064
Model: Open Access/Peer Reviewed
DOI: 10.31248/JBBD
Start Year: 2016
Email: jbbd@integrityresjournals.org
Molecular characterisation of bacteria isolated from dump site at Gbogidi, Ilaro, Ogun State, Nigeria
https://doi.org/10.31248/JBBD2024.220 | Article Number: 6437CE722 | Vol.9 (4) - October 2024
Received Date: 19 September 2024 | Accepted Date: 26 October 2024 | Published Date: 30 October 2024
Authors: Ntuen, U. R. , Etukudo, O. M.* , Bassey, N. S. , Nyoyoko, V. F. , Johnson, E. I. and Akpan, G.
Keywords: bacteria, Isolation, characterisation, dumpsite, molecular, Ogun State.
This study used both genetic and cultural methods to identify the bacterial species linked to dumpsite soil. Samples of soil were taken in Gbogigi, Ilaro, Ogun State. Both the Nutrient Agar method and the Serial Dilution approach were used to isolate the bacteria linked to the dumpsite soil. Using the Quick-DNA Fungal/Bacterial MiniPrepTM Kit, deoxyribonucleic acid (DNA) was extracted from pure cultures of bacterial isolates, and the concentration was measured with a Nanadrop 2000c Spectrophotometer. Using the universal primer pair 16SF and 16SR for bacteria, the isolated DNA's I6S rRNA gene was amplified using polymerase chain reaction (PCR). Utilizing an ABI3500 Genetic Analyzer, sequencing was done. In order to identify species and determine evolutionary trends, the sequences were aligned and compared with a subset of sequences available in the National Centre for Biotechnology Information (NCBI) database. The two types of bacteria that were found were un-cultured macrococcus sp. (KP131824.1) and Bacillus pumilus (KC844765.1). This study provided more information on the bacteria species associated with dumpsite soils and it has paved the way for the possibility of isolating species with degradation potentials and the degraded solid waste can be useful in agriculture as fertilizer.
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