Integrity Research Journals

ISSN: 2536-7064
Model: Open Access/Peer Reviewed
DOI: 10.31248/JBBD
Start Year: 2016

Role of phytohormone on in vitro regeneration of tea [Camellia sinensis (L.) O. Kuntze]   |   Article Number: 4C51E6841   |   Vol.8 (4) - August 2023

Received Date: 05 June 2023   |   Accepted Date: 04 July 2023  |   Published Date: 30 August 2023

Authors:  Mokaram Hanifa Koly , Md. Ekramul Hoque , Khadiza Khatun , Kazi Meftahul Jannat and Md. Rafiqul Islam*

Keywords: Callus induction, MS media, phytohormone, shoot regeneration, tea.

Phytohormones are small molecules present in very low concentrations in plants and provide a promising strategy to improve mass culture biotechnology due to their intrinsic role in growth, development, and survival. This study is aimed at evaluating the effect of different plant growth regulators on in vitro callus induction and shoot regeneration in tea. The experiment was conducted at Complete Randomize Design (CRD) with three replications. Callus induction and shoot regeneration was done by using Murashige & Skoog (MS) media supplemented with various concentrations and combinations of Naphthalene Acetic Acid (NAA), Benzyl Adenine (BA), and 2,4-Dichlorophenoxyacetic Acid (2,4-D). Shoot tips and nodal segments were used as explant. The highest percentage (86.51%) of callus induction was observed in the combined treatment of 2.00 milligram per litre (mg/L) BA + 1.50 mg/L 2, 4-D in 2.12 weeks. The same treatment produced the highest weight of calli, which was 0.18, 0.56, and 1.11 grams (g) for 6, 10, and 14 weeks after inoculation (WAI), respectively. The highest percentage (84.39%) of shoot induction was recorded with 2.00 mg/L BA + 2.00 mg/L NAA in 32.43 days. The maximum number (1.42, 2.46, and 4.49) and the highest length of the shoot (1.41, 3.28, and 4.61 cm) at 60, 90, and 120 DAI (days after inoculation) were also recorded from the same treatment. No callus or shoot induction was recorded in the controlled treatment. The protocol developed from the present study might be useful for the large-scale production of healthy, disease-free planting material for tea.

Agarwal, B., Singh, U., & Banerjee, M. (1992). In vitro clonal propagation of tea (Camellia sinensis (L.) O. Kuntze). Plant Cell, Tissue and Organ Culture, 30(1), 1-5.
Arulpragasam, P. V, & Latiff, R. (1986). Studies on the tissue culture of tea (Camellia sinensis (l.) O. Kuntzej. 1. Development of aculture method for the multiplication of shoots. Sri Lanka Journal of Tea Science, 55(1), 44-47.
Bangladesh Tea Board. (2023). Statistical Bulletin of Bangladesh Tea Board for the Month of January, 2023. Retrieved from
Begum, A., Ahmad, I., Prodhan, S. H., Azad, A. K., Sikder, M. B. H., & Ara, M. R. (2015). Study on in vitro propagation of tea [Camellia sinensis (L.) O. Kuntze] through different explants. Journal of Global Biosciences, 4(7), 2878-2887.
Boonerjee, S., Hoque, M. I., & Sarker, R. H. (2013). Development of in vitro micro propagation system in Tea plant [Camellia sinensis (L.) O. Kuntze] using shoot tip and nodal segment explants. Tea Journal of Bangladesh, 42, 21-30.
Iddagoda, N., Kataeva, N. N., & Butenko, R. G. (1988). In vitro clonal micropropagation of tea (Camellia sinensis L.) 1. Defining the optimum condition for culturing by means of a mathematical design technique. Indian Journal of Plant Physiolog, 31, 1-10.
Jha, T., & Sen, S. K. (1992). Micropropagation of an elite Darjeeling tea clone. Plant Cell Reports, 11(2), 101-104.
Kato, M. (1985). Regeneration of plantlets from tea stem callus. Japanese Journal of Breeding, 35(3), 317-322.
Khan, P. S. S. V., Prakash, E., & Rao, K. R. (1997). In vitro micropropagation of an endemic fruit tree Syzygium alternifolium (Wight) walp. Plant Cell Reports, 16(5), 325-328.
Kumari, M., Nee, P., & Mitra, G. C. (1984). Regeneration of tea shoots from nodal explants in tissue culture. Current Science, 53(16), 874-876.
Mondal, T. K., Bhattacharya, A., Laxmikumaran, M., & Ahuja, P. S. (2004). Recent advances of tea (Camellia sinensis) biotechnology. Plant Cell, Tissue and Organ Culture, 76(3), 195-254.
Mondal, T. K., Parathiraj, S., & Kumar, P. M. (2005). Micrografting: A technique to shorten the hardening time of micropropagated shoots of tea (Camellia sinensis (L) O. Kuntze). Sri Lanka Journal of Tea Science, 70(1), 5-9.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497.
Prakash, O., Sood, A., Sharma, M., & Ahuja, P. S. (1999). Grafting micropropagated tea [Camellia sinensis (L.) O. Kuntze] shoots on tea seedlings - A new approach to tea propagation. Plant Cell Reports, 18(10), 883-888.
Sandal, I., Bhattacharya, A., & Singh Ahuja, P. (2001). An efficient liquid culture system for tea shoot proliferation. Plant Cell, Tissue and Organ Culture, 65(1), 75-80.
Sandal, I., Kumar, A., Bhattacharya, A., Sharma, M., Shanker, A., & Ahuja, P. S. (2005). Gradual depletion of 2,4-D in the culture medium for indirect shoot regeneration from leaf explants of Camellia sinensis (L.) O. Kuntze. Plant Growth Regulation, 47(2), 121-127.
Sarwar, M. (1985). Callus formation from explanted organs of tea (Camellia sinensis L.). Journal of Tea Science, 54(1), 18-22.
Seran, T. H., Hirimburcgama, K., & Gunasckare, M. T. K. (2007). Establishment of in vitro culture to produce friable callus from leaf of Camellia sinensis (L.). Agrieast, 6, 49-58.