ISSN: 2992-4928
Model: Open Access/Peer Reviewed
DOI: 10.31248/AJPB
Start Year: 2019
Email: ajpb@integrityresjournals.org
https://doi.org/10.31248/AJPB2024.032 | Article Number: 48D0EB161 | Vol.4 (1) - June 2024
Received Date: 30 April 2024 | Accepted Date: 05 June 2024 | Published Date: 30 June 2024
Authors: Chidimma Iheanacho* , Paschal C. Akubuiro , Irene O. Oseghale , Vincent O. Imieje , Osayemwenre Erharuyi , Kennedy Ogbeide , Abiodun Falodun and Arthur Jideonwo
Keywords: Anacardium occidentale, phytosterols, stigmasterol, β-sitosterol.
The phytochemical screening of the stem bark extract of Anacardium occidentale revealed the presence of alkaloids, carbohydrates, reducing sugars, deoxysugars, saponins, phenolics, tannins, terpenoids, flavonoids, steroids, and proteins. This study aims to identify and characterize the bioactive principle from the stem bark of A. occidentale which is widely used in folk medicine. Column chromatography of the crude extracts led to several fractions. Thin Layer Chromatography (TLC) spotting and the spraying reagent (Concentrated H2SO4 and vanillin in methanol) were used to identify the fraction containing phytosterols. The isolation and purification yielded white crystalline powder which was subjected to physical, chemical, and spectral identification by IR, 1H‐NMR, and 13C‐NMR. The compound obtained was identified as a mixture of stigmasterol and β‐sitosterol.
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