JOURNAL OF PUBLIC HEALTH AND DISEASES
Integrity Research Journals

Model: Open Access/Peer Reviewed
DOI: 10.31248/JPHD
Start Year: 2016
Email: jphd@integrityresjournals.org


Investigation of the antibiotics susceptibility patterns and pathogenic potential of bacteria isolated from poultry wastes

https://doi.org/10.31248/JPHD2019.021   |   Article Number: 5DDF4BE43   |   Vol.1 (3) - December 2018

Received Date: 01 December 2018   |   Accepted Date: 27 December 2018  |   Published Date: 31 December 2018

Authors:  B. O. Uba* , E. L. Okoye , I. S. Etoniru , D. K. Anene and S. Ogbuagu

Keywords: Antibiotic resistance, gastrointestinal tract, pathogenicity, poultry wastes, public health.

The antibiotic resistant patterns and pathogenic potential of bacteria isolated from poultry wastes were investigated in this study. A total of 20 samples of poultry wastes were collected from different sites of poultry houses in Uli, Ihiala Local Government Areas, Anambra State, Nigeria using sterile polythene bags. The pathogens were isolated and identified using streaking plating technique, biochemical characterization and API Identification System. The disc diffusion method and plasmid curing were used to determine the antibiotic resistant patterns and resistant gene location. The pathogenicity study involves orally or intraperitonially inoculation of 0.5 mL of the different bacterial pathogens into three months’ immuno - competent albino mice for two weeks; and rabbit ileal loop assay for enterotoxin production in five healthy adult rabbit for 18 to 24 hrs. The isolated pathogens were Staphylococcus aureus, E. coli, Pseudomonas aeruginosa, Clostridium, Salmonella, Shigella, V. cholerae and V. parahaemolyticus. The antibiotics sensitivity test revealed that the bacterial pathogens were most susceptible to ciprofloxacin, rifampicin, levofloxacin and most resistant to ceporex, amoxil, ampiclox and norfloxacin with significance in the Gram negative antibiotics sensitivity testing (P < 0.05), but no significance in the Gram antibiotics sensitivity positive testing (P > 0.05). 12.5% of the strains lost their antibiotic resistance plasmids marker after sodium dodecyl sulfate (SDS) mediated curing. The pathogenicity testing revealed that the intestine had the highest significant microbial count (9 log CFU/g x 105) and the liver recorded the least significant count (7 log CFU/g x105) compare to the control (inoculated with water) that had no count. The enterotoxin testing revealed that Staphylococcus aureus recorded the highest enterotoxin production (0.45 mL/cm) while Pseudomonas aeruginosa recorded the least (0.16 mL/cm). Thus, the test isolates have shown reasonable pathogenic potentials and hence proper enlightenment and sensitization of the public health problems associated with these wastes should be encouraged.

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