JOURNAL OF BIOSCIENCE AND BIOTECHNOLOGY DISCOVERY
Integrity Research Journals

ISSN: 2536-7064
Model: Open Access/Peer Reviewed
DOI: 10.31248/JBBD
Start Year: 2016
Email: jbbd@integrityresjournals.org


Assessment of commonly used Hospital Disinfectants on Bacteria isolated from the Operating Theatre

https://doi.org/10.31248/JBBD2016.014   |   Article Number: 10A13F743   |   Vol.1 (3) - August 2016

Received Date: 25 May 2016   |   Accepted Date: 23 July 2016  |   Published Date: 30 August 2016

Authors:  Obi C.* , Muhammad U. K. , Manga S. B. , Atata R. F. and Hauwa T.

Keywords: Efficacy of disinfectant, nocosomial pathogens, hospital disinfectants, operating theatre.

Assessment of commonly used hospital disinfectants on bacteria isolated from the operating theatre of Usmanu Danfodiyo University Teaching Hospital Sokoto was evaluated. Bacterial species were isolated by settling plate method, using Nutrient and MacConkey agar as the isolating medium. The isolates were fully characterized by standard methods. Isolated bacteria includes; Staphylococcus aureus, Staphylococcus epidermidis, Bacillus megaterium, Bacillus cereus, Streptococcus sp and Bacillus subtilis. The in house disinfectants (Povidone iodine and Izal) used in the operating theatres was obtained and evaluated using Agar ditch diffusion method. Povidone iodine obtained from the operating theatre showed no zones of inhibition in all the isolates tested, Izal solution obtained from the theatres showed appreciable zones of inhibition. Povidone iodine purchased from a pharmacy store in town however produced zones of inhibitions ranging from 7.75 mm to 18.00 mm. Killing rate for the most resistant isolates showed that B. megaterium, S. aureus and B. cereus required 25 to 30 minutes of exposure time for 8% Izal dilution to bring about the death of all resistant isolates. It was concluded that disinfection remain one of the most effective ways of reducing nosocomial pathogens in hospital environment as demonstrated in the results of this research work. However, from time to time potency of the in house used disinfectant must be evaluated in order to keep pace with degradation of disinfectant which normally occurs with time.

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