Integrity Research Journals

ISSN: 2782-750X
Model: Open Access/Peer Reviewed
DOI: 10.31248/GJFS
Start Year: 2018

Comparative study of physico-chemical parameters and microbial load of aqua media of different receptacles used in culturing Clarias gariepinus   |   Article Number: 8F1156991   |   Vol.4 (4) - December 2022

Received Date: 27 October 2022   |   Accepted Date: 12 December 2022  |   Published Date: 30 December 2022

Authors:  Okeke, P. A.*, , Amuneke, K. E. and Evulobi, O. O. C.

Keywords: African catfish, microbial load, water quality., culture media, receptacles

The physico-chemical parameters cum microbial loads of water from earthen, concrete and collapsible plastic tank, tagged A, B and C respectively, were used to culture Clarias gariepinus at the Integrated Organic Fish Farm of the Department of Fisheries and Aquaculture, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria, which lasted for sixteen (16) weeks. Water samples were collected aseptically every week throughout the duration of the research. The mean values of physico-chemical parameters using analysis of variance (ANOVA), showed that there was no significant difference (p>0.05) in treatments A, B and C. The results showed that earthen pond recorded the highest in Biological Dissolved Oxygen (BOD) 3.67±0.417 mg/L, Sulphate 4.55±0.016 mg/L, Nitrate 3.12±0.023mg/L and Magnesium 1.28±0.042 mg/L, followed by B and C, while concrete pond recorded highest mean values in temperature 28.17±0.234°C, pH 7.10±0.252, alkalinity 31.17±0.189 mg/L, calcium 0.53±0.O27 mg/L, and potassium 11.68±0.016 mg/L followed by A and C. and collapsible plastic tank recorded highest mean values in dissolved oxygen DO 9.83±0.240 mg/L, and transparency 60.67±0.667 cm/L. The physico-chemical parameters were all within the accepted standard range by the World Health Organization (WHO) and Federal Emergency Protection Agency for culturing fish. The Least Significant Difference (LSD) showed that there were significant differences (p<0.05) between the bacteria load of water samples from A and C receptacles. It also, showed that there was no significant difference (p>0.05) between bacteria load of water samples from A and B receptacles. Water sample from A, had the highest CFU count of Escherichia coli, Staphylococcus sp., Streptococcus faecalis, Proteus sp, Clostridium perfringens and Pseudomonas aeruginosa of 0.62×107, followed by B water samples with Streptococcus faecalis, Staphylococcus sp. and Escherichia coli of 1.16 × 107 and C water samples E. coli and  Staphylococcus sp. with count of 1.78 × 107. Proper management of culturing media in different receptacles should be monitored to reduce microbial load in Clarias gariepinus, to avoid fish mortality and ill-health to human being when consumed.

APHA (2005). Standard method for the examination of water and waste water, 21st Edition, American Public Health Association. Pp. 62-68.
Bhatnagar, A., & Devi, P. (2013). Water quality guidance for the management of the pond fish culture. International Journal of Environmental Science, 3(6), 1980-1993.
Chukwura, E. I. (2001). Aquatic microbiology: Evaluation of surface water pollution. Published by Otoba Press LTD, Nkpor-Onitsha, Anambra State, Nigeria. Pp. 44-62.
Eze, J., & Ogbaran, K. (2010) Freshwater biodiversity conservation: Recent progress and future challenges in fish farming. Journal of Nature and Marine Conservation Benthol Social, 29(1), 344-358.
Ezenwa, B. I. O. (2006). Aquaculture research and fish farm development potentials in the Niger Delta. Paper presented at a workshop on Niger Delta fisheries potentials, 10th to May 2006. Port- Harcourt, Nigeria. Pp. 1-12.
Fafioye, O., Olurin, S. A., & Sowunmi, A. A. (2005).Studies on the physicochemical parameters (7 days variation) of a major water body of Ago-lwoye, Nigeria. Journal of Aquatic Science, 4(9), 1022-1024.
Fafioye, O. O. (2011). Preliminary studies on water characteristics and bacterial population in high yield Kajola fish ponds. Journal of Agricultural Extension and Rural Development, 3(3), 68-71.
FAO (2002). Food and agricultural publication, year report 2002. FAO Rome. Pp. 81-94.
FEPA (1991). Guidelines and standard for environmental pollution control. Federal Environmental Protection Agency, 27, 20-32.
King, R. P. (1998). Physico-chemical indices of the fisheries potential of a Nigerian rainforest pond. Journal of Aquatic Sciences, 13, 49-54.
Monaghen, J., & Hutchinson, M. (2010). Managing food safety risk in the agric-food industries. MA. USA Academic Press.
Njoku, P. (2015). Health implications of toxic water on fishes in Nigeria. European Scientific Journal, 8(8), 111-117.
Olayemi, A. B., & Opaleye, F. I. (1990). Antibiotic resistance among coliform bacteria isolated from hospital and urban wastewaters. World Journal of Microbiology and Biotechnology, 6(3), 285-288.
Okeke, P. A., Nwigwe, H. C., Nwosu, M. C. and Arazu, V. N. (2016). A comparative study on effects of artificial starter feeds on growth and survival of Clarias gariepinus fingerlings. Journal of Aquatic Sciences, 31(2B), 443-454.
Omojowo, F., & Omojasola, F. (2013). Antibiotic resistance pattern of bacterial pathogens isolated from poultry manure used to fertilize fish ponds in new Bussa, Nigeria. Albanian Journal of Agricultural Sciences, 12(1), 81-85.
Onome, A. D., & Ebinimi, A. (2010). Comparative assessment of water quality parameters of freshwater tidal earthen ponds and stagnant concrete tanks for fish production in Port Harcourt, Nigeria. International Journal of Science and Nature, 1(1), 34-37.
Osawe, M. (2004). Catfish fingerlings production management techniques. Success Attitude Development Centre, (SADC) Lagos, Nigeria. Workshop paper 32.
Phillips, I., Casewell, M., Cox, T., De Groot, B., Friis, C., Jones, R., Nightingale, C., Preston, R., & Waddell, J. (2004). Does the use of antibiotics in food animals pose a risk to human health? A critical review of published data. Journal of Antimicrobial Chemotherapy, 53(1), 28-52.
Poonkundran, T. (2019). Groundwater Quality analysis in and Around Chidambaram Taluk, Tamil Nadu. International Journal of Scientific and Research Publications, 9(7), 171-173.
Saloom, M. E., & Duncan, R. S. (2005). Low dissolved oxygen levels reduce antipredator behaviours of the fresh water Clam Corbicula fluminea. Freshwater Biology, 50(7), 1233-1238.
Slabyj, B. M., Martin, R. E., & Ramsdell, G. E. (1981). Reproducibility of Microbiological counts on frozen Cod: A collaborative study. Journal of Food Science, 46(3), 716-719.
Sterlings, M. J. (1985). Winkler titration method for dissolved oxygen determination. MDPI Journal.
Standard Organization of Nigeria (SON) (1970). Nigerian standard for drinking water quality. Standard Organization of Nigeria. Pp. 15-16.
Stone, N. M., & Thormforde, H. K. (2003). Understanding your fish pond water analysis report. University of Arkansas Co-operative Extension Printing services. Pp. 1-4.
Ugwu, L. L. C., & Mgbenka, B. O. (2006). Fisheries and wildlife management: A systematic approach. Published by Jones Communication Publishers, Edingburh Road, Enugu, Enugu State, Nigeria. Pp. 1-7.
World Health Organization (WHO) (2009). Guideline for drinking water quality. World Health Organization, Geneva. WHO/HSE/WSH/09.05. 39p.
Zmysłowska, I., Kolman, R., & Krause, J. (2003). Bacteriological evaluation of water, feed and sturgeon (Acipenser baeri Brandt) fry quality during intensive rearing in cooling water. Fisheries & Aquatic Life, 11(1), 91-98.