JOURNAL OF AGRICULTURAL SCIENCE AND PRACTICE
Integrity Research Journals
ISSN: 2536-7072
Model: Open Access/Peer Reviewed
DOI: 10.31248/JASP
Start Year: 2016
Email: jasp@integrityresjournals.org
Effectiveness of systemic and contact fungicides against Alternaria citri the causal organism of citrus brown spot disease in citrus mangroves of Pakistan
https://doi.org/10.31248/JASP2018.080 | Article Number: 4CA89B7F3 | Vol.3 (2) - April 2018
Received Date: 26 March 2018 | Accepted Date: 16 April 2018 | Published Date: 30 April 2018
Authors: Muhammad Farooq* , Memoona Siddique , Ateeq –Ur-Rehman , Moses Kwaku Golly* , Bakhshah Zib , Ismail khan , Shoaib Khan , Iltaf Khan , Muhammad Bakhtiar and Naila Ilyas
Keywords: Chemical control, disease incidence, disease severity, inhibition, mycelia growth.
Brown spot of citrus caused by Alternaria citri is among several diseases responsible for low crop production in Pakistan. Hence, the present study provides new information about the selection of chemicals for control of citrus brown spot in Pakistan. A. citri was isolated from different diseased samples of citrus plant collected from different orchards of Punjab Province of Pakistan. In vitro efficacy of fungicides was checked against A. citri. Fungicides viz; Thiophenate methyl, Difenoconazole, hexaconazole, Metalaxyl + Mancozeb, Metiram, Mancozeb, Azoxystrobin, Dodine, Chlorothalonil, Fosetyl aluminium were evaluated against tested fungus at various concentrations (25, 50, 100, 200 and 400 ppm) by using poisoned food technique. The results showed that majority of the fungicides exhibited their highest inhibitory activity at a concentration of 400 ppm. Fosetyl aluminium, Metiram, Thiophenate methyl, Mancozeb and Metalaxyl + Mancozeb all exhibited maximum inhibitory mycelial growth of A. citri with 49.55, 52.96, 65.57, 84.86 and 100% inhibtion, respectively at a concentration of 400 ppm whereas, a combination of Azoxystrobin and Difenoconazole exhibited maximum inhibitory mycelial growth of A. citri up to 94.95% at fungicide concentration of 300 ppm. Cholorthalonil, Hexaconazole, Dodine, and Difenoconazole were the most effective in inhibiting A. citri as no mycelial growth was observed at all concentrations and as such they produced 100% decreased in mycelia growth over the control. The study concluded that Fosetyl aluminium was the least effective fungicide against A. citric. The present study provides new information on the selection of chemicals for control of citrus brown spot in Pakistan.
| Alférez, F., Agusti, M., & Zacarıas, L. (2003). Postharvest rind staining in Navel oranges is aggravated by changes in storage relative humidity: effect on respiration, ethylene production and water potential. Postharvest Biology and Technology, 28(1), 143-152. Crossref |
||||
| Bhatia, A., Tesoriero, A. & Timmer, L. (2002). Evaluation of fungicides for control of Alternaria brown spot on Murcotts, 2001. Fungicide and Nematicide Tests (online) Report 57: M06. Crossref |
||||
| Bushong, P., & Timmer, L. (2000). Evaluation of postinfection control of citrus scab and melanose with benomyl, fenbuconazole, and azoxystrobin. Plant Disease, 84(11), 1246-1249. Crossref |
||||
| Cevheri, C. (2017). Preliminary Study on the Antifungal and Antimicrobial Activities of Some Medicinal Plants of Turkey. Journal of Biotechnology Research, 3(11), 110-113. | ||||
| Das, K., Tiwari, R. & Shrivastava, D. (2010). Techniques for evaluation of medicinal plant products as antimicrobial agents: current methods and future trends. Journal Medicinal Plants Research, 4(2), 104 -111. | ||||
| Dewdney, M., & Timmer, L. (2012). Florida Citrus Pest Management Guide: Citrus Scab. University of Florida. Factsheet. Pp. 146. | ||||
| Doidge, E. M. (1929). Some diseases of citrus prevalent in South Africa. South African journal of science, 26(12), 320-325. | ||||
| Domsch, K. H., Gams, W., & Anderson, T. H. (1980). Compendium of soil fungi. Volume 1, Academic Press, (London) Ltd. | ||||
| FAO, Food and Agriculture Organization (2013). Statistical Yearbook: World Food and Agriculture. Rome, FAO: 307. | ||||
| Fatima, N., Batool, H., Sultana, V., Ara, J., & Ehteshamul-Haque, S. (2009). Prevalence of post-harvest rot of vegetables and fruits in Karachi, Pakistan. Pak. J. Bot., 41(6), 3185-3190. | ||||
| Haleem, U., Mushtaq, K., Abbas, A., Sheikh, A., & Farooq, U. (2005). Estimation of Export Supply Function for Citrus Fruit in Pakistan [with Comments]. The Pakistan Development Review, Pp.659-672. | ||||
| Humber, R. A. (1997). Fungi: identification. Manual of techniques in insect pathology, Elsevier, 153-185. Crossref |
||||
| Jamadar, M., & Lingaraju, S. (2011). In vitro evaluation of fungicides, botanicals and bioagents against Elsinoe ampelina-An incitant of anthracnose of grapevine. Karnataka Journal of Agricultural Sciences, 24(2), 146-148. | ||||
| Johnson, D. A., Martin, M. & Cummings, T. F. (2003). Effect of chemical defoliation, irrigation water, and distance from the pivot on late blight tuber rot in center-pivot irrigated potatoes in the Columbia Basin. Plant disease, 87(8), 977-982. Crossref |
||||
| Johnson, G. (2006). Pakistan citrus industry challenges: Opportunities for Australia-Pakistan collaboration in research, development and extension. Pakistan: Citrus Industry Survey and Workshops. | ||||
| Khanzada, M., & Shah, G. S. (2012). In-vitro evaluation of fungicides, plant extracts and bio-controlagents against rice blast pathogen magnaporthe oryzae couch. Pak. J. Bot., 44(5), 1775-1778. | ||||
| Khanzada, M. A., Lodhi, A. M., & Shahzad, S. (2005). Chemical control of Lasiodiplodia theobromae, the causal agent of mango decline in Sindh. Pakistan Journal of Botany, 37(4), 1023. | ||||
| Kiely, T. (1964). Brown spot of Emperor mandarin. Agricultural Gazette of New South Wales, 75, 854-856. | ||||
| McKinney, H. (1923). A new system of grading plant diseases. J. Agric. Res, 26(2), 195-218. | ||||
| Miles, A., Willingham, S., & Cooke, A. (2005). Field evaluation of a plant activator, captan, chlorothalonil, copper hydroxide, iprodione, mancozeb and strobilurins for the control of citrus brown spot of mandarin. Australasian Plant Pathology, 34(1), 63-71. Crossref |
||||
| Murtaza, A., Shafique, S., Anjum, T., & Shafique, S. (2012). In vitro control of Alternaria citri using antifungal potentials of Trichoderma species. African Journal of Biotechnology, 11(42), 9985-9992. Crossref |
||||
| O'Rourke, T. A., Scanlon, T. T., Ryan, M. H., Wade, L. J., McKay, A. C., Riley, I. T., Li, H., Sivasithamparam, K., & Barbetti, M. J. (2009). Severity of root rot in mature subterranean clover and associated fungal pathogens in the wheatbelt of Western Australia. Crop and Pasture Science, 60(1), 43-50. Crossref |
||||
| Otani, H., Kohmoto, K., & Kodama, M. (1995). Alternaria toxins and their effects on host plants. Canadian Journal of Botany, 73(S1), 453-458. Crossref |
||||
| Pegg, K. (1996). Studies of a strain of Alternaria citri Pierce, the causal organism of brown spot of Emperor mandarin. Queensland Journal of Agricultural and Animal Sciences, 23,15-28. | ||||
| Pierce, N. B. (1902). Black rot of oranges. Botanical Gazette, 33(3), 234-235. Crossref |
||||
| Ploetz, R. C., & Gregory, N. (1992). Mango malformation in Florida: distribution of Fusarium subglutinans in affected trees, and relationships among strains within and among different orchards. IV International Mango Symposium, 341, 388-394. | ||||
| Ruehle, G. (1937). A strain of Alternaria citri Ellis and Pierce causing a leaf spot of rough lemon in Florida. Phytopathology, 27, 863-865. | ||||
| Safdar, A., Javed, N., Khan, S. A., Khan, H. U., Rehman, A., & Haq, I. (2010). Survey and investigation of different citrus growing areas for citrus sudden death syndrome. Pak. J. Phytopathol, 22(2), 71-78. | ||||
| Ploetz, R. C., & Gregory, N. (1992). Mango malformation in Florida: distribution of Fusarium subglutinans in affected trees, and relationships among strains within and among different orchards. IV International Mango Symposium, 341. | ||||
| Samson, R. A., Hoekstra, E. S., Frisvad, J. C., & Filtenborg, O. (1995). A Book Review: Introduction to Food-borne Fungi. Dairy Food and Environmental Sanitation, 15(7), 441-442. | ||||
| Samson, R. A., Hoekstra, E. S., Frisvad, C., & Fittenborg, C. (1995). Introduction to Food-borne Fungi. Centraalbureau voor Schimmelcultures, Baarn, The Netherlands. Wageningen, Ponsen and Looyen, 33-112. | ||||
| Timmer, L., Darhower, H., Zitko, S., Peever, T., Ibanez, A., & Bushong, P. (2000). Environmental factors affecting the severity of Alternaria brown spot of citrus and their potential use in timing fungicide applications. Plant Disease, 84(6), 638-643. Crossref |
||||
| Timmer, L., Mondal, S., Peres, N., & Bhatia, A. (2004). Fungal diseases of fruit and foliage of citrus trees. Diseases of Fruits and Vegetables, 1, 191-227. Crossref |
||||
| Timmer, L., & Zitko, S. (1992). Evaluation of fungicides for control of citrus scab, 1991. Fungicide and Nematicide Tests, 47, 334. | ||||
| Timmer, L. & Zitko, S. (1997). Evaluation of fungicides for control of Alternaria brown spot and citrus scab. Proceedings - Florida State Horticultural Society, 110, 71-76. | ||||
| Timmer, L. W., Peever, T. L., Solel, Z., & Akimitsu, K. (2003). Alternaria diseases of citrus-novel pathosystems. Phytopathologia Mediterranea, 42(2), 99-112. | ||||
| Whiteside, J. (1983). Fungicidal effects of some acaricides on Mycosphaerella citri. Plant disease, 67(8), 864-866. Crossref |
||||
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