JOURNAL OF DRUGS AND PHARMACEUTICAL SCIENCE
Integrity Research Journals

ISSN: 2705-2222
Model: Open Access/Peer Reviewed
DOI: 10.31248/JDPS
Start Year: 2016
Email: jdps@integrityresjournals.org


Anti-inflammatory and anti-nociceptive potentials of Salacia lehmbachii leaves

https://doi.org/10.31248/JDPS2017.009   |   Article Number: 10EAEEA81   |   Vol.1 (1) - October 2017

Received Date: 05 September 2017   |   Accepted Date: 25 September 2017  |   Published Date: 30 October 2017

Authors:  Augustine D. Essien* , Grace A. Essiet , Edidara E. Thomas , Francis V. Udoh and Godwin C. Akuodor

Keywords: Egg-albumin, herbal remedies, inflammation, carrageenan, analgesics, water extract, xylene.

For decades, the leaves of Salacia lehmbachii Loes (SL), which belong to the family of Celastraceae are acceptably used in south-eastern parts of Nigeria in traditional medicine for the treatment of fever and painful conditions. This study assessed the anti-inflammatory and anti-nociceptive effects of its water extracts on inflammation and nociception in rats. Doses of 100, 200 and 400 mg/kg of water extract of SL were used in elucidating anti-inflammatory activities in Wistar rats and mice by using carrageenan, egg albumin-induced paw and xylene-induced ear oedema; while acetic acid-induced writhing/abdominal muscle constriction, formalin-induced nociception and tail immersion models were used for analgesic study. In each study, 30 animals were randomly selected and grouped into five with six animals per group. Group 1(control) received 20 mL/kg of distilled water; groups 2 to 4 received 100, 200 and 400 mg/kg of the extract respectively; while group 5 received 150 mg/kg of aspirin and 10 mg/kg of morphine (tail immersion study). The ED50 of the water extract was 200 mg/kg. The water extract of the SL leaves showed significant (p<0.05) dose-dependent effects in all the parameters. Doses of 200 and 400 mg/kg showed comparable effects to those effects exhibited by the respective positive controls: - aspirin (anti-inflammatory study); aspirin and morphine (analgesic study). The water extract of the SL leaves possesses potent anti-inflammatory and analgesic properties. The results justify the claim by the herbalists and its use in folkloric medicines for pains and conditions associated with pains.

Agbaje, E. O., Adeneye, A. A., & Adeleke, T. I. (2008). Anti-nociceptive and anti-inflammatory effects of a Nigerian polyherbal tonic tea (PHT) extract in rodents. Afr. J. Trad. Comp. Alt. Med., 5(3), 247-256.
 
Ahmad, M., Mehjabeen, Zia-Ul-Haq, M., & Jahan, N. (2011). Determination of LD50 and ED50 by dose relationship and assessment of toxicological and non-toxicological behavior of Ipomoea hederacea. J. Pharmacol., 4(4), 1176-1178.
 
Akah, P. A., & Nwabie, A. (1994). Evaluation of Nigeria traditional medicine 1: plants used for rheumatic (inflammatory) disorders. J. Ethnopharmacol., 42, 179-182.
Crossref
 
Akuodor, G. C., Anyalewechi, N. A., Udoh, F. V., Ikoro, N. C., Akpan, J. L., Gwotmut, M. D., Iwuanyanwu, T. C., & Osunkwo, U. A. (2011). Pharmacological evaluation of Verbena hastate leaf extract in the relief of pain and fever. Adv. Pharmacol. Toxicol., 12(3), 1-8.
 
Akuodor, G. C., Essien, A. D., Udia, P. M., David-Oku, E., Chilaka, K. C., Asika, E. C., & Nwadum, S. K.. (2015). Analgesic, anti-inflammatory and antipyretic potential of the stem bark of Stachytarpheta indica. Br. J. Pharmacol. Toxicol., 6(1), 16-21.
Crossref
 
Al-Ghamdi M. S. (2001). Anti-inflammatory, analgesic and antipyretic activity of Nigella sativa. J. Ethnopharmacol., 76, 45- 48.
Crossref
 
Bachhav, R. S., Gulecha, V. S., & Upasani, C. D. (2009). Analgesic and anti-inflammatory activity of Argyrela speciosa root. Indian J. Pharmacol., 41(4), 158-161.
Crossref
 
Barnes, P. J., & Karin, M. (1998). Nuclear factors-kB -a pivotal transcription factor in chronic inflammatory diseases. N. Engl. J. Med., 336, 1066-1071.
Crossref
 
Breivik, H., Borchgrevink, P. C., Allen, S. M., Rosseland, L. A., Romunstad, L., & Hals, E. K. (2008). Assessment of pains. Br. J. Anaesthesia., 101(1), 17-24.
Crossref
 
Dhara, A. K., Sube, V., Sen, T., Pal, A. N., & Chandhiri, A. K. (2000). Preliminary studies on anti-inflammatory and analgesic activity of the methanolic fractions of the root extract of Tragia involucrate. J. Ethnopharmacol., 72, 265-268.
Crossref
 
Deraedt, R., Jongney, S., Delevalcee, F., & Falhout, M. (1980). Release of prostaglandin E and F in an analgesic reaction and its inhibition. Eur. J. Pharmacol., 51, 17-24.
Crossref
 
Duke, J. A. (1992). Hand book of Biologically Active Phytochemical and the Activities. CRC Press. Boca, Raton, FL. USA.
 
Edgar, J. D., Elias, B., & Adnah, B. (2002). Biotechnology and the developing world. Electronic J. Biotechnol., 5(1), 137-142.
 
Elujoba, A. A., Odeleye, O. M., & Ogunyemi, C. M. (2005). Traditional medicinal development for medical and dental primary health care delivery system in Africa. Afr. J. Trad. Complement. Alt. Med., 2, 46-61.
 
Essien, A. D., Akuodor, G. C., Essien, E. A., Asika, A. C., Chilaka, K. C., & Nwadum, S. K. (2015a). Evaluation of antipyretic potential of the ethanolic leaf extract of Salacia lehmbachiiLoes. Asian J. Med. Sci., 7(2), 22- 25.
Crossref
 
Essien, A. D., Lapah, P. T., & Emmanuel, I. A. (2015b). In vitro cholinergic and acute toxicity of Salacia lehmbachii. International Journal of Pharmacy and Pharmaceutical Research, 5(1), 200-207.
 
Essien, A. D., Essiet, G. A., Akuodor, G. C., Aja, D. O. J., Thomas, E. E., Nwadike, K. I., Nwachukwu, D. C., & Chilaka, K. C. (2016a). Studies on gastrointestinal properties of ethanolic leaf extract of Salacia lehmbachii in Wister rats. Afr. J. Pharmacy Pharmacol., 10(20), 451-457.
Crossref
 
Essien, A. D., Essiet, G. A., Akuodor, G. C., Akpan, J. L., Chilaka, K. C., Bassey, A. L., Ezeokpo, B. C., & Nwobodo, N. N. (2016b). Pharmacological evaluation of the aqueous stem bark extract of Bombax buonopozense in the relief of pain and fever. Afr. J. Pharmacy Pharmacol., 10(5), 59-65.
Crossref
 
Essiet, G. A., Takem, L. P., Essien, A. D., Akuodor, G. C., & Udoh, F. V. (2016a). Investigation of hepatic toxicity of Salacia lehmbachii. International Journal of Pharmacy and Pharmaceutical Research, 6(2), 95-103.
 
Essiet, G. A., Essien, A. D., Takem, L. P., Akuodor, G. C., & Udoh, F. V. (2016b). Hematological changes following oral administration of aqueous root bark extract of Salacia lehmbachii in albino rats. Int. Blood Res. Review, 6(1), 1-8.
Crossref
 
Fields, H. L., & Basbaum, A. I. (1994). Central Nervous mechanisms of pain modulation. In: Wall, P. D., Melzack R., (ed.) Text book of Pain. Elsevier/Churchill Livingstone, Edinburgh.
 
Ganesh, M., Vasudevan, M., Kamalakaman, K., Kamar, A. S., Vinoba, M., Gaguly, S., & Sivakumar, T. (2008). Anti-inflammatory and analgesic effects of Pongamia glabra leaf gall extract. Pharmacol. Online, 1, 497-512.
 
Ghosh, M. N. (2005). Fundamental of Experimental pharmacology. India: Scientific Book Agency. Calcutta. Pp. 69-71.
 
Hakiman, M., & Maziah, M. (2009). Non enzymatic and antioxidant activities in aqueous extract of different Ficudeltoidea accessions. J. Med. plant Res., 3(3), 120-131.
 
Hollander-Hadacek, F. (2002). Secondary metabolites as plant traits: Current assessment and future perspectives. Crit. Rev. plant Sci., 21, 273- 322.
Crossref
 
Hosseinzedeh, H., & Younesi, H. M. (2002). Anti-nociceptive and anti-inflammatory effects of Crocus sativus L stiga and petal extracts in mice. BMC Pharmacol., 2(7), 1471-2210.
 
Huffman, M. A. (2003). Animal self-medication and ethno-medicine exploration and exploitation of the medicinal properties of plants (in Hindu). Proc. Nutr. Soc., 62(2), 371- 381.
Crossref
 
Jumping, K., Yun, N., Wang, N., Liang, I., & Zhi-Hong, H. (2005). Analgesic and anti-inflammatory activities of total extract and individual fractions of Chinese medicinal plant Polyrrchasis lamellidens. Biol. Pharmaceut. Bull., 28, 176- 180.
Crossref
 
Lai, P. K., & Roy, J. (2004). Antimicrobial and chemo-preventive properties of herbs and spices. Curr. Med. Chem., 11(11), 451-460.
Crossref
 
Laurence, D. R., & Bennett, P. N. (1994). Clinical Pharmacology. 7th ed. ELBS Churchill Livingstone. UK.
 
Lin, L. L., Lin, A. Y., & Knopt, J. L. (1992). Cytosolic phospholipase A2 is coupled to hormonally regulated release of arachidonic acid. Proc. Natl. Acad. Sci., 89, 6147-6517.
Crossref
 
Luster, A. D. (1998). Mechanisms of disease: Chemokines-Chemotactic cytokines that mediate inflammation. N. Engl. J. Med., 338, 436-445.
Crossref
 
Mukherjee, P. R. (2006). Quality Control of Herbal Drugs, an approach to Evaluation of Botanicals. 13th Ed., Business Horizones Publishers, New Delhi. Pp. 419-459.
 
Mutalik, S., Paridhavi, K., Mallikarjuna, R. C., & Udupa, N. (2003). Antipyretic and analgesic effect of leaves of Solamummelongena Linn in rodents. Indian J. Pharmacol., 35, 312-315.
 
OECD (2010). Guidelines for the testing of chemicals. Testing no. 423 acute oral toxicity. Acute Toxicity Class. Method. 1(4), 1- 14.
 
Okokon, J. E., & Nwafor, P. A. (2010). Anti-Inflammatory, Analgesic and Antipyretic activities of ethanolic root extract of Croton zambesicus. Pak. J. Pharm. Sci. 23(4), 385-392.
 
Ramabadran, K., Bansinath, M., Turndorf, H., & Puig, M. H. (1989). Tail immersion test for the evaluation of nociceptive reactions in mice. J. Pharmacol. Methods, 21(1), 21-31.
Crossref
 
Rang, H. P., Dale, M. M., & Ritter, J. M. (1999). Pharmacology. Churchill Livingstone; 4th edit. Pp. 198-602.
 
Ravelo, Y., Molina, V., Carbajal, D., Fernandez, L., Fernandez, J., Arruzazabala, M. L., & Mas, R. (2011). Evaluation of anti-inflammatory and anti-nociceptive effects of D-002 (beeswax alcohols). J. Nat. Med. 65(2), 330-335.
Crossref
 
Singh, A., Malhotra, S., & Subban, R. (2008). Anti-inflammatory and analgesic agents from Indian medicinal plants. Int. J. Integr. Biol., 3(1), 57- 60.
 
Singh, S. D., & Majumbar, K. (1995). Analysis of activity of Olimum sanctum and its possible mechanism of action. Int. J. Pharmacol., 3, 188-192.
 
Sofowora, A. (1993). Medicinal Plants and Traditional Medicine in Africa. Spectrum Books, Ibadan. p. 150.
 
Tapsel, L. C., Hemphil, A., & Cobiac, L. (2006). Health benefits of herbs and spices: The past, the present and the future. Med. J. Austr., 185(4), 24-54.
 
Tjolsen, A., Berge, O. G., Hunskaar, S., Rasland, J. H., & Hole, K. (1992). The formalin test: An evaluation of method. Pain. 51, 5-17.
Crossref
 
WHO (2006a). Guidelines for the treatment of malaria, Geneva. Willcox, M. L., & Bodeker, G. (2004). Clinical review: Traditional herbal medicines for malaria. BMJ, 329, 1156-1163.
 
Xu, Q., Wang, Y., Guo, S., Shen, Z., Wang, Y., & Yang, L. (2014). Anti-inflammatory and analgesic activity of water extract of Flospopuli. J. Ethnopharmacol., 152(3), 540-545.
Crossref